| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,157 | C→T | 9.7% | D48D (GAC→GAT) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,157 | 0 | C | T | 9.7% | 345.4 / 18.7 | 113 | D48D (GAC→GAT) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (48/54); new base T (4/7); total (52/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 114 total aligned reads displayed.
ATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTC > minE/218090‑218224 | atatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAg > 1:3181590/1‑70 (MQ=255)atatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAg < 1:2291658/70‑1 (MQ=255)atatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:3355322/71‑1 (MQ=255)atatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:403665/71‑1 (MQ=255) tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAATATGCGAAAAGCAGCCATGGACAGaa < 1:647353/71‑1 (MQ=255) tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:145670/70‑1 (MQ=255) atTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGAc > 1:3642772/1‑66 (MQ=255) atTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:2332996/1‑71 (MQ=255) ttttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:3254819/68‑1 (MQ=255) ttttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAc < 1:3823550/71‑1 (MQ=255) ttttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAc > 1:789687/1‑71 (MQ=255) tttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:1430416/67‑1 (MQ=255) ttaaCGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACt < 1:2144779/67‑1 (MQ=255) tGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTa < 1:291078/70‑1 (MQ=255) aaCGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTatc > 1:2302951/2‑69 (MQ=255) aaCGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTatc > 1:1913283/2‑69 (MQ=255) aCGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAc < 1:3040248/66‑1 (MQ=255) aCGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAc < 1:3789528/66‑1 (MQ=255) aaTGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGc < 1:1917859/71‑1 (MQ=255) aTGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCg < 1:1117020/71‑1 (MQ=255) aTGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCg > 1:3207457/1‑71 (MQ=255) tGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGt > 1:2223288/1‑71 (MQ=255) tGAGAAGGTATTAAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACt > 1:2022421/1‑62 (MQ=255) aGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCt > 1:465618/1‑61 (MQ=255) ggTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:3102749/1‑54 (MQ=255) gTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCt > 1:2293620/1‑71 (MQ=255) gTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGAACAGAAACTACCTGGCGTCAGTCCt > 1:3230561/1‑71 (MQ=255) tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCtt > 1:2291435/1‑71 (MQ=255) aTTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTg > 1:807176/1‑71 (MQ=255) aTTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTg > 1:1203054/1‑71 (MQ=255) aTTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTg > 1:4119509/1‑71 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:1340411/48‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:4091948/48‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:3678727/48‑1 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:2014657/1‑48 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:3890734/1‑48 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCt < 1:3415655/54‑1 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCg > 1:187658/1‑70 (MQ=255) aaGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:2562296/1‑47 (MQ=255) ggCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCggg > 1:1901107/1‑69 (MQ=255) cAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGc < 1:833421/71‑1 (MQ=255) aGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGt < 1:2840579/53‑1 (MQ=255) aGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCg < 1:4057331/71‑1 (MQ=255) aGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCg < 1:3230083/71‑1 (MQ=255) gggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgggg < 1:4075973/66‑1 (MQ=255) ggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGc < 1:3726520/68‑1 (MQ=255) gTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGa < 1:600666/65‑1 (MQ=255) gTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGa < 1:1968890/65‑1 (MQ=255) gTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:2666520/1‑39 (MQ=255) gTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTAc > 1:4132895/1‑43 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:1003594/36‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:122705/36‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCt < 1:196569/71‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgg > 1:1851928/1‑61 (MQ=255) tAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCtt > 1:1841244/1‑71 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCttt > 1:2480533/1‑71 (MQ=255) aGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACt < 1:2932131/37‑1 (MQ=255) aGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCggg < 1:1726861/59‑1 (MQ=255) aGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:1368511/1‑58 (MQ=255) aGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:377940/1‑58 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg < 1:3654419/71‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCt > 1:2426795/1‑38 (MQ=38) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGTAGCGGGCTTTCgta < 1:3869391/71‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgt < 1:1015421/70‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgt < 1:2551760/70‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg > 1:1410887/1‑69 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCg < 1:3732799/53‑1 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg < 1:3817410/45‑1 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg < 1:1195059/45‑1 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg < 1:1199974/45‑1 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGa > 1:753863/1‑56 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtagt < 1:1559773/71‑1 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtagt < 1:308399/71‑1 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtagt < 1:2904104/71‑1 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtag > 1:3508145/1‑70 (MQ=255) aaGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg > 1:665057/1‑66 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGc < 1:849033/56‑1 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTc > 1:675423/1‑40 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtta < 1:3404136/48‑2 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTG‑GGGGAGCGGGCTTTCGTAGTaa > 1:457266/1‑70 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgg > 1:3920696/1‑51 (MQ=255) gATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg < 1:3550700/64‑1 (MQ=255) gCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgta > 1:3530399/1‑63 (MQ=255) gAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtta < 1:2863869/42‑2 (MQ=255) gAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCgtaatc < 1:3379625/64‑4 (MQ=255) aaaaGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGc < 1:2598712/42‑1 (MQ=255) aaaaGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGc < 1:2527603/42‑1 (MQ=255) aaaaGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAg < 1:772944/48‑1 (MQ=255) aaGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCg > 1:2681255/1‑41 (MQ=255) aaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGa > 1:1611346/1‑70 (MQ=255) cagcCATGGACAGAAACTACCTGGCGTCAGTACTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGActct > 1:1312792/1‑71 (MQ=255) gcCATGGACAGAAACTACCTGGCGTCAGTGCTTGCGGGGAGCGGGCTTTCgtagt < 1:3970781/55‑1 (MQ=255) gcCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTcc > 1:1153224/1‑71 (MQ=255) ccATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATAcccc > 1:1669971/1‑62 (MQ=255) ccATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTccc < 1:4069344/71‑1 (MQ=255) aTGGATATAAACTATCTCGCGTCAGTCCTTGCGGGGa < 1:2497878/37‑1 (MQ=25) ggACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAg < 1:3851405/36‑1 (MQ=255) ggACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg < 1:811416/46‑1 (MQ=255) gACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTc < 1:1583840/70‑1 (MQ=255) gACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAG‑GGGCTTTCGTAGTAATACCCCGa > 1:567215/1‑58 (MQ=255) | ATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTC > minE/218090‑218224 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |