| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,333 | A→C | 18.2% | A323A (GCT→GCG) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,333 | 0 | A | C | 18.2% | 157.4 / 38.9 | 77 | A323A (GCT→GCG) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base A (30/33); new base C (5/9); total (35/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACT > minE/1439267‑1439403 | gTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAc > 1:643447/1‑71 (MQ=255) tGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc > 1:2809950/1‑70 (MQ=255) catcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaat < 1:2924583/68‑2 (MQ=17) catcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaat < 1:1463257/68‑2 (MQ=17) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:973159/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatat > 1:1123461/1‑71 (MQ=18) gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatat > 1:1640258/1‑71 (MQ=18) gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaata < 1:2054752/70‑3 (MQ=18) gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaata < 1:2530049/70‑3 (MQ=18) agGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACata > 1:3505148/1‑71 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaac < 1:3488174/60‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt < 1:3667023/71‑1 (MQ=255) gTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGcc < 1:2490528/57‑1 (MQ=14) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggta > 1:1740684/1‑50 (MQ=255) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:3466632/1‑70 (MQ=255) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:3326456/1‑70 (MQ=255) gATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAttta < 1:2795632/70‑2 (MQ=17) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:3713999/71‑1 (MQ=255) ttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGa > 1:989524/1‑70 (MQ=255) ttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGa > 1:3307598/1‑70 (MQ=255) ttGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:3859438/1‑65 (MQ=17) gTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGat < 1:2152914/69‑1 (MQ=255) tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGatat < 1:757280/70‑1 (MQ=255) gTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCt < 1:61310/70‑1 (MQ=255) gTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCt < 1:1263415/70‑1 (MQ=255) gTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCt < 1:2311225/70‑1 (MQ=255) tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt < 1:1438435/58‑1 (MQ=255) tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtc < 1:3228435/71‑1 (MQ=255) tCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatc < 1:3065161/50‑1 (MQ=11) cAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCtt > 1:2219639/1‑69 (MQ=255) cAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg > 1:1811161/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTTTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtc > 1:3473268/1‑69 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGatat > 1:3354198/1‑65 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:2967297/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:627788/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:2702276/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaa > 1:1669437/1‑44 (MQ=11) aaCGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaa > 1:3953001/1‑44 (MQ=11) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGat < 1:553647/61‑1 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt < 1:2832163/71‑1 (MQ=255) tAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc < 1:3108987/54‑1 (MQ=255) ggTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTcgtcgtcgt < 1:2130629/71‑1 (MQ=255) gTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:1505036/53‑1 (MQ=255) tGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt < 1:1831647/48‑1 (MQ=255) ggACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa > 1:1448028/1‑41 (MQ=255) gACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTcgtcgtcgt > 1:1593688/1‑67 (MQ=255) gACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGc > 1:865785/1‑70 (MQ=255) gACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGc < 1:655539/70‑1 (MQ=255) aCATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGc < 1:417207/69‑1 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc < 1:118860/71‑1 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc > 1:1207108/1‑71 (MQ=255) cATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc > 1:1799226/1‑71 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacgt < 1:3877550/40‑3 (MQ=18) tGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca < 1:2117872/38‑1 (MQ=255) tGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:2132664/46‑1 (MQ=255) tGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:702818/46‑1 (MQ=255) tGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgtc < 1:2729639/61‑1 (MQ=255) gTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAc > 1:3050398/1‑71 (MQ=255) tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc > 1:3367467/1‑71 (MQ=255) tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc > 1:2397626/1‑71 (MQ=255) tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc > 1:4108720/1‑71 (MQ=255) ttttGTTGAAGTAGTAGGTCGCACCAATATCAACGTAttta < 1:4015651/41‑2 (MQ=21) ttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGatat > 1:768473/1‑48 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAccc < 1:1544930/70‑1 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAccc < 1:544190/70‑1 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc > 1:2273403/1‑69 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc > 1:2045107/1‑69 (MQ=255) ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGa > 1:2302862/1‑43 (MQ=255) ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGa > 1:522397/1‑43 (MQ=255) aaGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGttttt < 1:2768811/71‑1 (MQ=255) aaGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGtttt < 1:912838/70‑1 (MQ=255) agtagtAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGttttt < 1:132807/70‑1 (MQ=255) agtagtAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTa < 1:4101981/71‑1 (MQ=255) gtagtaGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAccc < 1:3792926/61‑1 (MQ=255) gtagtaGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAccc < 1:1277487/61‑1 (MQ=255) tagtagGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAg < 1:358433/62‑1 (MQ=255) tagtagGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTa < 1:3307129/69‑1 (MQ=255) agtagGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCt > 1:1883901/1‑71 (MQ=255) gtaggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGGCACGACCCAGGt > 1:1870377/1‑62 (MQ=255) gtaggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCtt < 1:1794312/71‑1 (MQ=255) taggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCttt < 1:932219/71‑1 (MQ=255) ggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCtt > 1:1468403/1‑68 (MQ=255) ggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTa < 1:1791809/70‑1 (MQ=255) ggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTa < 1:2053667/70‑1 (MQ=255) ggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTa < 1:2845870/70‑1 (MQ=255) ggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTa < 1:2863106/70‑1 (MQ=255) gtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTa < 1:3580281/69‑1 (MQ=255) tagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGa > 1:3166963/1‑70 (MQ=255) agCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACt > 1:1707152/1‑71 (MQ=255) | GTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACT > minE/1439267‑1439403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |