| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,444 | G→A | 8.7% | Y286Y (TAC→TAT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,444 | 0 | G | A | 8.7% | 181.1 / 5.0 | 57 | Y286Y (TAC→TAT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (27/25); new base A (2/3); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTG > minE/1439375‑1439513 | gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:1418986/70‑1 (MQ=255)gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:2790721/70‑1 (MQ=255) cccAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGc < 1:186307/71‑1 (MQ=255) cAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa < 1:2900696/71‑1 (MQ=255) aGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa > 1:2467765/1‑70 (MQ=255) ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa > 1:2653260/1‑69 (MQ=255) gTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAg > 1:1684937/1‑71 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt > 1:3009260/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGcg < 1:1836276/70‑2 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt > 1:357376/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt < 1:855093/70‑1 (MQ=255) aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:4000594/70‑1 (MQ=255) ccTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa > 1:3401434/1‑61 (MQ=255) ccTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa < 1:1426935/70‑1 (MQ=255) tAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg > 1:1024019/1‑68 (MQ=255) aGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc < 1:966320/70‑1 (MQ=255) aGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc < 1:3420331/70‑1 (MQ=255) aGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCt > 1:1810798/1‑71 (MQ=255) tGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGc > 1:1274405/1‑71 (MQ=255) cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTAGAAGTTc < 1:551192/64‑1 (MQ=255) ggTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGt < 1:261651/60‑1 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa < 1:276747/47‑1 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg > 1:1665663/1‑70 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:3461042/1‑71 (MQ=255) tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt < 1:2011551/71‑1 (MQ=255) aaGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGttttct > 1:2652852/1‑67 (MQ=9) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTgg > 1:833724/1‑35 (MQ=255) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAGGTTCTGTGCTTTGTTc > 1:1056659/1‑71 (MQ=255) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc > 1:3070839/1‑71 (MQ=255) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc > 1:1658874/1‑71 (MQ=255) ccAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt < 1:4109220/67‑1 (MQ=255) cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGt < 1:1909043/53‑1 (MQ=255) cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGt < 1:3210596/53‑1 (MQ=255) cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt > 1:2892149/1‑61 (MQ=255) aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa < 1:179870/71‑1 (MQ=255) gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:1160238/62‑1 (MQ=255) gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:1419433/62‑1 (MQ=255) ggacggacGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAc > 1:149465/1‑71 (MQ=255) acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGaa > 1:123236/1‑46 (MQ=255) acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGaa > 1:782062/1‑46 (MQ=255) acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGaa > 1:3875356/1‑46 (MQ=255) ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:1778484/42‑1 (MQ=255) gacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg < 1:171568/71‑1 (MQ=255) gacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:3998221/1‑71 (MQ=255) cgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGa < 1:1212527/71‑1 (MQ=255) gCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGaa < 1:2218482/40‑1 (MQ=255) aGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAAc > 1:289134/1‑70 (MQ=255) gACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg > 1:31521/1‑64 (MQ=255) caaaGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc > 1:3545070/3‑52 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt > 1:192512/1‑47 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt > 1:4106496/1‑47 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:3117034/1‑46 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgcg < 1:2940728/71‑1 (MQ=255) aGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAAcc < 1:3986003/64‑1 (MQ=255) aGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc < 1:3846794/49‑1 (MQ=16) aGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc < 1:859923/49‑1 (MQ=16) tCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:2165320/42‑1 (MQ=255) gAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTg > 1:2052527/1‑71 (MQ=255) gAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTg > 1:669401/1‑71 (MQ=255) cTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt < 1:3270655/39‑1 (MQ=12) ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAc < 1:379509/58‑1 (MQ=255) ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAc < 1:2111609/58‑1 (MQ=255) gTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGTGAACCTACGCGAGTTGCGtt < 1:2735097/69‑1 (MQ=255) gTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGtt < 1:2660799/69‑1 (MQ=255) gTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTg < 1:681393/70‑1 (MQ=255) gTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTg < 1:3648082/70‑1 (MQ=255) | GCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTG > minE/1439375‑1439513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |