| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,471 | T→G | 6.1% | A277A (GCA→GCC) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,471 | 0 | T | G | 6.1% | 225.2 / 9.0 | 82 | A277A (GCA→GCC) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base T (44/33); new base G (2/3); total (46/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGG > minE/1439403‑1439540 | tGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGc > 1:1274405/1‑71 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg > 1:1665663/1‑70 (MQ=255) gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:3461042/1‑71 (MQ=255) tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt < 1:2011551/71‑1 (MQ=255) aaGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGttttct > 1:2652852/1‑67 (MQ=9) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAGGTTCTGTGCTTTGTTc > 1:1056659/1‑71 (MQ=255) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc > 1:1658874/1‑71 (MQ=255) aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc > 1:3070839/1‑71 (MQ=255) ccAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt < 1:4109220/67‑1 (MQ=255) cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt > 1:2892149/1‑61 (MQ=255) aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa < 1:179870/71‑1 (MQ=255) gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:1419433/62‑1 (MQ=255) gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:1160238/62‑1 (MQ=255) ggacggacGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAc > 1:149465/1‑71 (MQ=255) gacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:3998221/1‑71 (MQ=255) gacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg < 1:171568/71‑1 (MQ=255) cgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGa < 1:1212527/71‑1 (MQ=255) aGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAAc > 1:289134/1‑70 (MQ=255) gACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg > 1:31521/1‑64 (MQ=255) caaaGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc > 1:3545070/3‑52 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt > 1:192512/1‑47 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt > 1:4106496/1‑47 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt > 1:3117034/1‑46 (MQ=255) aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgcg < 1:2940728/71‑1 (MQ=255) aGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAAcc < 1:3986003/64‑1 (MQ=255) aGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc < 1:3846794/49‑1 (MQ=16) aGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc < 1:859923/49‑1 (MQ=16) tCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg < 1:2165320/42‑1 (MQ=255) gAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTg > 1:669401/1‑71 (MQ=255) gAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTg > 1:2052527/1‑71 (MQ=255) cTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt < 1:3270655/39‑1 (MQ=12) ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAc < 1:379509/58‑1 (MQ=255) ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAc < 1:2111609/58‑1 (MQ=255) gTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGTGAACCTACGCGAGTTGCGtt < 1:2735097/69‑1 (MQ=255) gTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGtt < 1:2660799/69‑1 (MQ=255) gTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTg < 1:681393/70‑1 (MQ=255) gTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTg < 1:3648082/70‑1 (MQ=255) tACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTg > 1:288948/1‑69 (MQ=255) cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAgg > 1:216314/1‑71 (MQ=255) gAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTc > 1:1691355/1‑71 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCaa > 1:2115172/1‑37 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCt > 1:395924/1‑49 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGtt < 1:1796790/63‑1 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTc > 1:4046781/1‑70 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt > 1:1684308/1‑71 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt > 1:493184/1‑71 (MQ=255) aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt > 1:594248/1‑71 (MQ=255) cAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:597374/1‑41 (MQ=255) cAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg > 1:686870/1‑41 (MQ=255) cAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCt > 1:3734296/1‑69 (MQ=255) cAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTgg < 1:3340915/71‑1 (MQ=255) cAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTgg < 1:1491548/71‑1 (MQ=255) cAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTgg < 1:2002852/71‑1 (MQ=255) aaCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg > 1:2134187/1‑39 (MQ=255) aCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgt > 1:445605/1‑71 (MQ=255) aCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgt > 1:1253464/1‑71 (MQ=255) cAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg < 1:1594091/71‑1 (MQ=255) cAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg < 1:3738859/71‑1 (MQ=255) aGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg > 1:536009/1‑70 (MQ=255) gCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAAc > 1:1534145/1‑40 (MQ=255) gCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTg < 1:1737911/57‑1 (MQ=255) gCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTa > 1:1884437/1‑59 (MQ=255) gCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTa > 1:4096178/1‑59 (MQ=255) gCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTa < 1:2900689/71‑1 (MQ=255) ttCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAAc > 1:1849278/1‑38 (MQ=255) ttCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTa > 1:1084236/1‑57 (MQ=255) gAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTg < 1:217462/52‑1 (MQ=255) aGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGa > 1:1250944/1‑41 (MQ=255) aGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGag > 1:1880926/1‑69 (MQ=255) aGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGag > 1:3187340/1‑69 (MQ=255) aGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGag > 1:1664102/1‑69 (MQ=255) ttCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgcg < 1:1714729/38‑1 (MQ=255) ttCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCg < 1:3574368/45‑1 (MQ=255) ttCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGt < 1:3518658/46‑1 (MQ=255) ttCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGt < 1:2793439/46‑1 (MQ=255) ttCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGt < 1:1607860/46‑1 (MQ=255) tCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcagc > 1:3747784/1‑70 (MQ=255) cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAg > 1:1456094/1‑38 (MQ=255) cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCg > 1:2066152/1‑43 (MQ=255) cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg < 1:313591/58‑1 (MQ=255) cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg < 1:276540/58‑1 (MQ=255) cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGcc < 1:2799660/70‑1 (MQ=255) tgCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGt < 1:1048692/44‑1 (MQ=255) tgCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAgg > 1:1566981/1‑70 (MQ=255) tgCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAgg > 1:4073929/1‑70 (MQ=255) tgCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAgg < 1:1850245/70‑1 (MQ=255) | TGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGG > minE/1439403‑1439540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |