| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,166 | C→T | 18.7% | Y51Y (TAC→TAT) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,166 | 0 | C | T | 18.7% | 215.0 / 39.3 | 91 | Y51Y (TAC→TAT) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (39/35); new base T (5/12); total (44/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
AATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACA > minE/218101‑218234 | aaTGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGc < 1:1917859/71‑1 (MQ=255) aTGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCg > 1:127160/1‑71 (MQ=255) aTGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCg < 1:1117020/71‑1 (MQ=255) aTGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCg > 1:3207457/1‑71 (MQ=255) tGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGt > 1:2223288/1‑71 (MQ=255) aGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCt > 1:465618/1‑61 (MQ=255) gTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCt > 1:2293620/1‑71 (MQ=255) gTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGAACAGAAACTACCTGGCGTCAGTCCt > 1:3230561/1‑71 (MQ=255) tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCtt > 1:2291435/1‑71 (MQ=255) aTTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTg > 1:3813829/1‑71 (MQ=255) aTTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTg > 1:807176/1‑71 (MQ=255) aTTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTg > 1:1203054/1‑71 (MQ=255) aTTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTg > 1:4119509/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCt < 1:3415655/54‑1 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCg > 1:187658/1‑70 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCTTTGCgg > 1:675992/1‑71 (MQ=255) ggCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCggg > 1:1901107/1‑69 (MQ=255) cAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGc < 1:833421/71‑1 (MQ=255) aGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGt < 1:2840579/53‑1 (MQ=255) aGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCg < 1:4057331/71‑1 (MQ=255) aGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCg < 1:3230083/71‑1 (MQ=255) gggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgggg < 1:4075973/66‑1 (MQ=255) ggTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGc < 1:3726520/68‑1 (MQ=255) gTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGa < 1:600666/65‑1 (MQ=255) gTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGa < 1:1968890/65‑1 (MQ=255) gTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTAc > 1:4132895/1‑43 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCt < 1:196569/71‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgg > 1:1851928/1‑61 (MQ=255) tAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCtt > 1:1841244/1‑71 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCttt > 1:2480533/1‑71 (MQ=255) aGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCggg < 1:1726861/59‑1 (MQ=255) aGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:1368511/1‑58 (MQ=255) aGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:377940/1‑58 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg < 1:3654419/71‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCt > 1:2426795/1‑38 (MQ=38) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGTAGCGGGCTTTCgta < 1:3869391/71‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgt < 1:1015421/70‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgt < 1:2551760/70‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg > 1:1410887/1‑69 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCg < 1:3732799/53‑1 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg < 1:3817410/45‑1 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg < 1:1199974/45‑1 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg < 1:1195059/45‑1 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGa > 1:753863/1‑56 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtagt < 1:1559773/71‑1 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtagt < 1:2904104/71‑1 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtagt < 1:308399/71‑1 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtag > 1:3508145/1‑70 (MQ=255) aaGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtagt > 1:851487/1‑70 (MQ=255) aaGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg > 1:665057/1‑66 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGc < 1:849033/56‑1 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTc > 1:675423/1‑40 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtta < 1:3263540/48‑2 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtta < 1:3404136/48‑2 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTG‑GGGGAGCGGGCTTTCGTAGTaa > 1:457266/1‑70 (MQ=255) aGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgg > 1:3920696/1‑51 (MQ=255) gATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCggg > 1:434918/1‑51 (MQ=255) gATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg < 1:3550700/64‑1 (MQ=255) aTGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTc < 1:3601167/42‑1 (MQ=255) gCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgta > 1:3530399/1‑63 (MQ=255) gAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtta < 1:2863869/42‑2 (MQ=255) gAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCgtaatc < 1:3379625/64‑4 (MQ=255) aaaaGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGc < 1:2598712/42‑1 (MQ=255) aaaaGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGc < 1:2527603/42‑1 (MQ=255) aaaaGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAg < 1:772944/48‑1 (MQ=255) aaaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgt > 1:2063674/1‑58 (MQ=255) aaGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCg > 1:2681255/1‑41 (MQ=255) aaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGa > 1:1611346/1‑70 (MQ=255) cagcCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgt < 1:2399621/54‑1 (MQ=255) cagcCATGGACAGAAACTACCTGGCGTCAGTACTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGActct > 1:1312792/1‑71 (MQ=255) gcCATGGACAGAAACTACCTGGCGTCAGTGCTTGCGGGGAGCGGGCTTTCgtagt < 1:3970781/55‑1 (MQ=255) gcCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTcc > 1:1153224/1‑71 (MQ=255) ccATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATAcccc > 1:1669971/1‑62 (MQ=255) ccATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTccc < 1:4069344/71‑1 (MQ=255) ccATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTccc > 1:264221/1‑71 (MQ=255) aTGGATATAAACTATCTCGCGTCAGTCCTTGCGGGGa < 1:2497878/37‑1 (MQ=25) ggACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAg < 1:3851405/36‑1 (MQ=255) ggACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg < 1:811416/46‑1 (MQ=255) gACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTc < 1:1583840/70‑1 (MQ=255) gACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAG‑GGGCTTTCGTAGTAATACCCCGa > 1:567215/1‑58 (MQ=255) aGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtag > 1:1670170/1‑45 (MQ=255) aGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTccc > 1:4109688/1‑63 (MQ=255) aGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCtt > 1:2025505/1‑70 (MQ=255) aGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTa < 1:1098499/71‑1 (MQ=255) gAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATAc < 1:3293331/50‑1 (MQ=255) gAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTa < 1:1166993/70‑1 (MQ=255) aaCTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATAccc > 1:3564837/1‑50 (MQ=255) aCTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTaa > 1:3507135/1‑44 (MQ=255) aCTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTcccc < 1:24954/60‑1 (MQ=255) aCTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTc > 1:2463527/1‑63 (MQ=255) aCTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAaca < 1:1809961/71‑1 (MQ=255) cTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAacac < 1:42574/71‑1 (MQ=255) ccTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAAcaca < 1:473885/69‑1 (MQ=255) | AATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTAAACACA > minE/218101‑218234 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |