| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,818 | C→T | 6.1% | D162N (GAC→AAC) ‡ | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,818 | 0 | C | T | 6.1% | 388.9 / 8.5 | 115 | D162N (GAC→AAC) ‡ | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base C (62/46); new base T (3/4); total (65/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 120 total aligned reads displayed.
ACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTC > minE/1439750‑1439887 | aCTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTc < 1:3982698/69‑1 (MQ=255)aCTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa < 1:2590613/71‑1 (MQ=255) cTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc < 1:1435957/71‑1 (MQ=255) cTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc > 1:511295/1‑71 (MQ=255) tAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc > 1:3863923/1‑70 (MQ=255) aaGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGa > 1:814612/1‑69 (MQ=255) aaGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGAcc > 1:2521302/1‑71 (MQ=255) ccTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAcc > 1:3573862/1‑63 (MQ=255) ccTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAcc > 1:2042108/1‑63 (MQ=255) ccTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa < 1:939048/71‑1 (MQ=255) ccTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa < 1:4038060/71‑1 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa > 1:1057791/1‑57 (MQ=255) cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCaa > 1:3797908/1‑57 (MQ=255) aCCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt > 1:2897085/1‑71 (MQ=255) aCCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt < 1:2022812/71‑1 (MQ=255) aGATGGGTTGCCGTTTTTACCCTGGTACTGAACAAAAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAg < 1:1163407/71‑1 (MQ=255) tGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt > 1:856898/1‑71 (MQ=255) tGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt > 1:3487694/1‑71 (MQ=255) ggTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGtt < 1:1277748/70‑1 (MQ=255) gTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTAc > 1:2736074/1‑71 (MQ=255) ttGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa < 1:956409/49‑1 (MQ=255) cttcGTTGTTACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACg < 1:56719/67‑1 (MQ=2) gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:3717165/70‑1 (MQ=255) gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg > 1:3802273/1‑70 (MQ=255) gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:1424771/70‑1 (MQ=255) gTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaagaa > 1:3198704/1‑54 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAAc < 1:1887122/41‑1 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa < 1:1841386/63‑1 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg > 1:510827/1‑66 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGt > 1:3220468/1‑71 (MQ=255) ttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgt > 1:2695949/1‑58 (MQ=255) tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTc > 1:214731/1‑70 (MQ=255) attACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGttg > 1:3924542/2‑69 (MQ=255) ttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc < 1:2411618/71‑1 (MQ=9) ttACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgc < 1:3110226/71‑1 (MQ=255) ttACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgc < 1:1804202/71‑1 (MQ=255) ttACACTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgc > 1:1130816/1‑71 (MQ=255) tACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgc > 1:1937053/1‑70 (MQ=255) tACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgc > 1:3849331/1‑70 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt < 1:382139/55‑1 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa < 1:3749021/71‑1 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg < 1:3279985/71‑1 (MQ=255) cTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa > 1:2445327/1‑55 (MQ=255) cTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa > 1:1374860/1‑55 (MQ=255) cTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc < 1:2598011/71‑1 (MQ=255) tgatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGt > 1:3415493/4‑62 (MQ=2) tGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt > 1:3023380/1‑46 (MQ=255) tGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt > 1:3092619/1‑46 (MQ=255) ggTACTGAACAGCAAAGTTCAGGCCGTCAACCAGaa > 1:110833/1‑35 (MQ=255) gatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGa < 1:695181/64‑1 (MQ=255) gatACTGCACAGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGa > 1:3624457/3‑66 (MQ=9) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg > 1:3478811/1‑55 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGGCGCGAAGcc < 1:2959033/69‑1 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGAGTTACGGTAGGTCGCGAAGCCGt > 1:1755088/1‑71 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt < 1:835113/70‑1 (MQ=255) aCTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgt > 1:3979937/1‑46 (MQ=255) aCTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAg > 1:1692942/1‑45 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc < 1:3002901/71‑1 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc > 1:3807269/1‑71 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc > 1:1429344/1‑71 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc > 1:3251623/1‑71 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc < 1:3296023/71‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaat < 1:3753704/36‑2 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAg < 1:1233672/43‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:26319/51‑1 (MQ=255) tGAACAGCAAAGTTCAGCCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:2017753/51‑1 (MQ=255) gAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:1128244/50‑1 (MQ=255) gAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc > 1:539170/1‑63 (MQ=255) aaCAGCAAAGTTCAGGCCGTCACCCAGACCGAAGAAGTCAgtg > 1:1301783/1‑43 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtg > 1:33904/1‑43 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa > 1:896892/1‑46 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcg > 1:2840096/1‑56 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTa > 1:2715422/1‑67 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCa > 1:791053/1‑70 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCa < 1:3859286/70‑1 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAc > 1:3444189/1‑71 (MQ=255) aCAGCAAAGTTCAGGCCGTCAACCAGACCgaagaa > 1:2719652/1‑35 (MQ=255) cAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgc > 1:3674322/1‑71 (MQ=255) cAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgc > 1:3726392/1‑71 (MQ=255) gCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa > 1:795070/1‑42 (MQ=255) cAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc < 1:1241078/57‑1 (MQ=255) cAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctgc < 1:3762712/71‑1 (MQ=255) aaaGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTa > 1:3439588/1‑45 (MQ=255) aaaGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctgct > 1:2463292/1‑71 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGcc < 1:4078005/54‑1 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgct < 1:3670763/65‑1 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCa > 1:1941883/1‑71 (MQ=255) gTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACgat < 1:154663/41‑3 (MQ=12) ttCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:128481/39‑1 (MQ=255) ttCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTa > 1:455223/1‑41 (MQ=255) aGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGt < 1:81904/41‑1 (MQ=255) aGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGa < 1:3871500/70‑1 (MQ=255) aGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGa < 1:1720907/70‑1 (MQ=255) ccGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGtt < 1:3748706/71‑1 (MQ=255) cGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg < 1:1330184/71‑1 (MQ=255) tCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTc < 1:2383714/71‑1 (MQ=255) cAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc > 1:2060136/1‑42 (MQ=255) cAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGt > 1:1703648/1‑66 (MQ=255) cAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGt > 1:386393/1‑69 (MQ=255) cAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGAATGAAGt > 1:2824373/1‑66 (MQ=255) | ACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTC > minE/1439750‑1439887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |