| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,439,840 | G→A | 6.4% | N154N (AAC→AAT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,439,840 | 0 | G | A | 6.4% | 361.5 / 9.6 | 109 | N154N (AAC→AAT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (51/51); new base A (3/4); total (54/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
TGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCG > minE/1439771‑1439909 | tGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt > 1:856898/1‑71 (MQ=255) gggTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt > 1:1224135/1‑70 (MQ=255) gTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTAc > 1:2736074/1‑71 (MQ=255) gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg > 1:829369/1‑70 (MQ=255) gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:3717165/70‑1 (MQ=255) gCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:1424771/70‑1 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa < 1:1841386/63‑1 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg > 1:510827/1‑66 (MQ=255) tttttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGt > 1:3220468/1‑71 (MQ=255) tttACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTc > 1:214731/1‑70 (MQ=255) attACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGttg > 1:3924542/2‑69 (MQ=255) ttACCTTGATACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTcgc < 1:2411618/71‑1 (MQ=9) ttACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgc < 1:3110226/71‑1 (MQ=255) ttACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgc < 1:1804202/71‑1 (MQ=255) ttACACTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgc > 1:1130816/1‑71 (MQ=255) tACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcgc > 1:1937053/1‑70 (MQ=255) tACCCTGATATTGCAAAGCAAAGTTCAAGCCATCGACCTAACCAAAGAAGCCATTGTTACGGTAGGTtgc > 1:3849331/1‑70 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt < 1:382139/55‑1 (MQ=255) cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa < 1:3749021/71‑1 (MQ=255) ccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAg < 1:3279985/71‑1 (MQ=255) cTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa > 1:2445327/1‑55 (MQ=255) cTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc < 1:2598011/71‑1 (MQ=255) tgatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGt > 1:3415493/4‑62 (MQ=2) gatACTGCACCGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGa < 1:695181/64‑1 (MQ=255) gatACTGCACAGCGAAGTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACGATAAGTCGCGa > 1:3624457/3‑66 (MQ=9) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg > 1:3478811/1‑55 (MQ=255) gTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGAGTTACGGTAGGTCGCGAAGCCGt > 1:1755088/1‑71 (MQ=255) tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt < 1:835113/70‑1 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc > 1:1429344/1‑71 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc < 1:2004825/71‑1 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc > 1:3807269/1‑71 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc < 1:3002901/71‑1 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc < 1:1489981/71‑1 (MQ=255) cTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc > 1:3251623/1‑71 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:2347834/51‑1 (MQ=255) tGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:26319/51‑1 (MQ=255) tGAACAGCAAAGTTCAGCCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:2017753/51‑1 (MQ=255) gAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:1128244/50‑1 (MQ=255) gAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAg > 1:1760466/1‑53 (MQ=255) gAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAg > 1:391919/1‑53 (MQ=255) gAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc > 1:539170/1‑63 (MQ=255) aaCAGCAAAGTTCAGGCCGTCACCCAGACCGAAGAAGTCAgtg > 1:1301783/1‑43 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtg > 1:33904/1‑43 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa > 1:896892/1‑46 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTcg > 1:2840096/1‑56 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTa > 1:2715422/1‑67 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCa < 1:610035/70‑1 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCa > 1:791053/1‑70 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAc > 1:1326101/1‑71 (MQ=255) aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCAc > 1:3444189/1‑71 (MQ=255) cAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgc > 1:3726392/1‑71 (MQ=255) cAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgc > 1:3674322/1‑71 (MQ=255) gCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTa > 1:795070/1‑42 (MQ=255) cAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc < 1:1241078/57‑1 (MQ=255) cAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctgc < 1:3762712/71‑1 (MQ=255) aaaGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTa > 1:3439588/1‑45 (MQ=255) aaaGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctgct > 1:2463292/1‑71 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgct < 1:3670763/65‑1 (MQ=255) gTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCa > 1:1941883/1‑71 (MQ=255) gTTCAGACCATTTACCAGACCGAAGAAATCAGTATTACgat < 1:154663/41‑3 (MQ=12) ttCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:128481/39‑1 (MQ=255) ttCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg < 1:3844695/39‑1 (MQ=255) ttCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTa > 1:455223/1‑41 (MQ=255) aGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGa < 1:1720907/70‑1 (MQ=255) aGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGa < 1:3871500/70‑1 (MQ=255) ccGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGtt < 1:3748706/71‑1 (MQ=255) cGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg < 1:1330184/71‑1 (MQ=255) cAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGt > 1:386393/1‑69 (MQ=255) cAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGAATGAAGt > 1:2824373/1‑66 (MQ=255) aaCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc > 1:539071/1‑47 (MQ=255) aaCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTAc > 1:2956460/1‑47 (MQ=255) aaCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGt > 1:1665230/1‑68 (MQ=255) aaCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGt > 1:769298/1‑68 (MQ=255) aCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg < 1:1256100/66‑1 (MQ=255) aCCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg < 1:4121975/66‑1 (MQ=255) ccAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGc < 1:2412388/39‑1 (MQ=255) ccAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGaa > 1:3659485/1‑71 (MQ=255) cAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgc > 1:89399/1‑49 (MQ=255) aGACCAAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAAcc < 1:3266978/71‑1 (MQ=255) gACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCg < 1:1317516/71‑1 (MQ=255) aCCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGa < 1:1721161/66‑1 (MQ=255) ccGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATg < 1:2215141/54‑1 (MQ=255) ccGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTa < 1:1052370/71‑1 (MQ=255) gaagaaGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAg < 1:2306325/70‑1 (MQ=255) aagaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTa > 1:2622520/1‑36 (MQ=255) aagaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgt < 1:246267/71‑1 (MQ=255) agaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCa > 1:2705833/1‑59 (MQ=255) agaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCg > 1:3081415/1‑65 (MQ=255) agaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgtg < 1:3156836/71‑1 (MQ=255) gaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgtgt > 1:293570/1‑71 (MQ=255) aagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTc < 1:201222/71‑1 (MQ=255) aagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTc < 1:2610395/71‑1 (MQ=255) gTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcac < 1:330694/71‑1 (MQ=255) gTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcac < 1:3990502/71‑1 (MQ=255) aGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGaa > 1:3545376/1‑45 (MQ=255) aGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcaccac > 1:3239742/1‑71 (MQ=255) gtgtTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCaccacc < 1:4039050/71‑1 (MQ=255) tgtTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgt < 1:2242291/61‑1 (MQ=255) tgtTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCaccacc > 1:3067552/1‑70 (MQ=255) tgtTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCg < 1:3804489/71‑1 (MQ=255) | TGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCG > minE/1439771‑1439909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |