Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,440,224 G→A 10.0% N26N (AAC→AAT ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,440,2240GA10.0% 275.8 / 14.7 90N26N (AAC→AATompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (49/32);  new base A (4/5);  total (53/37)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 102 total aligned reads displayed.

ATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTA  >  minE/1440155‑1440293
                                                                     |                                                                     
aTCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgt                                                                      >  1:3808206/1‑71 (MQ=255)
  cTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgttg                                                                    >  1:1207802/1‑71 (MQ=255)
  cTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgttg                                                                    >  1:526457/1‑71 (MQ=255)
  cTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgtt                                                                     >  1:190007/1‑70 (MQ=255)
   tttGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgttg                                                                    >  1:3164376/1‑70 (MQ=255)
    ttgttgTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgttg                                                                    >  1:901331/1‑69 (MQ=255)
    ttgttgTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTAGCCGTCTTtgttgt                                                                   >  1:3180531/1‑70 (MQ=255)
     tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa                                                                  >  1:286402/1‑70 (MQ=255)
     tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa                                                                  <  1:3437186/70‑1 (MQ=255)
      gttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGaaa                                                                 <  1:674239/70‑1 (MQ=255)
       ttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa                                                                  <  1:1618476/68‑1 (MQ=255)
       ttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAAc                                                               <  1:352849/71‑1 (MQ=255)
          tCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt                                                             <  1:792642/70‑1 (MQ=255)
          tCAGAAAAATAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTc                                                            <  1:531551/71‑1 (MQ=14)
             gagaAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa                                                                  <  1:4081901/62‑1 (MQ=255)
                aaaTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCagcagc                                                      >  1:3032291/1‑71 (MQ=255)
                aaaTAATGCAGGCCATCAACTTTGCCATACAGCTCTAATTTGTTGCCGTCTttatt                                                                     >  1:175095/1‑56 (MQ=9)
                  aTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaaa                                                                >  1:874048/1‑59 (MQ=255)
                  aTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaaa                                                                >  1:2422961/1‑59 (MQ=255)
                  aTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaa                                                                 >  1:2449318/1‑58 (MQ=255)
                  aTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGt                                                    >  1:1576358/1‑71 (MQ=255)
                     gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg                                                 <  1:3793346/71‑1 (MQ=255)
                         aGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc                                             <  1:3771150/71‑1 (MQ=255)
                         aGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcag                                              >  1:233014/1‑68 (MQ=12)
                             cGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCt                                         >  1:3326044/1‑71 (MQ=255)
                             cGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCt                                         >  1:593970/1‑71 (MQ=255)
                             cGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCt                                         >  1:3762868/1‑71 (MQ=255)
                             cGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCt                                         <  1:1553432/71‑1 (MQ=255)
                              gTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCg                                                     <  1:3423355/58‑1 (MQ=255)
                               tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgct                                               <  1:322023/63‑1 (MQ=255)
                               tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgct                                               <  1:814031/63‑1 (MQ=255)
                               tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGc                                       <  1:4104165/71‑1 (MQ=255)
                               acTACTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGCCAGc                                       <  1:2431350/70‑1 (MQ=2)
                                caaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGcg                                      >  1:3655588/3‑70 (MQ=12)
                                 tACTTTACCGTACAGATCTAATTTGTTGCCGTCTttgtt                                                                     <  1:3268080/39‑1 (MQ=255)
                                 tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaa                                                                 <  1:189016/43‑1 (MQ=255)
                                 tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaa                                                                 <  1:1848567/43‑1 (MQ=255)
                                 tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCt                                      >  1:794748/1‑70 (MQ=255)
                                 tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCt                                      >  1:2471053/1‑70 (MQ=255)
                                 tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa                                     >  1:3331113/1‑71 (MQ=255)
                                 tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa                                     >  1:3587972/1‑71 (MQ=255)
                                 tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCCCCTGCTa                                     >  1:939324/1‑71 (MQ=255)
                                   cTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa                                     <  1:3433959/69‑1 (MQ=255)
                                   cTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTAc                                    >  1:1208978/1‑70 (MQ=255)
                                    tttACCGTACAGATCTAATTTGTTGCTGTCTTTGTTCTAAACTTc                                                            <  1:289966/45‑1 (MQ=18)
                                    tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt                                                  >  1:1812468/1‑55 (MQ=255)
                                     ttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc                                             >  1:1136594/1‑59 (MQ=255)
                                     ttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                 >  1:3663847/1‑71 (MQ=255)
                                     ttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                 >  1:2607038/1‑71 (MQ=255)
                                      tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTTTa                                                                  >  1:1555737/1‑37 (MQ=255)
                                      tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaaa                                                                >  1:920854/1‑39 (MQ=255)
                                      tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa                                                                  >  1:2660141/1‑37 (MQ=255)
                                      tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt                                                             >  1:2033293/1‑42 (MQ=255)
                                      tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt                                                             >  1:2305047/1‑42 (MQ=255)
                                      tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTAcc                                   >  1:132367/1‑68 (MQ=255)
                                      tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                 >  1:3788287/1‑70 (MQ=255)
                                      tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                 >  1:636137/1‑70 (MQ=255)
                                      tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                 >  1:858912/1‑70 (MQ=255)
                                      tACCGTACAGATCTAATTTGTCGCCGTCTTTGTTGTAAACtt                                                             >  1:2252344/1‑42 (MQ=255)
                                       aCCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaaa                                                                >  1:765147/1‑38 (MQ=255)
                                        ccGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagcag                              <  1:432744/71‑1 (MQ=255)
                                        ccGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagcag                              <  1:2371333/71‑1 (MQ=255)
                                         cGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCaga                             <  1:1056624/71‑1 (MQ=255)
                                         cGTACAAATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCaga                             <  1:3328512/71‑1 (MQ=255)
                                          gTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCagag                            <  1:2666157/71‑1 (MQ=255)
                                          atACAGATCTAATTTGTTGCCGGCTTTATTATAAACTTCGGCTGCATGtgca                                               <  1:1315586/51‑2 (MQ=255)
                                           tACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCggctgca                                                     <  1:2687171/45‑2 (MQ=25)
                                           tACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcaggg                            >  1:3501576/1‑68 (MQ=255)
                                             cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCt                          <  1:1738901/70‑1 (MQ=255)
                                              aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagca                               <  1:3897543/64‑1 (MQ=255)
                                              aGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcagg                        <  1:709534/71‑1 (MQ=255)
                                                aTCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcaggaa                      >  1:3958810/1‑69 (MQ=255)
                                                  cTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCagag                            >  1:1837368/1‑63 (MQ=255)
                                                  cTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCagag                            >  1:4043625/1‑63 (MQ=255)
                                                  cTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc                     >  1:1211838/1‑70 (MQ=255)
                                                  cTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc                     >  1:1722421/1‑70 (MQ=255)
                                                   tAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagcag                              <  1:271597/60‑1 (MQ=255)
                                                    aaTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg                        >  1:891821/1‑65 (MQ=255)
                                                     aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgc                                                >  1:37051/1‑40 (MQ=255)
                                                     aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagg                 <  1:2844197/71‑1 (MQ=255)
                                                     aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                  >  1:3850430/1‑70 (MQ=255)
                                                     aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                  >  1:1635220/1‑70 (MQ=255)
                                                     aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                  <  1:1220555/70‑1 (MQ=255)
                                                     aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                  <  1:3001916/70‑1 (MQ=255)
                                                      tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctg                                              >  1:2487293/1‑41 (MQ=255)
                                                      tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga                <  1:3297991/71‑1 (MQ=255)
                                                      tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                  >  1:1221244/1‑69 (MQ=255)
                                                      tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                  >  1:3458204/1‑69 (MQ=255)
                                                        tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgc                                                >  1:1305596/1‑37 (MQ=255)
                                                        tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                  >  1:2807663/1‑67 (MQ=255)
                                                          ttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGa            <  1:2438223/71‑1 (MQ=255)
                                                             ccGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAg         <  1:3334533/71‑1 (MQ=255)
                                                                tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCt                                         <  1:1823416/36‑1 (MQ=255)
                                                                  tttGTTGTAAACTTGAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga                >  1:1808216/1‑59 (MQ=255)
                                                                  tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCaga                             >  1:1210149/1‑46 (MQ=255)
                                                                  tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCATAGCTGGGACCagga                >  1:590274/1‑59 (MQ=255)
                                                                  tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt    >  1:260650/1‑71 (MQ=255)
                                                                    tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTa       >  1:3604781/1‑66 (MQ=255)
                                                                    tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt   <  1:2193015/70‑1 (MQ=255)
                                                                     gttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTa  <  1:3488606/70‑1 (MQ=255)
                                                                     |                                                                     
ATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTA  >  minE/1440155‑1440293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: