Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,440,242 G→A 8.0% N20N (AAC→AAT) ‡ ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,440,2420GA 8.0% 279.7 / 10.3 87N20N (AAC→AAT) ‡ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (43/37);  new base A (4/3);  total (47/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

ATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAC  >  minE/1440173‑1440310
                                                                     |                                                                    
aTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGt                                                                     >  1:1576358/1‑71 (MQ=255)
   gTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg                                                                  <  1:3793346/71‑1 (MQ=255)
       aGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc                                                              <  1:3771150/71‑1 (MQ=255)
       aGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcag                                                               >  1:233014/1‑68 (MQ=12)
           cGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCt                                                          >  1:3762868/1‑71 (MQ=255)
           cGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCt                                                          >  1:3326044/1‑71 (MQ=255)
           cGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCt                                                          >  1:593970/1‑71 (MQ=255)
           cGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCt                                                          <  1:1553432/71‑1 (MQ=255)
            gTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCg                                                                      <  1:3423355/58‑1 (MQ=255)
             tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgct                                                                <  1:322023/63‑1 (MQ=255)
             tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgct                                                                <  1:814031/63‑1 (MQ=255)
             tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGc                                                        <  1:4104165/71‑1 (MQ=255)
             acTACTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGCCAGc                                                        <  1:2431350/70‑1 (MQ=2)
              caaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGcg                                                       >  1:3655588/3‑70 (MQ=12)
               tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCt                                                       >  1:2471053/1‑70 (MQ=255)
               tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCt                                                       >  1:794748/1‑70 (MQ=255)
               tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa                                                      >  1:3587972/1‑71 (MQ=255)
               tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa                                                      >  1:3331113/1‑71 (MQ=255)
               tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCCCCTGCTa                                                      >  1:939324/1‑71 (MQ=255)
                 cTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgc                                                                 >  1:980708/1‑58 (MQ=255)
                 cTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa                                                      <  1:3433959/69‑1 (MQ=255)
                 cTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTAc                                                     >  1:1208978/1‑70 (MQ=255)
                  tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt                                                                   >  1:1812468/1‑55 (MQ=255)
                   ttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc                                                              >  1:1136594/1‑59 (MQ=255)
                   ttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                                  >  1:2607038/1‑71 (MQ=255)
                   ttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                                  >  1:3663847/1‑71 (MQ=255)
                    tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTAcc                                                    >  1:132367/1‑68 (MQ=255)
                    tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                                  >  1:636137/1‑70 (MQ=255)
                    tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                                  >  1:3788287/1‑70 (MQ=255)
                    tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag                                                  >  1:858912/1‑70 (MQ=255)
                      ccGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagcag                                               <  1:432744/71‑1 (MQ=255)
                      ccGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagcag                                               <  1:2371333/71‑1 (MQ=255)
                       cGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCaga                                              <  1:1056624/71‑1 (MQ=255)
                       cGTACAAATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCaga                                              <  1:3328512/71‑1 (MQ=255)
                        gTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCagag                                             <  1:2666157/71‑1 (MQ=255)
                        atACAGATCTAATTTGTTGCCGGCTTTATTATAAACTTCGGCTGCATGtgca                                                                <  1:1315586/51‑2 (MQ=255)
                         tACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcaggg                                             >  1:3501576/1‑68 (MQ=255)
                           cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCt                                           <  1:1738901/70‑1 (MQ=255)
                            aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagca                                                <  1:3897543/64‑1 (MQ=255)
                            aGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcagg                                         <  1:709534/71‑1 (MQ=255)
                              aTCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcaggaa                                       >  1:3958810/1‑69 (MQ=255)
                                cTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCagag                                             >  1:1837368/1‑63 (MQ=255)
                                cTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCagag                                             >  1:4043625/1‑63 (MQ=255)
                                cTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc                                      >  1:1722421/1‑70 (MQ=255)
                                cTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc                                      >  1:1211838/1‑70 (MQ=255)
                                 tAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcagcag                                               <  1:271597/60‑1 (MQ=255)
                                  aaTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg                                         >  1:891821/1‑65 (MQ=255)
                                   aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgc                                                                 >  1:37051/1‑40 (MQ=255)
                                   aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagg                                  <  1:2844197/71‑1 (MQ=255)
                                   aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                                   >  1:3850430/1‑70 (MQ=255)
                                   aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                                   <  1:3001916/70‑1 (MQ=255)
                                   aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                                   <  1:1220555/70‑1 (MQ=255)
                                   aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                                   >  1:1635220/1‑70 (MQ=255)
                                    tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctg                                                               >  1:2487293/1‑41 (MQ=255)
                                    tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga                                 <  1:3297991/71‑1 (MQ=255)
                                    tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                                   >  1:3458204/1‑69 (MQ=255)
                                    tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                                   >  1:1221244/1‑69 (MQ=255)
                                      tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgc                                                                 >  1:1305596/1‑37 (MQ=255)
                                      tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag                                   >  1:2807663/1‑67 (MQ=255)
                                        ttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGa                             <  1:2438223/71‑1 (MQ=255)
                                           ccGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAg                          <  1:3334533/71‑1 (MQ=255)
                                              tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCt                                                          <  1:1823416/36‑1 (MQ=255)
                                                tttGTTGTAAACTTGAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga                                 >  1:1808216/1‑59 (MQ=255)
                                                tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCaga                                              >  1:1210149/1‑46 (MQ=255)
                                                tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCATAGCTGGGACCagga                                 >  1:590274/1‑59 (MQ=255)
                                                tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt                     >  1:260650/1‑71 (MQ=255)
                                                  tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTa                        >  1:3604781/1‑66 (MQ=255)
                                                  tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt                    <  1:2193015/70‑1 (MQ=255)
                                                   gttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTa                   <  1:3488606/70‑1 (MQ=255)
                                                    ttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTaa                  <  1:1011212/70‑1 (MQ=255)
                                                     tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACt                >  1:1116879/1‑71 (MQ=255)
                                                       tAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACt                      <  1:9456/63‑1 (MQ=255)
                                                       tAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACtt               <  1:3962571/70‑1 (MQ=255)
                                                         aaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTc             <  1:3179039/70‑1 (MQ=255)
                                                         aaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTc             <  1:2343046/70‑1 (MQ=255)
                                                         aaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTc             <  1:2702851/70‑1 (MQ=255)
                                                         aaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCa            <  1:3863909/71‑1 (MQ=255)
                                                         aaCTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCa            >  1:683238/1‑71 (MQ=255)
                                                           cTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGa                                       >  1:2886290/1‑42 (MQ=255)
                                                              cagcagCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGtta       >  1:1469422/1‑71 (MQ=255)
                                                              cagcagCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGtta       >  1:2564176/1‑71 (MQ=255)
                                                              cagcagCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGtt        <  1:2795904/70‑1 (MQ=255)
                                                              cagcagCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACATTAACTTTCATGtt        <  1:19316/70‑1 (MQ=255)
                                                               agcagcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACttt              <  1:1659941/63‑1 (MQ=255)
                                                               agcagcGGTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGttat      <  1:4081949/71‑1 (MQ=255)
                                                                ggagcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGttttt     <  1:3133954/69‑1 (MQ=255)
                                                                gcagcGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGttat      >  1:1691405/1‑70 (MQ=255)
                                                                    cGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGa                             <  1:989697/43‑1 (MQ=255)
                                                                     gTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGttatta    >  1:1514106/1‑67 (MQ=255)
                                                                     gTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAc  >  1:3586559/1‑69 (MQ=255)
                                                                     |                                                                    
ATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAAC  >  minE/1440173‑1440310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: