| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 219,287 | G→A | 5.2% | E41K (GAA→AAA) | ivy → | inhibitor of vertebrate C‑lysozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 219,287 | 0 | G | A | 5.2% | 312.0 / 5.1 | 97 | E41K (GAA→AAA) | ivy | inhibitor of vertebrate C‑lysozyme |
| Reads supporting (aligned to +/- strand): ref base G (59/31); new base A (2/3); total (63/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GCCGCTCTGGTCATCGCCACCAGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGTGATGAAAGGCGG > minE/219218‑219357 | gccgcTCTGGTCATCGCCACCAGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCg > 1:3426943/1‑70 (MQ=255)gccgcTCTGGTCATCGCCACCAGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCg > 1:1241034/1‑70 (MQ=255) ccgcTCTGGTCATCGCCACCAGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGaa < 1:3863988/71‑1 (MQ=255) ctctGGTCATCGCCACCAGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAacc < 1:146681/71‑1 (MQ=255) cATCGCCACCAGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAg > 1:2197563/1‑71 (MQ=255) aTCGCCACCAGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGc > 1:2766782/1‑71 (MQ=255) aTCGCCACCAGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCAAAACCACCAAAGc > 1:2258834/1‑71 (MQ=255) tCGCCACCAGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGc < 1:3218192/70‑1 (MQ=255) gCCACCAGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTg > 1:1525711/1‑70 (MQ=255) acaGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCAttt < 1:3107246/70‑1 (MQ=255) caGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTa < 1:2921729/71‑1 (MQ=255) caGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCAAAACCACCAAAGCTGCATTTa < 1:1772975/71‑1 (MQ=255) gTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTaa < 1:855139/70‑1 (MQ=255) gTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAAt > 1:3439323/1‑71 (MQ=255) cAATGGCGCAGGATGATTTATCCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAg < 1:2923005/71‑1 (MQ=255) cAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAAt > 1:2553555/1‑68 (MQ=255) cAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAAt > 1:1404319/1‑68 (MQ=255) cAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCa < 1:2957105/70‑1 (MQ=255) cAATGGCGCACGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGc > 1:1253775/1‑61 (MQ=255) aTGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGAt > 1:3343001/1‑71 (MQ=255) tGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTa < 1:1848856/63‑1 (MQ=255) tGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATg > 1:260425/1‑71 (MQ=255) ggCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCTAAACCACCAAAGCTGCATTTAATCAGATgg > 1:2961580/1‑71 (MQ=255) ggCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATgg > 1:2916240/1‑71 (MQ=255) gcgcAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGc > 1:2706765/1‑56 (MQ=255) cgcAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAg > 1:419819/1‑51 (MQ=255) cgcAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATgg < 1:4052397/69‑1 (MQ=255) gcAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTa > 1:591345/1‑70 (MQ=255) cAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACa < 1:1113424/71‑1 (MQ=255) aGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTAc > 1:1705458/1‑69 (MQ=255) tatgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCaa < 1:320448/43‑1 (MQ=255) gatgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTaa > 1:2589117/1‑56 (MQ=255) gatgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTaa > 1:2584175/1‑56 (MQ=255) gatgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTaa > 1:2357068/1‑56 (MQ=255) gatgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTa < 1:1144572/55‑1 (MQ=255) gatgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTAc < 1:1208260/67‑1 (MQ=255) atgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGc < 1:1066398/49‑1 (MQ=255) tgatTTAACCATTAGCAGCCTTGCAAAGGTCGAAACCACCAAAGCTGCATTTAATCAGAt < 1:1394363/60‑1 (MQ=255) tgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAacca > 1:451090/1‑38 (MQ=255) tgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAAt > 1:1638491/1‑55 (MQ=255) tgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACaa > 1:2352150/1‑67 (MQ=255) tgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACaa > 1:3316423/1‑67 (MQ=255) tgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACaa > 1:882559/1‑67 (MQ=255) tgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAggg > 1:2170133/1‑70 (MQ=255) tgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAggg > 1:1375098/1‑70 (MQ=255) tgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAggg > 1:1838266/1‑70 (MQ=255) tgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAggg > 1:528870/1‑70 (MQ=255) tgatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGc < 1:3351086/71‑1 (MQ=255) tgatTAAACCATTAGCAGCCTTGCAAAGGGCCAAacc > 1:3633873/1‑37 (MQ=38) gatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCa < 1:1404202/56‑1 (MQ=255) gatTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCa > 1:3887712/1‑71 (MQ=255) atTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCAt < 1:535881/71‑1 (MQ=255) tttAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCAt > 1:2705607/1‑70 (MQ=255) ttAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCAt > 1:3975385/1‑69 (MQ=255) aCCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGc < 1:3421482/70‑1 (MQ=255) ccATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGctg > 1:1597184/1‑71 (MQ=255) cATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATaa < 1:3233345/66‑1 (MQ=255) ttAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAg < 1:2020671/47‑1 (MQ=255) tAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGctgcctg > 1:1233217/1‑71 (MQ=255) agcagcCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGctgcctgc > 1:2102721/1‑71 (MQ=255) agcagcCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGctgcctg > 1:3843011/1‑70 (MQ=255) gcagcCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGctgcctgc < 1:3153756/70‑1 (MQ=255) agcCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAAt > 1:3336411/1‑39 (MQ=255) agcCTTGCAAAGGGCAAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTgcctgcct < 1:3171465/70‑1 (MQ=255) gcCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTgg < 1:109592/71‑1 (MQ=255) gcCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTgg < 1:503536/71‑1 (MQ=255) ccTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCa < 1:2059686/39‑1 (MQ=255) ccTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCa < 1:2353255/39‑1 (MQ=255) cTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGt > 1:713927/1‑71 (MQ=255) cTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGt > 1:2619488/1‑71 (MQ=255) cTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGt > 1:1724179/1‑71 (MQ=255) cTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGt > 1:2088174/1‑71 (MQ=255) ttGCAAAGGGCGAAACCACCAAAGCTGCATTTAAt > 1:3383368/1‑35 (MQ=255) ttGCAAAGGGCGAAACCACCAAAGCTGCATTTAAt > 1:2655535/1‑35 (MQ=255) ttGCAAAGGGCGAAACCACCAAAGCTGCATTTAAt > 1:1066897/1‑35 (MQ=255) ttGCAAAGGGCGAAACCACCAAAGCTGCATTTAAt > 1:1295949/1‑35 (MQ=255) ttGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATgg < 1:778787/42‑1 (MQ=255) ttGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGt > 1:1252561/1‑70 (MQ=255) ttGCAAAGGGCAAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTgcctgcct < 1:3469223/66‑1 (MQ=255) tGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGc > 1:3207416/1‑56 (MQ=255) tGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGc > 1:2520757/1‑56 (MQ=255) gCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAg > 1:2533551/1‑36 (MQ=255) gCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGAt > 1:1021744/1‑38 (MQ=255) gCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTAc < 1:2314067/43‑1 (MQ=255) gCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGc > 1:2510266/1‑55 (MQ=255) gCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGc > 1:2037755/1‑55 (MQ=255) gCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGtgat > 1:2488599/1‑71 (MQ=255) aGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACaa > 1:3921421/1‑41 (MQ=255) aGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGtgatg > 1:3373117/1‑68 (MQ=255) ggCAAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGCGCATAAGctg > 1:1678763/1‑51 (MQ=255) gCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAg > 1:3692395/1‑39 (MQ=255) gCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGctgc > 1:828525/1‑51 (MQ=255) gCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGctgc > 1:1130343/1‑51 (MQ=255) gCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGTGATGAAAGGc < 1:452723/71‑1 (MQ=255) gCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGTGATGAAAGGc < 1:2042199/71‑1 (MQ=255) cGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCa > 1:2394360/1‑42 (MQ=255) cGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGTGATGAAAGGCg > 1:3123829/1‑71 (MQ=255) cGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGTGATGAAAGGCg > 1:1700763/1‑71 (MQ=255) gAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGTGATGAAAGGCgg < 1:3639874/71‑1 (MQ=255) gAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGTGATGAAAGGCgg > 1:906997/1‑71 (MQ=255) | GCCGCTCTGGTCATCGCCACCAGTGCAATGGCGCAGGATGATTTAACCATTAGCAGCCTTGCAAAGGGCGAAACCACCAAAGCTGCATTTAATCAGATGGTACAAGGGCATAAGCTGCCTGCCTGGGTGATGAAAGGCGG > minE/219218‑219357 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |