| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,486,279 | T→C | 16.7% | T345A (ACA→GCA) | nuoN ← | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,486,279 | 0 | T | C | 16.7% | 139.9 / 21.8 | 60 | T345A (ACA→GCA) | nuoN | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Reads supporting (aligned to +/- strand): ref base T (29/21); new base C (6/4); total (35/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01 | |||||||||||
GCGCAGGTAGTAGTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGA > minE/1486211‑1486348 | gcgcAGGTAGTAGTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCt > 1:2129947/1‑71 (MQ=255) gtagAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACaccgaccg > 1:2914931/1‑71 (MQ=255) gtagAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACaccgacc < 1:1228133/70‑1 (MQ=255) gtagAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACaccgacc < 1:2823627/70‑1 (MQ=255) gagGCCGATTGCCGAACCGACAACCACGGCACCAACCAGCCACCACAAGTGTGTCTGGACACCGACCGc > 1:627803/1‑69 (MQ=255) agGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAg < 1:2666978/71‑1 (MQ=255) gCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCa > 1:529771/1‑71 (MQ=255) gCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCa < 1:3934344/71‑1 (MQ=255) ttGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACg < 1:226868/68‑1 (MQ=255) tGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAgtgtgt > 1:1826097/1‑45 (MQ=255) gCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGaa > 1:1774344/1‑71 (MQ=255) ccGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGa < 1:635509/48‑1 (MQ=255) ccGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGa < 1:3543831/48‑1 (MQ=255) ccGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGaa < 1:3376915/70‑1 (MQ=255) gAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGaa < 1:3094482/68‑1 (MQ=255) aaCCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTa < 1:2536138/71‑1 (MQ=255) ccGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCt > 1:908416/1‑40 (MQ=255) ccGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACacc > 1:563275/1‑47 (MQ=255) gACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTa > 1:280294/1‑67 (MQ=255) gACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTa > 1:932091/1‑67 (MQ=255) gACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTAc > 1:975564/1‑68 (MQ=255) aaCCACGGCACCCACCAGCCACCACAAGTGTGCCCGGACACCGACCGCCAGCACGTAGaa > 1:813647/1‑60 (MQ=255) aCGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGcc > 1:18005/1‑71 (MQ=255) aCGGCACCCACCAGCCACCACAAGTGTGCCTGTACACCGACCGCCAGCACGTAGAACTTACCGATAAAGcc > 1:724591/1‑71 (MQ=255) cGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGccc < 1:3934108/71‑1 (MQ=255) ggCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAg > 1:718910/1‑45 (MQ=255) ggCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAg > 1:688770/1‑45 (MQ=255) ggCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAg > 1:3379106/1‑45 (MQ=255) ggCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCa < 1:721180/71‑1 (MQ=255) cacccaccAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGc < 1:2848745/71‑1 (MQ=255) ccaccaGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTc < 1:3887576/71‑1 (MQ=255) accaGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCAt > 1:1683474/1‑71 (MQ=255) ccaGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATc > 1:3293166/1‑71 (MQ=255) ccaGCCACAACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATa > 1:4107380/1‑56 (MQ=255) caGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCg > 1:355469/1‑71 (MQ=255) aGCCACCACAAGTGTGTCTGGACACCGCCCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg > 1:1335971/1‑71 (MQ=255) aGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCAc > 1:604865/1‑38 (MQ=255) aGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:153363/71‑1 (MQ=255) aGCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGaa > 1:1121583/1‑44 (MQ=255) gCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg > 1:3169380/1‑70 (MQ=255) gCCACCACAAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg > 1:511478/1‑70 (MQ=255) ccaccaCAAGTGTGTCTGGACACCGACCGCCAGCACGTAGa < 1:1115956/41‑1 (MQ=255) caccacAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGAt < 1:2407402/71‑1 (MQ=255) accacAAGTGTGTCTGGACACCGACCGCCAGCACGTAg < 1:2508730/38‑1 (MQ=255) accacAAGTGTGTCTGGACACCGACCGCCAGCACGTAg < 1:787526/38‑1 (MQ=255) accacAAGTGTGTCTGGACACCGACCGCCAGCACGTAg < 1:1040198/38‑1 (MQ=255) accacAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATa > 1:3637652/1‑71 (MQ=255) accacAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATa > 1:746673/1‑71 (MQ=255) accacAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATa < 1:2156619/71‑1 (MQ=255) ccacAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGc < 1:648560/58‑1 (MQ=255) ccacAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATAc < 1:423920/71‑1 (MQ=255) cacaAGTGTGTCTGGACACCGACCGCCAGCACGTAGaa > 1:3953375/1‑38 (MQ=255) cacaAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATaa > 1:220062/1‑49 (MQ=255) cacaAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGc < 1:1865452/52‑1 (MQ=255) acaAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTa > 1:3876628/1‑41 (MQ=255) acaAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTa > 1:2942743/1‑41 (MQ=255) acaAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATa > 1:364834/1‑47 (MQ=255) acaAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATa > 1:2712358/1‑47 (MQ=255) acaAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCgg < 1:1064423/64‑1 (MQ=255) acaAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCg > 1:1762521/1‑71 (MQ=255) caAGTGTGCCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCgg > 1:1550347/1‑71 (MQ=255) gtgtgtCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGcc < 1:3780797/70‑1 (MQ=255) tgtgtCTGGACACCGACCGCCAGCACGTAGAACTTACCGATaaa > 1:3021954/1‑44 (MQ=255) tgtCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCaga < 1:2801392/70‑1 (MQ=255) tgtCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCaga < 1:2129906/70‑1 (MQ=255) gtCTGGACACCGACCGCCAGCACGTAGAACTTACCGATa < 1:259287/39‑1 (MQ=255) tCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCg > 1:2462141/1‑54 (MQ=255) tCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAgaga < 1:1358225/70‑1 (MQ=255) | GCGCAGGTAGTAGTAGAGGCCGATTGCCGAACCGACAACCACGGCACCCACCAGCCACCACAAGTGTGTCTGGACACCGACCGCCAGCACGTAGAACTTACCGATAAAGCCCAGCGTCATCGGGATACCGGCCAGAGA > minE/1486211‑1486348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |