| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 222,988 | T→G | 15.0% | intergenic (+43/‑137) | lpcA → / → prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 222,988 | 0 | T | G | 15.0% | 186.6 / 33.4 | 80 | intergenic (+43/‑137) | lpcA/prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Reads supporting (aligned to +/- strand): ref base T (30/38); new base G (4/8); total (34/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGG > minE/222919‑223058 | ttgattgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAtt < 1:3093897/71‑1 (MQ=255)ttgattgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAtt > 1:2127581/1‑71 (MQ=255)ttgattgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAtt < 1:3735513/71‑1 (MQ=255)ttgattgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAtt < 1:3834809/71‑1 (MQ=255)ttgattgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAtt > 1:1733439/1‑71 (MQ=255) gattgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAt < 1:1617161/68‑1 (MQ=255) gattgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTtg > 1:1142411/1‑71 (MQ=255) gattgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTtg > 1:3933237/1‑71 (MQ=255) gattgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTtg > 1:421712/1‑71 (MQ=255) ttgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAt < 1:1052485/66‑1 (MQ=255) ttgaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTtgtg > 1:3354382/1‑71 (MQ=255) gaAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcg > 1:67990/1‑71 (MQ=255) aaaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcg < 1:544005/70‑1 (MQ=255) aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAttt > 1:1431471/1‑64 (MQ=255) aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:2110079/71‑1 (MQ=255) aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:2535133/71‑1 (MQ=255) aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:3471632/71‑1 (MQ=255) aaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctg < 1:298310/71‑1 (MQ=255) aaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct > 1:1579794/1‑70 (MQ=255) agagATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgat > 1:1190847/1‑71 (MQ=255) aTGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct < 1:404501/71‑1 (MQ=255) aTGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct > 1:3590817/1‑71 (MQ=255) tGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTtgt < 1:2990156/58‑1 (MQ=255) tGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTtgt < 1:2221802/58‑1 (MQ=255) ggTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatg > 1:510023/1‑66 (MQ=255) ggTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatg > 1:421964/1‑66 (MQ=255) ttAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatg > 1:430694/1‑71 (MQ=255) ttAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatg > 1:1424715/1‑71 (MQ=255) ttAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatg > 1:2509624/1‑71 (MQ=255) agtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct < 1:1477924/64‑1 (MQ=255) agtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCg < 1:2909023/70‑1 (MQ=255) agtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCg < 1:389116/70‑1 (MQ=255) gtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatg < 1:2619229/60‑1 (MQ=255) gtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:3301149/56‑1 (MQ=255) gtaagtCTGGCCTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:1945690/56‑1 (MQ=255) taagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTgcg < 1:1414670/53‑1 (MQ=255) aagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct < 1:3887419/61‑1 (MQ=255) agtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCAtt > 1:1508497/1‑45 (MQ=255) agtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgc < 1:4090573/58‑1 (MQ=255) tGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcg > 1:3618317/1‑71 (MQ=255) tGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcg > 1:3623050/1‑71 (MQ=255) gCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGa > 1:1322626/1‑67 (MQ=255) gCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcgc < 1:413923/70‑1 (MQ=255) gCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAc < 1:3192193/68‑1 (MQ=255) tgTAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCt < 1:2882678/61‑1 (MQ=9) cGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGt > 1:238771/1‑71 (MQ=14) cGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:1250631/46‑1 (MQ=255) gTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:2129690/45‑1 (MQ=255) gTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTc < 1:1687108/71‑1 (MQ=255) tAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTa > 1:2702424/1‑71 (MQ=12) tAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTa < 1:2568575/71‑1 (MQ=12) aGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTa < 1:398636/70‑1 (MQ=12) ggCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTa < 1:2526099/71‑1 (MQ=11) ggCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCtt < 1:3840587/70‑1 (MQ=14) ggCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgat < 1:575863/52‑1 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct < 1:733193/48‑1 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctga > 1:1327297/1‑43 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCt < 1:1453856/68‑1 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTAt < 1:3147032/71‑1 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTAt < 1:719785/71‑1 (MQ=255) ggATAAGACGTTTACGCCGCATCCGGCATTTGTgc < 1:2301882/35‑1 (MQ=255) ggATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCAt > 1:3875956/1‑71 (MQ=255) tAAGACGTTTACGCCGCATCCGGCATTTGTGCGctga > 1:2564245/1‑37 (MQ=255) aGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctg < 1:3147794/41‑1 (MQ=255) cGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACa > 1:2312175/1‑70 (MQ=255) cGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACa > 1:2348861/1‑70 (MQ=255) tttATGCCGCATCCGGCAGTAATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTAt < 1:3129315/58‑1 (MQ=11) tttACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACaaa < 1:1937058/70‑1 (MQ=255) tttACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAAt < 1:1172589/71‑1 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc < 1:680451/70‑1 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc < 1:110868/70‑1 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc > 1:3586635/1‑70 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCt > 1:3760552/1‑71 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCt > 1:3697971/1‑71 (MQ=255) tACGCCGCATCCGGCAGTTGTACGcaggtgcctga > 1:2082527/1‑35 (MQ=9) tACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAttc > 1:1879168/1‑69 (MQ=11) cgccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTg < 1:20578/70‑1 (MQ=255) ccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTa < 1:1513807/70‑1 (MQ=255) gcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgc > 1:249184/1‑71 (MQ=255) cATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgc > 1:912032/1‑70 (MQ=255) cATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCt < 1:505567/37‑1 (MQ=11) aTCCGGCAGTCGTGTGCTGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:37824/48‑1 (MQ=14) cGGCATTTGTGCGCTGATGCCTGATGCGACGCTGa > 1:1050908/1‑35 (MQ=255) cGGCATTTGTGCGCTGATGCCTGATGCGACGCTGCCGCGTCTTATCATGCCTACAAATCTGTACGCGAAcc > 1:2331005/1‑71 (MQ=255) cGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAAcc > 1:3142606/1‑71 (MQ=255) ggCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg > 1:2075294/1‑71 (MQ=255) ggCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg < 1:2405781/71‑1 (MQ=255) ggCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg < 1:832762/71‑1 (MQ=255) ggCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg < 1:917430/71‑1 (MQ=255) tttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc < 1:418304/54‑1 (MQ=255) tttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTg < 1:2126657/56‑1 (MQ=255) tttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCATACAAATCTGTACGCGAACCGTAgg > 1:1900840/1‑71 (MQ=255) | TTGATTGAAAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGG > minE/222919‑223058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |