Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,502,847 | A→T | 30.3% | intergenic (‑220/‑700) | lrhA ← / → yfbQ | DNA‑binding transcriptional repressor/predicted aminotransferase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,502,847 | 0 | A | T | 30.3% | 114.3 / 64.4 | 79 | intergenic (‑220/‑700) | lrhA/yfbQ | DNA‑binding transcriptional repressor/predicted aminotransferase |
Reads supporting (aligned to +/- strand): ref base A (33/22); new base T (7/17); total (40/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 |
ACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTC > minE/1502779‑1502916 | acaTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGa > 1:289745/1‑69 (MQ=255) acaTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGa < 1:3553573/69‑1 (MQ=255) acaTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGa < 1:1207438/69‑1 (MQ=255) acaTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTa > 1:232643/1‑71 (MQ=255) acaTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATa > 1:3928135/1‑71 (MQ=255) caTTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATac < 1:80754/71‑1 (MQ=255) tGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATactca > 1:1753845/1‑71 (MQ=255) gTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATactca > 1:470890/1‑70 (MQ=255) ttCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTactcac < 1:2195507/70‑1 (MQ=255) ttCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTactcac < 1:2190586/70‑1 (MQ=255) cGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCtt < 1:667546/71‑1 (MQ=255) tataATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTc > 1:327660/1‑71 (MQ=255) taATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTa < 1:3678179/71‑1 (MQ=255) aaTGCCTGAAGTAGATCACATAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTa > 1:972086/1‑70 (MQ=255) aTGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTAtt > 1:4006738/1‑71 (MQ=255) tGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTAttt < 1:1479797/71‑1 (MQ=255) ccTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGa < 1:2801676/50‑1 (MQ=255) ccTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGa < 1:357314/50‑1 (MQ=255) ccTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTAt < 1:1891547/71‑1 (MQ=255) ccTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTAt < 1:2256550/71‑1 (MQ=255) cTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATg < 1:393690/71‑1 (MQ=255) gTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATg > 1:1120549/1‑71 (MQ=255) tAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATgc < 1:1177764/71‑1 (MQ=255) aGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATa > 1:744015/1‑44 (MQ=255) tCACAGAATATATCTTCAGGGATCGCATATCTATTAAGa > 1:3180621/1‑39 (MQ=255) cacaGAATATATCTTCAGGGATCGCATATCTATTAAGa > 1:1558923/1‑38 (MQ=255) cacaGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATg < 1:1661775/60‑1 (MQ=255) cacaGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGa > 1:3954124/1‑61 (MQ=255) cacaGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACa > 1:3737155/1‑63 (MQ=255) cacaGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCgtg > 1:518056/1‑71 (MQ=255) cacaGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCgtg > 1:442687/1‑71 (MQ=255) cacaGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCgtg > 1:2624598/1‑71 (MQ=255) acaGAATATATCTTCAGGGATCGCATATCTATTAAGATactcac > 1:3735528/1‑44 (MQ=255) acaGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATgcgc > 1:2089670/1‑67 (MQ=255) acaGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGTGCgtgt < 1:534625/71‑1 (MQ=255) acaGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCgtgt < 1:1033378/71‑1 (MQ=255) caGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGtt < 1:2028625/71‑1 (MQ=255) aGAATATATCTTCAGGGATCGCATATCTATTAAGa < 1:1048539/35‑1 (MQ=255) gAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTg > 1:3687517/1‑71 (MQ=255) gAATATATCTTCAGGGATCGCATATCTATTAAGATa > 1:418793/1‑36 (MQ=255) aaTATATCTTCAGGGATCGCATATCTATTAAGATactca > 1:2637748/1‑39 (MQ=255) aaTATATCTTCAGGGATCGCATATCTATTAAGATa > 1:3779668/1‑35 (MQ=255) aaTATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGAc > 1:2334241/1‑57 (MQ=255) aaTATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTg > 1:3976279/1‑70 (MQ=255) tatatCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTg < 1:3832181/68‑1 (MQ=255) tatatCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGa < 1:2803059/54‑1 (MQ=255) tatatCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGta > 1:1913642/1‑70 (MQ=255) tatCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGtata < 1:103527/70‑1 (MQ=255) tatCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGtata < 1:1084488/70‑1 (MQ=255) atCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaa > 1:2010214/1‑71 (MQ=255) cTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGttt > 1:2029763/1‑62 (MQ=255) ttCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGa > 1:298842/1‑48 (MQ=255) tCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgt > 1:2466573/1‑70 (MQ=255) cAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgta < 1:3672308/70‑1 (MQ=255) gATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATaaatgtaaatgt < 1:1865258/71‑1 (MQ=255) gATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATaaatgtaaatgt < 1:435687/71‑1 (MQ=255) gATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATaaatgtaaatgt > 1:1182717/1‑71 (MQ=255) aTCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGa < 1:1260385/41‑1 (MQ=255) aTCGCATATCTATTAAGATACTCACTCTTTTCTAtt < 1:2597608/36‑1 (MQ=255) tCGCATATCTATTAAGTTACTCACTCTTTTCTATTTAt < 1:3726942/38‑1 (MQ=255) tCGCATATCTATTAAGTTACTCACTCTTTTCTATTTAt < 1:942233/38‑1 (MQ=255) tCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGa < 1:3441999/71‑1 (MQ=255) cGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGttt > 1:3783208/1‑52 (MQ=255) gCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGt > 1:1534383/1‑71 (MQ=255) atatCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTcc > 1:380226/1‑71 (MQ=255) tatCTATTAAGTTACTCACTCTTTTCTATTTATGa < 1:3556977/35‑1 (MQ=255) tatCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCt < 1:2217414/71‑1 (MQ=255) atCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATaaatgtaaatgt < 1:443005/63‑1 (MQ=255) atCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGa > 1:305259/1‑65 (MQ=255) tCTATTAAGTTACTCACTCTTTTCTATTTATGAGTTGCGCGTGTTTGTAtaaatgtaaat < 1:1487086/60‑1 (MQ=255) tCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCtt < 1:2324556/70‑1 (MQ=255) tCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCt < 1:2546470/69‑1 (MQ=255) cTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTg > 1:1910663/1‑70 (MQ=255) tATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaat > 1:2448598/1‑58 (MQ=255) ttAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTg < 1:3628725/67‑1 (MQ=255) ttAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTc < 1:3350805/70‑1 (MQ=255) ttAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGtat < 1:2595586/46‑1 (MQ=255) ttAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGta > 1:2505197/1‑45 (MQ=255) ttAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaa > 1:2635033/1‑49 (MQ=255) ttAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAg < 1:2558355/61‑1 (MQ=255) ttAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTc < 1:3049178/70‑1 (MQ=255) aaGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaa > 1:2970932/1‑47 (MQ=255) aaGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTc > 1:2758474/1‑68 (MQ=255) aaGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCAc > 1:3388546/1‑71 (MQ=255) aaGATACTCACTCTTTTCTATTTATGACATGCCCGTGTTTCTAtaa > 1:2449939/1‑46 (MQ=255) aGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCAc < 1:3816204/70‑1 (MQ=255) gATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCActc < 1:3955546/71‑1 (MQ=255) | ACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGATACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCTTGTTCCACTC > minE/1502779‑1502916 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |