| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,530,607 | G→A | 12.5% | T69I (ACT→ATT) | yfcA ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,530,607 | 0 | G | A | 12.5% | 108.9 / 6.8 | 40 | T69I (ACT→ATT) | yfcA | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (15/20); new base A (2/3); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 | |||||||||||
GACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCC > minE/1530537‑1530675 | gACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGAtag < 1:2839174/71‑1 (MQ=255) aaaGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGAtagtag > 1:2740889/1‑71 (MQ=255) aTGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGaaa < 1:4073022/70‑1 (MQ=255) gCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATag < 1:1977031/70‑1 (MQ=255) gCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATag < 1:2877735/70‑1 (MQ=255) gATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAgc > 1:766009/1‑71 (MQ=255) atTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAgccgc > 1:2351308/1‑71 (MQ=255) ttGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAgccgcc > 1:4115542/1‑71 (MQ=255) tGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGccgccg > 1:994693/1‑71 (MQ=255) aGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCa < 1:1402535/71‑1 (MQ=255) tttCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGa < 1:3427675/54‑1 (MQ=255) tttCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAgg < 1:1273653/71‑1 (MQ=255) tCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGccgccg > 1:3757661/1‑65 (MQ=255) tCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGc < 1:2580823/70‑1 (MQ=255) tCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCt > 1:1199481/1‑71 (MQ=255) cTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCt < 1:361589/70‑1 (MQ=255) aTCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTGCa < 1:3814819/71‑1 (MQ=255) cGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGt > 1:340924/1‑71 (MQ=255) cTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGttt < 1:1183610/71‑1 (MQ=255) aaaCTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTa < 1:2866013/69‑1 (MQ=255) aaaCTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTAt > 1:2236372/1‑70 (MQ=255) aaCTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTg > 1:1910739/1‑71 (MQ=255) cTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGa < 1:544958/38‑1 (MQ=255) aCCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGc < 1:927106/71‑1 (MQ=255) aCCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGc < 1:2112482/71‑1 (MQ=255) aCCACTTTGCGGCGAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGc > 1:736483/1‑71 (MQ=255) tGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCa > 1:43788/1‑71 (MQ=255) cggcgAATAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCAtt < 1:3494863/71‑1 (MQ=255) gccgAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCa < 1:2075494/66‑1 (MQ=255) cgAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGc > 1:4063625/1‑71 (MQ=255) gAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTAt < 1:2935391/51‑1 (MQ=255) gAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTAt < 1:886421/51‑1 (MQ=255) gAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTAt < 1:679287/51‑1 (MQ=255) aaTAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCgg > 1:943549/1‑71 (MQ=255) aTAAAGTAGATAATAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCgg < 1:1888395/70‑1 (MQ=255) tAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCt < 1:2909729/38‑1 (MQ=255) tAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCgg > 1:2439780/1‑69 (MQ=255) tAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGga < 1:142346/71‑1 (MQ=255) aaGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGc > 1:3354542/1‑65 (MQ=255) aGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGgaga > 1:3944371/1‑70 (MQ=255) aGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGgaga > 1:3561607/1‑70 (MQ=255) gTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACa < 1:1985450/71‑1 (MQ=255) tagtagCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATcccc > 1:617145/1‑71 (MQ=255) agtagCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGc < 1:2695527/45‑1 (MQ=255) | GACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCC > minE/1530537‑1530675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |