| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,534,296 | G→A | 10.4% | A147T (GCA→ACA) | yfcN → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,534,296 | 0 | G | A | 10.4% | 195.7 / 11.4 | 67 | A147T (GCA→ACA) | yfcN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (24/36); new base A (3/4); total (27/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
TTTGCGCCTGCGTGATGCATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTG > minE/1534231‑1534364 | tttGCGCCTGCGTGATGCATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGACacaa > 1:1193087/1‑71 (MQ=255) ttGCGCCTGCGTGATGCATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCacaa > 1:3323498/1‑70 (MQ=255) tGCGCCTGCGTGATGCATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCacaac < 1:1196773/70‑1 (MQ=255) gcgcCTGCGTGATGCATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCacaaca < 1:1221960/70‑1 (MQ=255) gcgcCTGCGTGATGCATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCacaaca < 1:2493083/70‑1 (MQ=255) gCGTGATGCATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCAt > 1:73622/1‑71 (MQ=255) tGATGCATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCAtgt < 1:1698570/70‑1 (MQ=255) gCATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGtggctggc < 1:1386806/51‑1 (MQ=255) gCATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATgg > 1:1744499/1‑71 (MQ=255) cATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGc < 1:3500981/61‑1 (MQ=255) cATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGc < 1:2331876/61‑1 (MQ=255) cATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATgg < 1:2781242/70‑1 (MQ=255) aTGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGa > 1:799037/1‑66 (MQ=255) tGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATgg < 1:3026759/68‑1 (MQ=255) gggCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCtt > 1:1447977/1‑71 (MQ=255) ggCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATcc < 1:2463289/55‑1 (MQ=255) ggCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATc > 1:2011672/1‑54 (MQ=255) ggCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGa > 1:3129456/1‑63 (MQ=255) ggCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCttt > 1:3607884/1‑71 (MQ=255) ggCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCttt > 1:133178/1‑71 (MQ=255) ggCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCttt > 1:1813580/1‑71 (MQ=255) ggCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCA‑AACATCCGCATGTGATGGCCttt > 1:2580322/1‑70 (MQ=255) ggCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGACACAACATCCGCATGTGATGGCCttt > 1:3450016/1‑71 (MQ=255) gCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCg < 1:2285177/55‑1 (MQ=255) gCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCttt > 1:3172975/1‑70 (MQ=255) cACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTtca < 1:3171487/71‑1 (MQ=255) aGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCTCATGTGATGGCCTTTCATCAg < 1:2577082/68‑1 (MQ=255) aGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAg < 1:3649562/68‑1 (MQ=255) gCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCAtgt < 1:721647/51‑1 (MQ=255) cATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCt < 1:248920/58‑1 (MQ=255) tatTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGc < 1:1741204/44‑1 (MQ=255) atTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCaa < 1:2545427/70‑1 (MQ=255) ttttGAAGCAACAAACACCGCTGTGGCTGGCACAACAt < 1:1970247/38‑1 (MQ=255) ttttGAAGCAACAAACACCGCTGTGGCTGGCACAACAt < 1:973662/38‑1 (MQ=255) ttttGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGa > 1:932970/1‑48 (MQ=255) ttttGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGa > 1:973468/1‑48 (MQ=255) ttttGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCtt < 1:2373616/55‑1 (MQ=255) ttttGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCaa > 1:3342539/1‑69 (MQ=255) ttttGAAGCAACAAACACCGCTGTGGCTGGCACAACATACGCATGTGATGGCCTTTCATCAGGCACCaa > 1:656150/1‑69 (MQ=255) ttttGAAGCAACAAACACCGCTGTGGCTGACACAACATCCGCATGTGAt > 1:641767/1‑49 (MQ=255) ttGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCaaaa > 1:4143992/1‑69 (MQ=255) gAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAagag < 1:3055737/70‑1 (MQ=255) gAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAagag < 1:515477/70‑1 (MQ=255) gAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAagag > 1:1611070/1‑70 (MQ=255) gAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAagag < 1:240111/70‑1 (MQ=255) gCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATg < 1:1838431/71‑1 (MQ=255) caacaaACACCGCTGTGGCTGGCACAACATCCGGATGTGATGGCTTTtcat < 1:532607/51‑1 (MQ=255) aacaaacaCCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCtt < 1:1653070/46‑1 (MQ=255) acaaacaCCGCTGTGGCTGACACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCg < 1:2396392/71‑1 (MQ=255) caaacaCCGCTGTGGCTGGCACAACATCCGCATGTGATgg < 1:2459454/40‑1 (MQ=255) caaacaCCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTtca > 1:1533525/1‑47 (MQ=255) aaacaCCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAgg < 1:3939236/51‑1 (MQ=255) aaacaCCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGt < 1:1366032/71‑1 (MQ=255) aaacaCCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGt < 1:1734249/71‑1 (MQ=255) aaacaCCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGt < 1:1137641/71‑1 (MQ=255) aaacaCCGCTGTGGCTGACACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGt < 1:3655426/71‑1 (MQ=255) aacaCCGCTGTGGCTGGCACAACATCCGCATGTGa > 1:3162234/1‑35 (MQ=255) aacaCCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGt < 1:2550365/70‑1 (MQ=255) aacaCCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGt > 1:3359799/1‑70 (MQ=255) aacaCCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGt < 1:2555241/70‑1 (MQ=255) aacaCCGCTGTGGCGGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGt < 1:2656430/70‑1 (MQ=255) cacCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGt > 1:130118/1‑60 (MQ=255) cacCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTAt < 1:3548498/62‑1 (MQ=255) cacCGCTGTGGCTGACACAACATCCGCATGTGATGGCCTTtca < 1:2438490/43‑1 (MQ=255) ccGCTGTGGCTGGCACAACATCCGCATGTGAT‑GCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATgcg < 1:1917853/71‑1 (MQ=255) gCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATgcg > 1:2972632/1‑70 (MQ=255) tgtgGCTGACACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTAt < 1:2581895/56‑1 (MQ=255) tgtgCCTGGCACAACATCCCCATGTGATGGCCTTTCATCAGGc > 1:2557681/1‑43 (MQ=255) tggctggcACAACATCCGCATGTGATGGCCTTTCATCAGGCACCaaaa < 1:2577951/48‑1 (MQ=255) tggctggcACAACATCCGCATGTGATGGCCTTTCATCAGGCACCaaaa < 1:2048178/48‑1 (MQ=255) tggctggcACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCgtt > 1:1691801/1‑71 (MQ=255) ggctggcACAACATCCGCATGTGATGGCCTTTCATCAGGCACCaa < 1:101428/45‑1 (MQ=255) gctggcACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTa > 1:219338/1‑51 (MQ=255) ctggcACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCgttgtt < 1:3553834/71‑1 (MQ=255) ctggcACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCgttgtt < 1:3225999/71‑1 (MQ=255) ctggcACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCgttgtt < 1:255174/71‑1 (MQ=255) gcACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTa > 1:98955/1‑47 (MQ=255) gcACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCGttgttg < 1:328163/69‑1 (MQ=255) | TTTGCGCCTGCGTGATGCATGGGCACGGGAAGCATATTTTGAAGCAACAAACACCGCTGTGGCTGGCACAACATCCGCATGTGATGGCCTTTCATCAGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTG > minE/1534231‑1534364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |