| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,564,912 | G→A | 22.9% | intergenic (‑124/+168) | yfdE ← / ← glk | predicted CoA‑transferase, NAD(P)‑binding/glucokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,564,912 | 0 | G | A | 22.9% | 168.4 / 46.3 | 83 | intergenic (‑124/+168) | yfdE/glk | predicted CoA‑transferase, NAD(P)‑binding/glucokinase |
| Reads supporting (aligned to +/- strand): ref base G (31/33); new base A (9/10); total (40/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 | |||||||||||
AAATCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACG > minE/1564843‑1564982 | aaaTCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAg > 1:3267411/1‑70 (MQ=255)aaaTCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAg < 1:785322/70‑1 (MQ=255) cAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACa > 1:3189772/1‑70 (MQ=255) cATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGcc < 1:3083108/71‑1 (MQ=255) aTTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGa > 1:2140398/1‑57 (MQ=255) aTTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACa < 1:80212/60‑1 (MQ=255) aTTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTa > 1:210882/1‑71 (MQ=255) ttAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTa > 1:2194120/1‑70 (MQ=255) ttAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTAc < 1:3718953/71‑1 (MQ=255) ttAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTAc < 1:3294472/71‑1 (MQ=255) tAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTAc > 1:1799792/1‑70 (MQ=255) atcGTTAATTCCTCGTTGAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTAc > 1:3480495/3‑69 (MQ=255) atcGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTAc > 1:1384860/3‑69 (MQ=255) aaCGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTa > 1:2785382/1‑68 (MQ=255) cGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGt > 1:1741778/1‑64 (MQ=255) gTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGa > 1:2128414/1‑70 (MQ=255) ttAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCg < 1:3275820/58‑1 (MQ=255) tAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAAt > 1:923120/1‑55 (MQ=255) tAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCg > 1:2621014/1‑57 (MQ=255) tAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGAc > 1:3735762/1‑69 (MQ=255) tAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACa > 1:2388169/1‑70 (MQ=255) aTTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGa > 1:739047/1‑47 (MQ=255) aTTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACaaa > 1:244856/1‑70 (MQ=255) aTTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACaaa > 1:1020651/1‑70 (MQ=255) ccTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACa < 1:2502957/65‑1 (MQ=255) ccTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaataat > 1:3528328/1‑71 (MQ=255) ccTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaataat > 1:1563598/1‑71 (MQ=255) ccTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaataat > 1:3975205/1‑71 (MQ=255) ccTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaata > 1:854223/1‑69 (MQ=255) gTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAAt > 1:1632440/1‑46 (MQ=255) gTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAAt > 1:4140118/1‑46 (MQ=255) gTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCa < 1:1234348/70‑1 (MQ=255) gTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACaaa < 1:2041071/63‑1 (MQ=255) gTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGc < 1:3220759/69‑1 (MQ=255) ttCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCAtt < 1:2815245/71‑1 (MQ=255) ttCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCAtt < 1:274194/71‑1 (MQ=255) ttCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCAtt < 1:1079005/71‑1 (MQ=255) cAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACaaa > 1:1643828/1‑42 (MQ=255) cAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCa > 1:2717678/1‑67 (MQ=255) aGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTAc < 1:1832566/71‑1 (MQ=255) aGTAATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTAc < 1:959635/71‑1 (MQ=255) gTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataat < 1:266261/62‑1 (MQ=255) gTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaataat < 1:2799445/62‑1 (MQ=255) gTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACt > 1:1340844/1‑71 (MQ=255) gTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACt > 1:468703/1‑71 (MQ=255) tGATAGTTGACGAGTGAAATTATGAACGTGCAAAACaa < 1:821852/38‑1 (MQ=38) tGATAGTTGACGAGTGAAATTATGAACGTGCAAAACa < 1:190116/37‑1 (MQ=38) tGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACaaa < 1:2818191/57‑1 (MQ=255) gATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAAt > 1:440689/1‑39 (MQ=255) tAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGgag < 1:148603/71‑1 (MQ=255) gTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACaaa > 1:865397/1‑52 (MQ=255) ttgacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAaat < 1:1262057/50‑1 (MQ=255) ttgacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAt < 1:2907012/69‑1 (MQ=255) tCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAt > 1:64638/1‑71 (MQ=255) cgacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAt > 1:846183/1‑70 (MQ=255) gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGt > 1:2116572/1‑38 (MQ=255) gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCa < 1:2169567/43‑1 (MQ=255) gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaataat < 1:466551/53‑1 (MQ=255) gacgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAtt > 1:1576893/1‑70 (MQ=255) gacgaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACaaa > 1:2817539/1‑49 (MQ=255) acgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTaa < 1:2276222/71‑1 (MQ=255) cgaGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAAt < 1:3348332/71‑1 (MQ=255) gaGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTaa < 1:2627983/69‑1 (MQ=255) tGAAATTATGAGCGTGCAGAACAAATCGCCGTTACCAGACAaat < 1:4022230/44‑1 (MQ=255) tGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAaat < 1:1446806/44‑1 (MQ=255) tGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATg < 1:1292230/48‑1 (MQ=255) aaaTTATGAACGTGCAGCACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt > 1:937826/1‑71 (MQ=255) aaaTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGc > 1:1099615/1‑47 (MQ=255) aaaTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCt > 1:2389662/1‑71 (MQ=255) aaTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTg < 1:2296014/71‑1 (MQ=255) aaTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTg < 1:1902601/71‑1 (MQ=255) aaTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTg < 1:609582/71‑1 (MQ=255) aaTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTg < 1:1156322/71‑1 (MQ=255) aaTTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTg < 1:1966755/71‑1 (MQ=255) aTTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGt < 1:4065651/71‑1 (MQ=255) ttATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGaa < 1:4059868/57‑1 (MQ=255) ttATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGaa < 1:1111483/57‑1 (MQ=255) ttATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGaa < 1:361840/57‑1 (MQ=255) tATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCa > 1:241467/1‑44 (MQ=255) aTGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACg > 1:2032016/1‑71 (MQ=255) cGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAgt < 1:2806066/71‑1 (MQ=255) gTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAgtg < 1:2681702/71‑1 (MQ=255) tGCAGAACAAATCGCCGTTAGCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAgtgt < 1:3776513/71‑1 (MQ=255) tGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAgtgt < 1:1910929/71‑1 (MQ=255) aGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGAc > 1:3525406/1‑71 (MQ=255) gAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACg < 1:1204045/71‑1 (MQ=255) gAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACg > 1:2146060/1‑71 (MQ=255) gAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACg < 1:398870/71‑1 (MQ=255) | AAATCAATAACATGATTAACGTTAATTCCTCGTTCAGTGATAGTCGACGAGTGAAATTATGAACGTGCAGAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACG > minE/1564843‑1564982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |