| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,568,107 | C→T | 19.6% | G407R (GGG→AGG) | mntH ← | manganese/divalent cation transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,568,107 | 0 | C | T | 19.6% | 123.0 / 21.3 | 56 | G407R (GGG→AGG) | mntH | manganese/divalent cation transporter |
| Reads supporting (aligned to +/- strand): ref base C (23/22); new base T (5/6); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTT > minE/1568039‑1568176 | gCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCAAGCGCCGTCCcca > 1:3384708/1‑71 (MQ=255)gCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCAAGCGCCGTCCcca > 1:3379807/1‑71 (MQ=255) tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACcaa > 1:712594/1‑70 (MQ=255) ccgcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaa > 1:518673/1‑66 (MQ=255) cgcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCa < 1:2266581/71‑1 (MQ=255) tCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGata > 1:978527/1‑71 (MQ=255) tCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGata < 1:3396016/71‑1 (MQ=255) cGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATAtt > 1:1931087/1‑71 (MQ=255) cGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATAtt > 1:3152413/1‑71 (MQ=255) cGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATAtt > 1:559181/1‑71 (MQ=255) cGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATAtt > 1:3823472/1‑71 (MQ=255) ccAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTc < 1:818908/70‑1 (MQ=255) cAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGa > 1:2882974/1‑64 (MQ=255) aGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTc > 1:89894/1‑65 (MQ=255) gTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGcc < 1:4007457/70‑1 (MQ=255) gTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCAACCAACAACCAGATATTc > 1:1933937/1‑64 (MQ=255) ccAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACg < 1:3173580/70‑1 (MQ=255) gATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaac < 1:1562422/46‑1 (MQ=255) gATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGata > 1:2004107/1‑55 (MQ=255) gATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCa < 1:2765413/71‑1 (MQ=255) aTGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATAtt < 1:135493/56‑1 (MQ=255) aTGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAg < 1:3269817/71‑1 (MQ=255) tGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGa < 1:3762436/51‑1 (MQ=255) tGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCa < 1:1959068/49‑1 (MQ=255) tGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGc < 1:4066445/71‑1 (MQ=255) tGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGc < 1:101336/71‑1 (MQ=255) gCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGata > 1:2517391/1‑52 (MQ=255) gCGACGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGata > 1:3598780/1‑52 (MQ=255) gCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCa > 1:229394/1‑48 (MQ=255) gCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGca > 1:407086/1‑71 (MQ=255) aCGCTCATTCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACg < 1:148860/61‑1 (MQ=255) tCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcgcca > 1:2516690/1‑67 (MQ=255) tCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAAt > 1:750857/1‑71 (MQ=255) ttCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCAc < 1:2138776/71‑1 (MQ=255) ttCAACTACAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCAc < 1:3891811/71‑1 (MQ=255) ttCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATc < 1:2334176/69‑1 (MQ=255) ttCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCAc < 1:2858596/71‑1 (MQ=255) ttCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCCCCACGACCAGCACcaca < 1:123869/66‑1 (MQ=255) cAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGcccc > 1:1699661/1‑49 (MQ=255) cAACTACAATCCCAGCGCCGTCCCCACCAACAACAAGATATTCa > 1:923421/1‑44 (MQ=255) aaCTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCAcc > 1:2725090/1‑69 (MQ=255) cTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACcaca < 1:396063/61‑1 (MQ=255) cTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc < 1:3467598/71‑1 (MQ=255) cTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc < 1:2593883/71‑1 (MQ=255) aCAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCa > 1:2360164/1‑51 (MQ=255) aCAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCct > 1:1989092/1‑71 (MQ=255) aCAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCct > 1:141683/1‑71 (MQ=255) cAATCCCAGCGCCGTCCTCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCAc > 1:3664521/1‑63 (MQ=255) aaTCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgt > 1:3449315/1‑71 (MQ=255) cccAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCAc > 1:3292954/1‑41 (MQ=255) ccAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt < 1:770085/71‑1 (MQ=255) aGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGttt < 1:574478/71‑1 (MQ=255) aGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGttt < 1:825005/71‑1 (MQ=255) aGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGttt < 1:3299601/71‑1 (MQ=255) gcgcCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcaccac < 1:1281996/49‑1 (MQ=255) ccGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAAt < 1:2826491/49‑1 (MQ=255) cGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTAcgc > 1:4077066/1‑71 (MQ=255) gTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGcc > 1:1737592/1‑56 (MQ=255) cccACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGttt > 1:2527841/1‑71 (MQ=255) cccACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGttt > 1:2522862/1‑71 (MQ=255) cccACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGtt > 1:1534141/1‑70 (MQ=255) | GCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTT > minE/1568039‑1568176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |