| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,582,992 | T→C | 21.5% | S81S (TCA→TCG) | ligA ← | DNA ligase, NAD(+)‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,582,992 | 0 | T | C | 21.5% | 145.9 / 37.2 | 73 | S81S (TCA→TCG) | ligA | DNA ligase, NAD(+)‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (31/26); new base C (6/9); total (38/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01 | |||||||||||
GTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTA > minE/1582923‑1583061 | gttTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGt < 1:3947463/70‑1 (MQ=255)gttTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGt > 1:2328043/1‑70 (MQ=255)gttTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGt > 1:1584383/1‑70 (MQ=255) tttCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGa > 1:3763441/1‑69 (MQ=255) tCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAg < 1:1811291/70‑1 (MQ=255) tCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAg > 1:1239712/1‑70 (MQ=255) aCGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAg < 1:2553175/66‑1 (MQ=255) aCGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAg < 1:2548242/66‑1 (MQ=255) aCGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAg < 1:1635074/66‑1 (MQ=255) aCGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGCGACAGCATTg < 1:1690992/71‑1 (MQ=255) cGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGCGACAGCATTgg < 1:3074583/71‑1 (MQ=255) cTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACt < 1:3072363/70‑1 (MQ=255) cTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGCGACAGCATTGGTACtt > 1:2355017/1‑71 (MQ=255) cTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGCGACAGCATTGGTACtt < 1:1750119/71‑1 (MQ=255) gCACACGTTTGTTGAAAGCAAGAGAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCa > 1:3960222/1‑60 (MQ=255) cacaCTTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTc < 1:2053133/69‑1 (MQ=255) cacaCGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCAt < 1:4031940/71‑1 (MQ=255) acacGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATg < 1:3017930/71‑1 (MQ=255) acacGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGCGACAGc < 1:2591651/57‑1 (MQ=255) acacGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGCGACAGCATTGGTACTTCa > 1:1887926/1‑69 (MQ=255) acacGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGCGACAGCATTGGTACTTCa > 1:1739555/1‑69 (MQ=255) acGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACt > 1:2727565/1‑64 (MQ=255) acGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACt > 1:622224/1‑64 (MQ=255) acGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACt > 1:1719114/1‑64 (MQ=255) acGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTc < 1:3694488/66‑1 (MQ=255) cGTTTGTTGAAAGCAAGTAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGc < 1:1050179/54‑1 (MQ=255) cGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGa > 1:149291/1‑50 (MQ=255) tttGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTa > 1:36574/1‑71 (MQ=255) tgttgAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCAt < 1:3194911/63‑1 (MQ=255) tgttgAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATc > 1:1412798/1‑71 (MQ=255) gAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTa > 1:569332/1‑53 (MQ=255) gAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCAt > 1:2548565/1‑59 (MQ=255) aaaGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGt < 1:3660470/39‑1 (MQ=255) aaaGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGt < 1:1999378/39‑1 (MQ=255) gCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGaa < 1:1987331/71‑1 (MQ=255) cAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGa > 1:1327764/1‑69 (MQ=255) cAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGCGACAg < 1:3641862/40‑1 (MQ=255) aaGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGa > 1:1184483/1‑36 (MQ=255) aGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACtt < 1:3218686/49‑1 (MQ=255) aaaGCTTTCTTCATCAAAAACGTTATCCAGTGACAg < 1:460765/36‑1 (MQ=255) aaaGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAgccg > 1:1724216/1‑71 (MQ=255) aaaGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAgccg > 1:3239544/1‑71 (MQ=255) aaGGTTTCTTCATCAAAAACGTTATCCAGTGACAGCa > 1:115653/1‑37 (MQ=255) aaGCTTTCTTCATCAAAAACGTTATCCAGTGACAGc > 1:1790300/1‑36 (MQ=255) aaGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCa > 1:2109459/1‑37 (MQ=255) aaGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCa > 1:2000678/1‑37 (MQ=255) aaGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCa > 1:3215833/1‑37 (MQ=255) aaGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCa > 1:1657716/1‑37 (MQ=255) aaGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCAt > 1:1084020/1‑38 (MQ=255) aaGCTTTCTTCATCAAAAACGTTATCCAGCGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAgccgc > 1:1429169/1‑71 (MQ=255) cTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAg > 1:3405330/1‑71 (MQ=255) tttCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAgccg < 1:1245037/66‑1 (MQ=255) tttCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAgccg < 1:480190/66‑1 (MQ=255) tttCTTCATCAAAAACGTTATCCAGCGACAGCATTGGTACTTCa < 1:1141785/44‑1 (MQ=255) tcttcATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAgcgg > 1:4029119/1‑71 (MQ=255) ttcATCAAAAACGTTATCCAGTGACAGCATTGGTAcatcat > 1:3507040/1‑41 (MQ=255) ttcATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCAt > 1:1735154/1‑41 (MQ=255) catcaAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGgcgc < 1:325278/70‑1 (MQ=255) catcaAAAACGTTATCCAGCGACAGCATTGGTACTTCATGGCGTATCTg > 1:3650329/1‑49 (MQ=255) catcaAAAACGTTATCCAGCGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGgcgc < 1:2230650/70‑1 (MQ=255) tcaAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGaaa < 1:3685227/54‑1 (MQ=255) aaaaaCGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAgc > 1:3715757/1‑54 (MQ=255) aaaaaCGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAgcgc > 1:2808137/1‑71 (MQ=255) aaaaCGTTATCCAGTGACAGCATTGGTACTTCATGGCGTAt > 1:2375087/1‑41 (MQ=255) aaaaCGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGcc < 1:2030721/71‑1 (MQ=255) aaaaCGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGACAGCGGCGCa < 1:3984617/66‑1 (MQ=255) aaCGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTa > 1:2943690/1‑71 (MQ=255) aaCGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTa < 1:190410/71‑1 (MQ=255) aaCGTTATCCAGCGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAgccgc < 1:1991947/54‑1 (MQ=255) cGTTATCCAGCGACAGCATTGGTACTTCATGGCGTa < 1:3584580/36‑1 (MQ=255) ttATCCAGGGACAGCATTGGTACTTCATGGCGTATc > 1:1722996/1‑36 (MQ=255) ttATCCAGCGACAGCATTGGTACTTCATGGCGTATc > 1:206794/1‑36 (MQ=255) aTCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAgc > 1:1587627/1‑60 (MQ=255) tCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGtt < 1:326530/70‑1 (MQ=255) tCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGtt < 1:1970032/70‑1 (MQ=255) tCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTg < 1:3766188/71‑1 (MQ=255) cAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGa < 1:4133476/70‑1 (MQ=255) gTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTg < 1:888052/67‑1 (MQ=255) tGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCa < 1:506267/52‑1 (MQ=255) tGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTa < 1:282763/70‑1 (MQ=255) | GTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCTACACGTTGAGTA > minE/1582923‑1583061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |