| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,614,480 | C→T | 11.5% | intergenic (‑105/+112) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,614,480 | 0 | C | T | 11.5% | 154.0 / 9.3 | 52 | intergenic (‑105/+112) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base C (21/25); new base T (4/2); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
GCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAA > minE/1614414‑1614548 | gcggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCAt > 1:2160004/1‑71 (MQ=14)gcggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCAt > 1:2548088/1‑71 (MQ=39) cgTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCgg > 1:1725374/1‑71 (MQ=14) gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:3225980/71‑1 (MQ=39) gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:1481487/71‑1 (MQ=39) gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:149226/71‑1 (MQ=39) tAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:3780611/70‑1 (MQ=39) tAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCa < 1:2721790/71‑1 (MQ=38) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCaa > 1:2087881/1‑70 (MQ=37) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:3785453/1‑71 (MQ=38) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:2293539/1‑71 (MQ=38) gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:1054854/71‑1 (MQ=255) gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:2316897/71‑1 (MQ=255) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc > 1:2086139/1‑71 (MQ=255) tATCCGCCCTACATGTGCAATCCCGTAGGCAGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcg > 1:1002052/1‑71 (MQ=255) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAATACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:15835/1‑71 (MQ=255) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAtt > 1:3390398/1‑64 (MQ=40) ccTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCAc < 1:441987/70‑1 (MQ=17) tACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCa < 1:935385/53‑1 (MQ=2) tACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:194964/52‑1 (MQ=35) aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCa > 1:3041866/1‑58 (MQ=255) cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg < 1:3246438/69‑1 (MQ=255) cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg < 1:1599352/69‑1 (MQ=255) cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCg > 1:3317216/1‑71 (MQ=255) cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCg < 1:509452/71‑1 (MQ=255) cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCg > 1:1241331/1‑71 (MQ=255) aTGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:3735406/1‑52 (MQ=255) aTGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:1215143/1‑52 (MQ=255) aTGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:1820989/1‑52 (MQ=255) gtgCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCa < 1:1707396/48‑1 (MQ=38) gtgCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCAc < 1:3527178/63‑1 (MQ=255) tgCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTc > 1:659091/1‑37 (MQ=40) aTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcggc > 1:746497/1‑71 (MQ=255) tCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcggcg > 1:2300886/1‑71 (MQ=255) cccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGa > 1:622255/1‑63 (MQ=255) ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTg < 1:1277451/71‑1 (MQ=255) ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTg < 1:3237139/71‑1 (MQ=255) ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTg < 1:1959502/71‑1 (MQ=255) gTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTgga > 1:1908908/1‑69 (MQ=11) gTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGaa > 1:107921/1‑71 (MQ=255) ggCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGc < 1:987206/71‑1 (MQ=255) ggCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGc > 1:1985257/1‑71 (MQ=14) gccggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGcc > 1:4140263/4‑71 (MQ=17) cTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCt < 1:1344270/71‑1 (MQ=255) tGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCg < 1:3679221/52‑1 (MQ=255) gATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCtt < 1:2625794/69‑1 (MQ=255) aTAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATc > 1:2793928/1‑71 (MQ=255) tAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTg < 1:3387212/59‑1 (MQ=255) aGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcgg < 1:3351702/53‑1 (MQ=255) gcgGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTa < 1:496916/71‑1 (MQ=255) gacAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTa < 1:312122/58‑1 (MQ=14) cGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGaa > 1:841146/1‑47 (MQ=40) gTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCaa < 1:1713102/71‑1 (MQ=255) | GCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAA > minE/1614414‑1614548 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |