| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,634,087 | C→T | 14.6% | L75L (CTC→CTT) | acrD → | aminoglycoside/multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,634,087 | 0 | C | T | 14.6% | 139.7 / 14.8 | 55 | L75L (CTC→CTT) | acrD | aminoglycoside/multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base C (23/23); new base T (3/5); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
GCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGCAGGCACC > minE/1634022‑1634156 | gCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGta < 1:2482017/71‑1 (MQ=255)gCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTAATGta < 1:528883/71‑1 (MQ=255) ccAGACGCTGGAAACCACCGTGACCCAGGTTATCGCGCAAAATATGACCGGCCTCGATAATCTCAGGtata > 1:204373/1‑71 (MQ=255) ccAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCa > 1:3954966/1‑65 (MQ=255) aCGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTc < 1:161985/71‑1 (MQ=255) cGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCa > 1:2274060/1‑71 (MQ=255) cGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCa > 1:2948803/1‑71 (MQ=255) cGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCa > 1:2503643/1‑71 (MQ=255) gAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCa < 1:3130294/71‑1 (MQ=255) aaaaCACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCag < 1:1546240/71‑1 (MQ=255) aaaCACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTatat > 1:2252343/1‑60 (MQ=255) aaaCACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTatat > 1:1285510/1‑60 (MQ=255) aaaCACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCa > 1:3311404/1‑69 (MQ=255) aaCACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCagag < 1:1181275/71‑1 (MQ=255) aaCACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCagag < 1:1458802/71‑1 (MQ=255) aaCACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCagag < 1:1761784/71‑1 (MQ=255) acacCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTatat > 1:2871488/1‑58 (MQ=255) ccAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCa > 1:1382243/1‑52 (MQ=255) aGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGt > 1:595305/1‑62 (MQ=255) aGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTgg > 1:2958526/1‑64 (MQ=255) aGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGt < 1:3699516/71‑1 (MQ=255) ggTTATCGAGCAAAATATGACCGGCCTCGATAATCTCGTGta < 1:225552/42‑1 (MQ=255) ggTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGta < 1:1984073/42‑1 (MQ=255) ggTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGt > 1:2900836/1‑70 (MQ=255) gattATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCa > 1:216393/3‑54 (MQ=255) gTTATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCa < 1:1607327/71‑1 (MQ=255) gTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCAtct < 1:3381182/51‑1 (MQ=255) gTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCAtct < 1:1030298/51‑1 (MQ=255) gTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCAtct < 1:2187855/51‑1 (MQ=255) ttATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAg < 1:1701069/71‑1 (MQ=255) tATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCAtc > 1:284302/1‑48 (MQ=255) tATCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAgg > 1:3715107/1‑71 (MQ=255) tCGAGCAAAATATGACCGGCCTCGATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCa < 1:772376/71‑1 (MQ=255) gCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCa > 1:2370473/1‑50 (MQ=255) aaaaTATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCg < 1:4075743/57‑1 (MQ=255) aaaaTATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGt < 1:3963719/70‑1 (MQ=255) aaaTATGACCGGCCTCGATAATCTCATGTATATGTCATCTCa > 1:486832/1‑42 (MQ=255) aaTATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCAt > 1:654204/1‑64 (MQ=255) aaTATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCAc > 1:1671088/1‑71 (MQ=255) tatGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCAc > 1:591143/1‑51 (MQ=255) tatGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCgg > 1:1610411/1‑54 (MQ=255) tatGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAgga > 1:2231945/1‑59 (MQ=255) tGACCGGCCTCGATAATCTCATGTATATGTCAtct < 1:4130363/35‑1 (MQ=255) tGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTa < 1:2564880/71‑1 (MQ=255) cGGCCTCGATAATCTCATGTATATGTCATCTCAGAGc < 1:3126418/37‑1 (MQ=255) gCCTCGATAATCTCATTTATATGTCATCTCAGAGc < 1:520423/35‑1 (MQ=255) gCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTa < 1:350604/65‑1 (MQ=255) cTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCa > 1:808989/1‑58 (MQ=255) cTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTaa < 1:203591/71‑1 (MQ=255) gATAATCTTATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGt < 1:1926354/63‑1 (MQ=255) gATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAgg < 1:38375/46‑1 (MQ=255) gATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAg < 1:3375090/70‑1 (MQ=255) aTAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGCa < 1:3688995/71‑1 (MQ=255) aaTCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAgg > 1:2314523/1‑43 (MQ=255) aTCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGCAgg > 1:395182/1‑70 (MQ=255) ctcATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGCAGGCAc > 1:134227/1‑71 (MQ=255) tcATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCAt > 1:2061047/1‑42 (MQ=255) tcATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTaa > 1:3290275/1‑54 (MQ=255) tcATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGCAGGCAcc > 1:2586869/1‑71 (MQ=255) cATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGtt > 1:1514885/1‑56 (MQ=255) | GCCCAGACGCTGGAAAACACCGTGACCCAGGTTATCGAGCAAAATATGACCGGCCTCGATAATCTCATGTATATGTCATCTCAGAGCAGTGGCACCGGTCAGGCATCTGTCACTTTAAGTTTTAAAGCAGGCACC > minE/1634022‑1634156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |