| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,657,320 | A→G | 9.8% | S531G (AGT→GGT) | ppk → | polyphosphate kinase, component of RNA degradosome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,657,320 | 0 | A | G | 9.8% | 140.1 / 7.0 | 51 | S531G (AGT→GGT) | ppk | polyphosphate kinase, component of RNA degradosome |
| Reads supporting (aligned to +/- strand): ref base A (23/23); new base G (2/3); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
CAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATG > minE/1657251‑1657380 | cAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAg > 1:2234805/1‑71 (MQ=255) aaaCTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTg > 1:3596358/1‑71 (MQ=255) aCTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg > 1:3442023/1‑70 (MQ=255) cTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTa > 1:3506018/1‑71 (MQ=255) cccGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGt > 1:2129278/1‑68 (MQ=255) ccgccgCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTAt < 1:710066/69‑1 (MQ=255) ccgccgCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCa > 1:3454768/1‑71 (MQ=255) ccgccTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCAccc > 1:2395619/1‑71 (MQ=255) ttGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTgg < 1:301281/56‑1 (MQ=255) ttGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGc > 1:1097811/1‑70 (MQ=255) gTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTTGTATCACCCTGAAGCTa < 1:602111/70‑1 (MQ=255) gTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTa > 1:656296/1‑70 (MQ=255) gTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTa > 1:2356101/1‑70 (MQ=255) gTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTa < 1:1629443/70‑1 (MQ=255) tATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCa > 1:2343903/1‑58 (MQ=255) gAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATaa > 1:3769316/1‑71 (MQ=255) aaaTGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTg < 1:3413757/48‑1 (MQ=255) aaaTGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATaa < 1:1423077/70‑1 (MQ=255) aaaTGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCAc > 1:2035896/1‑55 (MQ=255) aaaTGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCAc > 1:3120281/1‑55 (MQ=255) aaTGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGt < 1:689256/49‑1 (MQ=255) aaTGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAAc < 1:3653410/70‑1 (MQ=255) tggtggACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTaaa < 1:3580130/64‑1 (MQ=255) ggtggACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTg > 1:1522951/1‑71 (MQ=255) tggACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTc < 1:491835/71‑1 (MQ=255) gACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGa > 1:3791436/1‑71 (MQ=255) aCCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGAt < 1:1627236/71‑1 (MQ=255) ccGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATa < 1:3677735/71‑1 (MQ=255) ccGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATa < 1:3312993/71‑1 (MQ=255) ccGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATa < 1:345299/71‑1 (MQ=255) cgcgAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATa > 1:2371014/1‑70 (MQ=255) cgAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCg > 1:2780669/1‑65 (MQ=255) aTCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTg > 1:35183/1‑58 (MQ=255) tCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAAc > 1:3830258/1‑53 (MQ=255) gCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTgg < 1:3474683/70‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCGCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTg < 1:3991793/71‑1 (MQ=255) cAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTg < 1:2803551/71‑1 (MQ=255) cgcgCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGagc < 1:4125235/71‑3 (MQ=255) cgcgCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATc < 1:823551/71‑1 (MQ=255) cgcAGCAAGGGCTGCCCAGTGGTATCACGCTGAAGCTAAATAACCTTGTCGATaaa < 1:1747418/56‑1 (MQ=255) agcaAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAATGCCTGGTTGATCGTCTg < 1:2628586/71‑1 (MQ=255) agcaAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTg < 1:601146/71‑1 (MQ=255) agcaAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTg < 1:1798984/71‑1 (MQ=255) agcaAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTg < 1:1477810/71‑1 (MQ=255) gcaAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTg < 1:3084797/70‑1 (MQ=255) caAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTc < 1:3060099/45‑1 (MQ=255) aaGGGCTGCCCGGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTAt > 1:1818536/1‑71 (MQ=255) aaGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTAt > 1:3438900/1‑71 (MQ=255) aGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAg > 1:3904707/1‑50 (MQ=255) aGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAg > 1:1138095/1‑50 (MQ=255) aGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATg > 1:1039812/1‑71 (MQ=255) | CAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATG > minE/1657251‑1657380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |