| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,670,644 | A→G | 20.0% | I232T (ATT→ACT) | yfgL ← | protein assembly complex, lipoprotein component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,670,644 | 0 | A | G | 20.0% | 81.0 / 17.1 | 40 | I232T (ATT→ACT) | yfgL | protein assembly complex, lipoprotein component |
| Reads supporting (aligned to +/- strand): ref base A (12/20); new base G (3/5); total (15/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCC > minE/1670574‑1670710 | gTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGaaa < 1:2932110/71‑1 (MQ=255) gAGTCGTGTCAACATCGCTCAGACGGTCAATTTTGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCaa < 1:2708139/71‑1 (MQ=255) gAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCaa < 1:31849/71‑1 (MQ=255) tCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAatc < 1:582431/71‑1 (MQ=255) tgtCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACgct < 1:3140006/57‑1 (MQ=255) tCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTg > 1:23938/1‑71 (MQ=255) cATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGccc < 1:3629419/71‑1 (MQ=255) aTCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCtgctgc < 1:1910444/55‑1 (MQ=255) aTCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCt > 1:145307/1‑65 (MQ=255) tCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGctgctg < 1:2450292/53‑1 (MQ=255) gCTCAGACGGTCAATTTCGGTATAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGtt > 1:856422/1‑71 (MQ=255) gCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATAc > 1:2382113/1‑44 (MQ=255) gCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGtt > 1:2625904/1‑71 (MQ=255) cAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCa > 1:3561592/1‑71 (MQ=255) cAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTcc < 1:2698825/70‑1 (MQ=255) aGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCTAAATCATCTGGCCCTGTTCCa < 1:1665107/70‑1 (MQ=255) aGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTcc < 1:1826131/69‑1 (MQ=255) aCGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAatca > 1:1449130/1‑53 (MQ=255) gTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAatcat < 1:618855/51‑1 (MQ=255) tCAATTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCAc > 1:1373405/1‑71 (MQ=255) cAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACt > 1:200169/1‑71 (MQ=255) aaTTTCGGTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATc > 1:1598695/1‑64 (MQ=255) tttCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCaa < 1:567899/41‑1 (MQ=255) tttCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAg < 1:3708827/64‑1 (MQ=255) ttCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCaaa > 1:478751/1‑41 (MQ=255) ggTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGa < 1:1570755/71‑1 (MQ=255) gTAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAg < 1:327016/59‑1 (MQ=255) gTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAatc < 1:805068/39‑1 (MQ=255) tAGAACCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCa < 1:672792/54‑1 (MQ=255) tAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCt < 1:704833/67‑1 (MQ=255) aCCGGTCGCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGAc < 1:3078395/71‑1 (MQ=255) aCCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGAc < 1:2849015/71‑1 (MQ=255) ggTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCAt < 1:2813622/71‑1 (MQ=255) gTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGa > 1:3682756/1‑66 (MQ=255) gCCTGGGAAGTACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCt < 1:2599830/56‑1 (MQ=255) gCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATc > 1:1270500/1‑71 (MQ=255) ccTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCg < 1:1260442/71‑1 (MQ=255) tGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTgc < 1:1913076/50‑1 (MQ=255) gggAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAg < 1:3654045/43‑1 (MQ=255) ggAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATGAGCACTGCGCTGACGCGACCATTATCGcccc > 1:1580169/1‑71 (MQ=255) ggAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGcccc > 1:2396935/1‑71 (MQ=255) | GTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCC > minE/1670574‑1670710 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |