| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 222,997 | T→A | 22.8% | intergenic (+52/‑128) | lpcA → / → prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 222,997 | 0 | T | A | 22.8% | 116.0 / 30.2 | 53 | intergenic (+52/‑128) | lpcA/prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Reads supporting (aligned to +/- strand): ref base T (17/23); new base A (3/9); total (21/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01 | |||||||||||
AAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAAT > minE/222927‑223064 | aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:3471632/71‑1 (MQ=255)aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:2535133/71‑1 (MQ=255)aaaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:2110079/71‑1 (MQ=255) aaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctg < 1:298310/71‑1 (MQ=255) aaaGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct > 1:1579794/1‑70 (MQ=255) agagATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgat > 1:1190847/1‑71 (MQ=255) aTGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct > 1:3590817/1‑71 (MQ=255) aTGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct < 1:404501/71‑1 (MQ=255) ggTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatg > 1:421964/1‑66 (MQ=255) ggTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatg > 1:510023/1‑66 (MQ=255) ttAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatg > 1:2509624/1‑71 (MQ=255) ttAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatg > 1:1424715/1‑71 (MQ=255) ttAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgatg > 1:430694/1‑71 (MQ=255) agtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct < 1:1477924/64‑1 (MQ=255) agtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCg < 1:389116/70‑1 (MQ=255) agtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCg < 1:2909023/70‑1 (MQ=255) gtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatg < 1:2619229/60‑1 (MQ=255) gtaagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:3301149/56‑1 (MQ=255) gtaagtCTGGCCTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:1945690/56‑1 (MQ=255) aagtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct < 1:3887419/61‑1 (MQ=255) agtCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgc < 1:4090573/58‑1 (MQ=255) tGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcg > 1:3623050/1‑71 (MQ=255) tGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcg > 1:3618317/1‑71 (MQ=255) gCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGa > 1:1322626/1‑67 (MQ=255) gCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcgc < 1:413923/70‑1 (MQ=255) gCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAc < 1:3192193/68‑1 (MQ=255) tgTAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCt < 1:2882678/61‑1 (MQ=9) cGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGt > 1:238771/1‑71 (MQ=14) cGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:1250631/46‑1 (MQ=255) gTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGct < 1:2129690/45‑1 (MQ=255) gTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTc < 1:1687108/71‑1 (MQ=255) tAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTa < 1:2568575/71‑1 (MQ=12) tAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTa > 1:2702424/1‑71 (MQ=12) aGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTa < 1:398636/70‑1 (MQ=12) ggCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTa < 1:2526099/71‑1 (MQ=11) ggCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCtt < 1:3840587/70‑1 (MQ=14) ggCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctgat < 1:575863/52‑1 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcct < 1:733193/48‑1 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGctga > 1:1327297/1‑43 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCt < 1:1453856/68‑1 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTAt < 1:3147032/71‑1 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTAt < 1:719785/71‑1 (MQ=255) ggATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCAt > 1:3875956/1‑71 (MQ=255) tAAGACGTTTACGCCGCATCCGGCATTTGTGCGctga > 1:2564245/1‑37 (MQ=255) aGACGTTTACGCCGCATCCGGCATTTGTGCGctgatgcctg < 1:3147794/41‑1 (MQ=255) cGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACa > 1:2348861/1‑70 (MQ=255) cGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACa > 1:2312175/1‑70 (MQ=255) tttATGCCGCATCCGGCAGTAATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTAt < 1:3129315/58‑1 (MQ=11) tttACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACaaa < 1:1937058/70‑1 (MQ=255) tttACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAAt < 1:1172589/71‑1 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc < 1:680451/70‑1 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc < 1:110868/70‑1 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc > 1:3586635/1‑70 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCt > 1:3697971/1‑71 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCt > 1:3760552/1‑71 (MQ=255) tACGCCGCATCCGGCAGTTGTACGcaggtgcctga > 1:2082527/1‑35 (MQ=9) tACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAttc > 1:1879168/1‑69 (MQ=11) cgccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTg < 1:20578/70‑1 (MQ=255) ccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTa < 1:1513807/70‑1 (MQ=255) gcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgc > 1:249184/1‑71 (MQ=255) cATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgc > 1:912032/1‑70 (MQ=255) cATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCt < 1:505567/37‑1 (MQ=11) aTCCGGCAGTCGTGTGCTGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:37824/48‑1 (MQ=14) cGGCATTTGTGCGCTGATGCCTGATGCGACGCTGa > 1:1050908/1‑35 (MQ=255) cGGCATTTGTGCGCTGATGCCTGATGCGACGCTGCCGCGTCTTATCATGCCTACAAATCTGTACGCGAAcc > 1:2331005/1‑71 (MQ=255) cGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAAcc > 1:3142606/1‑71 (MQ=255) ggCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg < 1:917430/71‑1 (MQ=255) ggCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg < 1:832762/71‑1 (MQ=255) ggCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg > 1:2075294/1‑71 (MQ=255) ggCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg < 1:2405781/71‑1 (MQ=255) tttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc < 1:418304/54‑1 (MQ=255) tttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTg < 1:2126657/56‑1 (MQ=255) tttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCATACAAATCTGTACGCGAACCGTAgg > 1:1900840/1‑71 (MQ=255) ttGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACaa < 1:1963291/50‑1 (MQ=11) ttGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACaa < 1:2458922/50‑1 (MQ=11) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:2080797/1‑42 (MQ=14) gtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc < 1:3783617/70‑1 (MQ=255) gcgcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCg < 1:3882356/69‑1 (MQ=255) cgcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaat < 1:2928515/71‑1 (MQ=255) cgcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaa > 1:72883/1‑70 (MQ=255) tgatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGt < 1:1416463/48‑1 (MQ=255) | AAAAGAGATGGTTAAGTAAGTCTGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAAT > minE/222927‑223064 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |