| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,695,634 | G→A | 11.1% | Q126* (CAG→TAG) | yfhQ ← | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,695,634 | 0 | G | A | 11.1% | 153.8 / 8.9 | 54 | Q126* (CAG→TAG) | yfhQ | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (24/24); new base A (2/4); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
AACCGCCATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGC > minE/1695563‑1695697 | aaCCGCCATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAAT‑‑CGACATGATAATGGCATTTCTGc < 1:354437/71‑1 (MQ=255) cGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCtt > 1:2609688/1‑71 (MQ=255) cAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCAt > 1:910072/1‑71 (MQ=255) gTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCtt < 1:208151/65‑1 (MQ=255) gTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCtt < 1:2050098/65‑1 (MQ=255) gTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCtt < 1:3883248/65‑1 (MQ=255) tCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGt > 1:1400010/1‑70 (MQ=255) tCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGt > 1:3786611/1‑70 (MQ=255) gCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTc < 1:2017061/61‑1 (MQ=255) gCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCaa < 1:809149/70‑1 (MQ=255) cGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCtt < 1:1969227/59‑1 (MQ=255) cGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCtt < 1:845223/59‑1 (MQ=255) gAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGcc < 1:203985/71‑1 (MQ=255) gAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGcc < 1:2260840/71‑1 (MQ=255) gCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTACAACTCTTCATTGGTCAAGcc < 1:2245419/69‑1 (MQ=255) tGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAActc < 1:2157796/52‑1 (MQ=255) tGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCtt < 1:59455/54‑1 (MQ=255) tGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTACAACTCtt < 1:393293/54‑1 (MQ=255) aTTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACgcgc > 1:415618/1‑71 (MQ=255) ttCCGGGTTAGCCGCAATCGCGACATGATATTGGCATTTCTGCAACTCTTCATTGGTCAAGc > 1:1596530/1‑62 (MQ=255) ttCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAAc < 1:2854336/46‑1 (MQ=255) ttCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAAc < 1:2293134/46‑1 (MQ=255) ttCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCt > 1:2507616/1‑71 (MQ=255) ttCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCt > 1:522146/1‑71 (MQ=255) ccGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGAc > 1:186003/1‑64 (MQ=255) gggTTAGCCGCAATCGCGACATGATAATGGCATTTCTACAACTCTTCATTGGTCAAGCCGACGCGCTcgcg < 1:1483664/71‑1 (MQ=255) ttAGCCGCAATCGCGACATGATAATGGCATTTCTGCAAc < 1:1678977/39‑1 (MQ=255) ttAGCCGCAATCGCGACATGATAATGGCATTTCTACAACTCTTCATTGGTCAAGCCGACGCGCTCGCGAc > 1:3296318/1‑70 (MQ=255) tAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGAcc > 1:2952496/1‑70 (MQ=255) aGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGAcc > 1:479282/1‑69 (MQ=255) aGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:357242/70‑1 (MQ=255) aGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa > 1:3684619/1‑70 (MQ=255) aGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:3208332/70‑1 (MQ=255) gCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaa < 1:1168564/70‑1 (MQ=255) cGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTg > 1:173738/1‑43 (MQ=255) cGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTc > 1:3348117/1‑46 (MQ=255) gCAATCGCGACATGATAATGGCATTTCTACAACTCTTCATTGGTCAAGcc < 1:1154782/50‑1 (MQ=255) aaTCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:3545380/1‑66 (MQ=255) cgcgACAAGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGa > 1:3191931/1‑47 (MQ=255) gcgACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:607866/1‑62 (MQ=255) cgACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGcc < 1:1138253/71‑1 (MQ=255) gACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGa > 1:11292/1‑44 (MQ=255) tGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCACTcgc < 1:3091255/49‑1 (MQ=255) gATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCggg > 1:3590394/1‑71 (MQ=255) tGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTAt < 1:1511481/70‑1 (MQ=255) ggCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAgc > 1:2251990/1‑56 (MQ=255) ggCATTTCTACAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCa > 1:2741442/1‑60 (MQ=255) gCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGc < 1:3131534/71‑1 (MQ=255) cATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGc > 1:3679179/1‑71 (MQ=255) aTTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:1713659/44‑1 (MQ=255) aTTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAacac < 1:2592078/49‑1 (MQ=255) tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCaaa > 1:3264870/1‑43 (MQ=255) tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGc > 1:771226/1‑67 (MQ=255) tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGc > 1:2045653/1‑67 (MQ=255) | AACCGCCATCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGC > minE/1695563‑1695697 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |