| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,695,852 | G→A | 38.9% | A53V (GCC→GTC) | yfhQ ← | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,695,852 | 0 | G | A | 38.9% | 43.0 / 56.2 | 54 | A53V (GCC→GTC) | yfhQ | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (21/12); new base A (11/10); total (32/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGATTAACCAGCCACAGAT > minE/1695783‑1695915 | accaGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGc > 1:283306/1‑71 (MQ=255)accaGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGc > 1:390615/1‑71 (MQ=255)accaGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGc > 1:1915911/1‑71 (MQ=255) ccaGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGAcc < 1:2575458/71‑1 (MQ=255) caGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGAccc > 1:1026146/1‑71 (MQ=255) caGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGAccc > 1:2849674/1‑71 (MQ=255) ggCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCTATCACATCGCTGACCCct < 1:3757315/71‑1 (MQ=255) ggCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGcccc > 1:2501840/1‑70 (MQ=255) gCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCctg > 1:2576381/1‑71 (MQ=255) gCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCct < 1:701253/70‑1 (MQ=255) gCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGACCCct < 1:2711734/70‑1 (MQ=255) cAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCtgctgc > 1:2084675/1‑71 (MQ=255) aCCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGACCCctgct > 1:1610681/1‑67 (MQ=255) gCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGACCCctg < 1:3115453/61‑1 (MQ=255) cTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAg > 1:1989147/1‑61 (MQ=255) cTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGcc > 1:3977198/1‑71 (MQ=255) cTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGc > 1:2200837/1‑70 (MQ=255) ttCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCa < 1:3355094/59‑1 (MQ=255) cgtcgAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAg > 1:1586953/1‑58 (MQ=255) gtcgAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGACCCCtgctgc < 1:1764456/54‑1 (MQ=255) gAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGACCCCTGCTGCCAGGGCAATCGCCTGGGAGt > 1:1687568/1‑71 (MQ=255) aGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGACCCCTGCTGCCAGGGCAATCGCCTGGGAGTc < 1:2948568/71‑1 (MQ=255) gCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCg < 1:827858/71‑1 (MQ=255) gCGTATCGACGATGTGAGCATTACCAATCACATCGCTGACCCCTGCTGCCAGGGCAATCGCCTGGGAGTCg < 1:2181021/71‑1 (MQ=255) tATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAAt > 1:1552463/1‑55 (MQ=255) tCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGttt < 1:3804908/71‑1 (MQ=255) gacgaTGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCa > 1:1769848/1‑71 (MQ=255) cgaTGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCAcc < 1:3410765/71‑1 (MQ=255) cgaTGTGAGCATTACCAATCACATCGCTGACCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCAcc < 1:870772/71‑1 (MQ=255) gaTGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCa < 1:2478714/71‑1 (MQ=255) gaTGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCa < 1:3215728/71‑1 (MQ=255) tgtgAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGt > 1:216531/1‑71 (MQ=255) gtgAGCATTACCAATCACATCGCTGACCCCTGCTGCCAGGGCaa > 1:1637807/1‑44 (MQ=255) gtgAGCATTACCAATCACATCGCTGACCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTg > 1:3448824/1‑71 (MQ=255) gCATTACCAATCACATCGCTGACCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGAtt < 1:649398/71‑1 (MQ=255) cATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTc < 1:1916477/52‑1 (MQ=255) cATTACCAATCACATCGCTGACCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGAtt > 1:3553896/1‑70 (MQ=255) ttACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCAcc < 1:600597/60‑1 (MQ=255) ttACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGATTAAc < 1:2716131/71‑1 (MQ=255) ttACCAATCACATCGCTGACCCCTGCTGCCAGGGCaa < 1:2170921/37‑1 (MQ=255) aCCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAg > 1:2602539/1‑60 (MQ=255) aCCAATCACATCGCTGACCCCTGCTGCCAGGGCAAt > 1:3766156/1‑36 (MQ=255) aCCAATCACATCGCTGACCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGATTAAc > 1:607069/1‑69 (MQ=255) aCCAATCACATCGCTGACCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGATTAAc > 1:2248869/1‑69 (MQ=255) ccAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGATTAACCAg > 1:3703034/1‑71 (MQ=255) ccAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGATTAACCAg > 1:3995261/1‑71 (MQ=255) cAATCACATCGCTGACCCCTGCTGCCAGGGCAATCGc > 1:117312/1‑37 (MQ=255) tCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGATTAACCAGCca > 1:751132/1‑70 (MQ=255) cacaTCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCAc > 1:4137597/1‑51 (MQ=255) acaTCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCaa > 1:324953/1‑49 (MQ=255) acaTCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGATTAACCAGCca > 1:3747210/1‑68 (MQ=255) acaTCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGATTAACCAGCCACAg < 1:243946/71‑1 (MQ=255) caTCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGATTAACCAGCCACAGa < 1:2228465/71‑1 (MQ=255) aTCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGATTAACCAGCCACAGAt > 1:248360/1‑71 (MQ=255) | ACCAGGCTACAACCGGCTAACGCTTCGTCGAGCGTATCGACGATGTGAGCATTACCAATCACATCGCTGGCCCCTGCTGCCAGGGCAATCGCCTGGGAGTCGGGTTTCACCAGTGGATTAACCAGCCACAGAT > minE/1695783‑1695915 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |