| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,728,678 | T→C | 47.9% | F18F (TTT→TTC) | yfhD → | predicted transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,728,678 | 0 | T | C | 47.9% | 1.3 / 107.3 | 73 | F18F (TTT→TTC) | yfhD | predicted transglycosylase |
| Reads supporting (aligned to +/- strand): ref base T (12/26); new base C (18/17); total (30/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGACCGTGCGG > minE/1728614‑1728747 | gagaGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTg < 1:548067/71‑1 (MQ=255)gagaGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTg < 1:4125214/71‑1 (MQ=255)gagaGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTg < 1:1581463/71‑1 (MQ=255)gagaGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTg < 1:3129821/71‑1 (MQ=255)gagaGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTg < 1:300367/71‑1 (MQ=255)gagaGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTg < 1:2418947/71‑1 (MQ=255) agagTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTgg < 1:3246290/71‑1 (MQ=255) agagTTGCGTGTGAGCACAATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAGCCTTTCgg > 1:3260564/1‑66 (MQ=255) ttGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTa > 1:524117/1‑71 (MQ=255) ttGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTa > 1:3482462/1‑71 (MQ=255) gCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTg < 1:3365552/64‑1 (MQ=255) cGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAACCCTTTTGGCCTGGATTACg < 1:3512377/70‑1 (MQ=255) cGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACg < 1:4146258/70‑1 (MQ=255) cGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACg < 1:3024490/70‑1 (MQ=255) tgAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGa > 1:1291108/1‑61 (MQ=255) tgAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACt < 1:2784503/70‑1 (MQ=255) aGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGc > 1:2716073/1‑71 (MQ=255) gCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGc > 1:1898389/1‑70 (MQ=255) cACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTgg > 1:2296418/1‑51 (MQ=255) cACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGa < 1:3601053/71‑1 (MQ=255) aCCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGTCCTg < 1:141801/54‑1 (MQ=255) ccATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACt < 1:966318/64‑1 (MQ=255) ccATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACt < 1:3987432/64‑1 (MQ=255) cATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGAtt < 1:1583314/56‑1 (MQ=255) aTTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGaaa > 1:2668745/1‑69 (MQ=255) ttCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGa > 1:1664037/1‑52 (MQ=255) tCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACa > 1:4031493/1‑69 (MQ=255) tCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGa < 1:2994150/57‑1 (MQ=255) tCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGa < 1:3287670/57‑1 (MQ=255) cATTCTCCCCTGACTTATAACGAATTCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGtt > 1:1451311/1‑71 (MQ=255) tCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGa > 1:1651598/1‑45 (MQ=255) cccTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTg < 1:3233149/41‑1 (MQ=255) cccTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAt > 1:3219222/1‑71 (MQ=255) cTGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTa > 1:1945360/1‑71 (MQ=255) tGACTTATAACGAAATCAACGGGAAACCTTTCGGc < 1:2925469/35‑1 (MQ=255) tGACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGa < 1:3274296/54‑1 (MQ=255) gACTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTAcc > 1:1388928/1‑71 (MQ=255) aCTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTAc < 1:1280734/69‑1 (MQ=255) aCTTATAACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCt > 1:3379494/1‑71 (MQ=255) ataACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAt < 1:204931/62‑1 (MQ=255) ataACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTa > 1:341939/1‑64 (MQ=255) ataACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGaa > 1:1740872/1‑41 (MQ=255) ataACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTa > 1:649100/1‑64 (MQ=255) taACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTg < 1:347417/43‑1 (MQ=255) taACGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACt > 1:1934304/1‑42 (MQ=255) aaCGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGaa < 1:2306957/47‑1 (MQ=255) aaCGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGaa < 1:1683383/47‑1 (MQ=255) aCGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGt > 1:2145044/1‑70 (MQ=255) cGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTaa < 1:1632021/71‑1 (MQ=255) cGAAATCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAAc > 1:994486/1‑47 (MQ=255) gAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTaa < 1:2013282/70‑1 (MQ=255) aaTCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTaaaa > 1:2686764/1‑70 (MQ=255) aaTCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAAc > 1:3882445/1‑71 (MQ=255) aaTCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAAc > 1:1968815/1‑71 (MQ=255) aTCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACt < 1:1375410/71‑1 (MQ=255) aTCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACt < 1:502535/71‑1 (MQ=255) aTCAACGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACt > 1:1632506/1‑71 (MQ=255) tCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTaa < 1:1529686/66‑1 (MQ=255) aaCGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGa > 1:3933982/1‑70 (MQ=255) aaCGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGa < 1:1730494/70‑1 (MQ=255) aaCGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGa < 1:846882/70‑1 (MQ=255) aCGGGAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGaaa < 1:3427792/71‑1 (MQ=255) gggAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGt < 1:3461353/71‑1 (MQ=255) gggAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGt < 1:1999788/71‑1 (MQ=255) ggAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTg < 1:2865982/71‑1 (MQ=255) ggAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTg < 1:3548431/71‑1 (MQ=255) gAAACCTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGa > 1:912822/1‑71 (MQ=255) aaaCCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGAc < 1:3065745/71‑1 (MQ=255) aaCCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCg > 1:238910/1‑42 (MQ=255) aaCCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTaaaa < 1:1505293/59‑1 (MQ=255) aaCCTTTTGGCCTCGATTACGAACTGGCGAAACAGTTTGCCg < 1:64172/42‑1 (MQ=255) ccTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAt < 1:826061/42‑1 (MQ=255) ccTTTCGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGaaa > 1:611290/1‑63 (MQ=255) ttGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTaaaa > 1:4096556/1‑53 (MQ=255) ttGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGACCGTGCgg > 1:1943680/1‑71 (MQ=255) | GAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGTGACCGTGCGG > minE/1728614‑1728747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |