| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 223,016 | G→A | 14.9% | intergenic (+71/‑109) | lpcA → / → prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 223,016 | 0 | G | A | 14.9% | 188.0 / 20.1 | 67 | intergenic (+71/‑109) | lpcA/prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Reads supporting (aligned to +/- strand): ref base G (27/30); new base A (4/6); total (31/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCC > minE/222949‑223084 | tGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcg > 1:3618317/1‑71 (MQ=255)tGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcg > 1:3623050/1‑71 (MQ=255) gCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGa > 1:1322626/1‑67 (MQ=255) gCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAcgc < 1:413923/70‑1 (MQ=255) gCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGAc < 1:3192193/68‑1 (MQ=255) cGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGt > 1:238771/1‑71 (MQ=14) gTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTc < 1:1687108/71‑1 (MQ=255) tAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTa < 1:2568575/71‑1 (MQ=12) tAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTa > 1:2702424/1‑71 (MQ=12) aGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTa < 1:398636/70‑1 (MQ=12) ggCCGGATAAGGCGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTa < 1:2526099/71‑1 (MQ=11) ggCCGGATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCtt < 1:3840587/70‑1 (MQ=14) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCt < 1:1453856/68‑1 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTAt < 1:3147032/71‑1 (MQ=255) gCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTAt < 1:719785/71‑1 (MQ=255) ggATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCAt > 1:3875956/1‑71 (MQ=255) cGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACa > 1:2348861/1‑70 (MQ=255) cGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACa > 1:2312175/1‑70 (MQ=255) tttATGCCGCATCCGGCAGTAATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTAt < 1:3129315/58‑1 (MQ=11) tttACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACaaa < 1:1937058/70‑1 (MQ=255) tttACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAAt < 1:1172589/71‑1 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc < 1:680451/70‑1 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc > 1:3586635/1‑70 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc < 1:110868/70‑1 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCt > 1:3760552/1‑71 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCt > 1:3697971/1‑71 (MQ=255) tACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAttc > 1:1879168/1‑69 (MQ=11) cgccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTg < 1:20578/70‑1 (MQ=255) ccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTa < 1:1513807/70‑1 (MQ=255) gcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgc > 1:249184/1‑71 (MQ=255) cATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgc > 1:912032/1‑70 (MQ=255) aTCCGGCAGTCGTGTGCTGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:37824/48‑1 (MQ=14) cGGCATTTGTGCGCTGATGCCTGATGCGACGCTGa > 1:1050908/1‑35 (MQ=255) cGGCATTTGTGCGCTGATGCCTGATGCGACGCTGCCGCGTCTTATCATGCCTACAAATCTGTACGCGAAcc > 1:2331005/1‑71 (MQ=255) cGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAAcc > 1:3142606/1‑71 (MQ=255) ggCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg < 1:917430/71‑1 (MQ=255) ggCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg < 1:832762/71‑1 (MQ=255) ggCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg < 1:2405781/71‑1 (MQ=255) ggCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg > 1:2075294/1‑71 (MQ=255) tttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc < 1:418304/54‑1 (MQ=255) tttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTg < 1:2126657/56‑1 (MQ=255) tttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCATACAAATCTGTACGCGAACCGTAgg > 1:1900840/1‑71 (MQ=255) ttGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACaa < 1:2458922/50‑1 (MQ=11) ttGTGCGCAGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACaa < 1:1963291/50‑1 (MQ=11) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:2080797/1‑42 (MQ=14) gtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc < 1:3783617/70‑1 (MQ=255) gcgcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCg < 1:3882356/69‑1 (MQ=255) cgcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaat < 1:2928515/71‑1 (MQ=255) cgcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaa > 1:72883/1‑70 (MQ=255) tgatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGt < 1:1416463/48‑1 (MQ=255) atgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaataat < 1:1202693/69‑1 (MQ=255) atgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGc > 1:2373905/1‑71 (MQ=255) atgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGc > 1:2193495/1‑71 (MQ=255) tgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg < 1:1379248/55‑1 (MQ=255) tgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCg < 1:1940905/71‑1 (MQ=255) gcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGt > 1:3771337/1‑71 (MQ=255) gcctgatgcGACGCTAACGCGACTTATCATGCCTACa > 1:1237910/1‑37 (MQ=11) cctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:1022089/71‑1 (MQ=255) cctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:1202913/71‑1 (MQ=255) cctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:1570863/71‑1 (MQ=255) gatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaataa > 1:1806158/1‑62 (MQ=255) gatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaat < 1:1101179/60‑1 (MQ=255) atgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcg > 1:302684/1‑71 (MQ=255) tgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcgc > 1:1669211/1‑71 (MQ=255) cTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGt > 1:1703369/1‑58 (MQ=255) cTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcgc > 1:47448/1‑64 (MQ=255) cTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATcc > 1:79132/1‑71 (MQ=255) gACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTa > 1:4079423/1‑41 (MQ=255) | TGGCGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCC > minE/222949‑223084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |