| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,747,030 | C→T | 5.6% | intergenic (+114/+95) | srmB → / ← yfiE | ATP‑dependent RNA helicase/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,747,030 | 0 | C | T | 5.6% | 235.4 / 2.9 | 71 | intergenic (+114/+95) | srmB/yfiE | ATP‑dependent RNA helicase/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (25/42); new base T (2/2); total (27/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01 | |||||||||||
AAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAAT > minE/1746963‑1747099 | aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg < 1:1705711/71‑1 (MQ=255)aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg < 1:154733/71‑1 (MQ=255) aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg < 1:2087123/70‑1 (MQ=255) aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg < 1:996861/70‑1 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAg > 1:3672688/1‑71 (MQ=255) cTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAgg > 1:2490902/1‑71 (MQ=255) tGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg < 1:763461/66‑1 (MQ=255) tGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTa > 1:1160001/1‑68 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGcc > 1:1007775/1‑71 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGAt < 1:671991/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGAt < 1:3605692/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGAt < 1:2462306/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt < 1:3721914/70‑1 (MQ=14) cTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCg < 1:3979899/71‑1 (MQ=255) tACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGt > 1:428956/1‑71 (MQ=255) aCGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg > 1:3517398/1‑53 (MQ=255) aCGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt > 1:2921837/1‑71 (MQ=14) cGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTc < 1:1198617/71‑1 (MQ=255) cGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTc < 1:3459371/71‑1 (MQ=255) cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt < 1:2649708/70‑1 (MQ=14) gCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTa > 1:1034879/1‑53 (MQ=255) cTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg < 1:3115032/50‑1 (MQ=255) aTCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg < 1:3878535/69‑1 (MQ=255) tCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgccg < 1:1596953/71‑1 (MQ=255) aGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGt < 1:3085821/61‑1 (MQ=255) aGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCa > 1:1144103/1‑71 (MQ=255) aGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:2810920/71‑1 (MQ=255) aGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCa > 1:3702629/1‑71 (MQ=11) tACGTGGCTCATGCAATATATTGAATTTGCACGATCtt < 1:2325422/38‑1 (MQ=255) tACGTGGCTCATGCAATATATTGAATTTGCACGATCtt < 1:1999201/38‑1 (MQ=255) tACGTGGCTCATGCAATATATTGAATTTGCACGATCt < 1:2057335/37‑1 (MQ=255) tACGTGGCTCATGCAATATATTGAATTTGCACGATCt < 1:1617068/37‑1 (MQ=255) tACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGAt < 1:2649184/49‑1 (MQ=255) cGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa > 1:2896352/1‑49 (MQ=255) cGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa > 1:2978402/1‑49 (MQ=255) cGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:3837988/69‑1 (MQ=255) cGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc < 1:580446/70‑1 (MQ=255) cGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa > 1:3792565/1‑71 (MQ=255) gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTa > 1:1910464/1‑38 (MQ=255) tGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCg < 1:759210/42‑1 (MQ=255) gCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgccg > 1:792362/1‑58 (MQ=255) gCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa > 1:1902471/1‑71 (MQ=255) aTGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATa < 1:2955016/69‑1 (MQ=255) aTGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATa > 1:3990894/1‑69 (MQ=255) tGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAg < 1:3406973/71‑1 (MQ=255) cAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa > 1:421252/1‑38 (MQ=255) cAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:2937799/1‑55 (MQ=255) cAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa < 1:2650320/60‑1 (MQ=255) cAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAgcg < 1:261330/71‑1 (MQ=255) atatatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATaa < 1:2485958/65‑1 (MQ=255) atatatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAgcg < 1:2130416/69‑1 (MQ=255) tatatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATaa < 1:1206774/64‑1 (MQ=255) atatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:800510/1‑51 (MQ=38) atatAGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCa > 1:1405506/1‑42 (MQ=255) tatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACtt < 1:971010/71‑1 (MQ=255) tatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACt < 1:470337/70‑1 (MQ=255) atTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCa > 1:161987/1‑40 (MQ=40) ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACttt < 1:1492894/70‑1 (MQ=255) aTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGa > 1:2924759/1‑71 (MQ=255) tttGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGAc < 1:1293943/71‑1 (MQ=255) ttGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGAc > 1:1957860/1‑70 (MQ=255) tGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGa > 1:3144925/1‑68 (MQ=255) gCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACaa < 1:1149120/70‑1 (MQ=255) cGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:3713264/71‑1 (MQ=255) cGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:3575827/71‑1 (MQ=255) cGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:2088843/71‑1 (MQ=255) cGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:3751677/71‑1 (MQ=255) gATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGt > 1:1308222/1‑68 (MQ=255) gATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:4004541/70‑1 (MQ=255) aTCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:4055045/69‑1 (MQ=255) tCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAAt > 1:449857/1‑71 (MQ=255) | AAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAAT > minE/1746963‑1747099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |