| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,826,256 | G→A | 32.3% | A23V (GCG→GTG) | hydN ← | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,826,256 | 0 | G | A | 32.3% | 76.9 / 53.0 | 62 | A23V (GCG→GTG) | hydN | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base G (26/16); new base A (10/10); total (36/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 | |||||||||||
ACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAA > minE/1826187‑1826326 | aCATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACcgc > 1:3304531/1‑71 (MQ=255)aCATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACcgc > 1:2569633/1‑71 (MQ=255) aTGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCgcgc < 1:437912/71‑1 (MQ=255) gATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACg > 1:2184299/1‑71 (MQ=255) tACGCGGTAAAAAAGTTTCCGGGGTCAGCTATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCa < 1:3552862/67‑1 (MQ=255) tACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCt < 1:3196766/71‑1 (MQ=255) cgGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACtt < 1:843693/71‑1 (MQ=255) aaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAgg < 1:340263/71‑1 (MQ=255) aaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg > 1:158531/1‑71 (MQ=255) aaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg > 1:2277659/1‑71 (MQ=255) aaaGTTTCCGGGGTCAGCGATGAACAGTCCTGATTTTCCTGATGAGAAACAGCGCACGCTACTTCACAgg > 1:2851255/1‑70 (MQ=255) aGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTAc > 1:3113173/1‑71 (MQ=255) aGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAg > 1:4043734/1‑67 (MQ=255) tttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACg < 1:2726939/70‑1 (MQ=255) tttCCGGGGTCAGCGATGCACAGTCCTGA‑TTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACg < 1:110761/69‑1 (MQ=255) tCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGc < 1:1274855/70‑1 (MQ=255) tCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGc < 1:1558496/70‑1 (MQ=255) ggggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAAcc < 1:2179737/71‑1 (MQ=255) tCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCAc < 1:257502/44‑1 (MQ=255) tCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAAc < 1:2993132/66‑1 (MQ=255) aTGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCaa > 1:1007957/1‑59 (MQ=255) aTGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAAcc < 1:3346515/61‑1 (MQ=255) aTGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTAATTCACAGGTACGGCAACCAATACATTTa > 1:3282187/1‑71 (MQ=255) gCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTcaca > 1:2153449/1‑47 (MQ=255) gCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt > 1:591218/1‑71 (MQ=255) cacaGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCaa > 1:2279165/1‑60 (MQ=255) cacaGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTc > 1:1911597/1‑71 (MQ=255) acaGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTa > 1:92698/1‑38 (MQ=255) acaGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcg > 1:1977199/1‑71 (MQ=255) caatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACa > 1:2551913/4‑62 (MQ=255) aGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATAc < 1:3663530/60‑1 (MQ=255) tCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACgg > 1:1371467/1‑48 (MQ=255) tCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGt > 1:2465707/1‑70 (MQ=255) ccTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATc > 1:2025834/1‑70 (MQ=255) cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCa > 1:1776352/1‑52 (MQ=255) cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg > 1:3722088/1‑71 (MQ=255) cTGATTTTCCTGATGAGAAACCACTCACGCTACTTCACAGGTACgg > 1:196872/1‑46 (MQ=39) cTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGc > 1:3217621/1‑47 (MQ=255) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATccg > 1:418110/1‑69 (MQ=255) tGATTTTCCTGATGAGTAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCa < 1:3630492/69‑1 (MQ=255) tGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCaa > 1:2706893/1‑52 (MQ=255) tGATTTTCCTGATGAGAAACCGCGAACGCTACTTCACAGGTACGGCAACCAATACAttt < 1:178181/59‑1 (MQ=255) gATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACAtt > 1:2293343/1‑57 (MQ=255) gATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTa < 1:2537935/59‑1 (MQ=255) gATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc > 1:2662702/1‑70 (MQ=255) gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTa < 1:2375934/59‑1 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:3131823/69‑1 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:4064495/69‑1 (MQ=255) ttttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAat < 1:1333237/71‑1 (MQ=255) tttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAat > 1:2002547/1‑70 (MQ=255) tttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAat > 1:246115/1‑70 (MQ=255) ttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatg < 1:1890953/70‑1 (MQ=255) cTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAAtgatga < 1:3990270/71‑1 (MQ=255) tgatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATa < 1:585440/45‑1 (MQ=255) gatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATa > 1:3579189/1‑44 (MQ=16) agaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa > 1:1496109/1‑67 (MQ=255) agaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:251758/1‑71 (MQ=255) agaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:2057456/1‑71 (MQ=255) gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:811174/1‑71 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat < 1:1169741/61‑1 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:1822717/1‑70 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:2894175/1‑70 (MQ=255) aaaCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtc < 1:3534467/71‑1 (MQ=255) aCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat > 1:3215940/1‑71 (MQ=255) ctgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCg < 1:557322/62‑1 (MQ=255) ccGCGCACGCTACTTCACAGGTACGGCAACCAATACAtt < 1:4057081/39‑1 (MQ=255) cgcgCACGCTACTTCACAGGTACGGCAACCAATACAtt < 1:3038027/38‑1 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatgat > 1:2577913/1‑68 (MQ=255) | ACATGGATACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAA > minE/1826187‑1826326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |