| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,826,264 | T→C | 9.7% | V20V (GTA→GTG) | hydN ← | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,826,264 | 0 | T | C | 9.7% | 197.1 / 11.2 | 72 | V20V (GTA→GTG) | hydN | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base T (36/29); new base C (5/2); total (41/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTC > minE/1826194‑1826332 | tACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCt < 1:3196766/71‑1 (MQ=255) cgGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACtt < 1:843693/71‑1 (MQ=255) aaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAgg < 1:340263/71‑1 (MQ=255) aaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg > 1:2277659/1‑71 (MQ=255) aaaaGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAgg > 1:158531/1‑71 (MQ=255) aaaGTTTCCGGGGTCAGCGATGAACAGTCCTGATTTTCCTGATGAGAAACAGCGCACGCTACTTCACAgg > 1:2851255/1‑70 (MQ=255) aGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTAc > 1:3113173/1‑71 (MQ=255) aGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAg > 1:4043734/1‑67 (MQ=255) tttCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACg < 1:2726939/70‑1 (MQ=255) tttCCGGGGTCAGCGATGCACAGTCCTGA‑TTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACg < 1:110761/69‑1 (MQ=255) tCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGc < 1:1558496/70‑1 (MQ=255) tCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGc < 1:1274855/70‑1 (MQ=255) ggggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAAcc < 1:2179737/71‑1 (MQ=255) tCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAAc < 1:2993132/66‑1 (MQ=255) aTGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCaa > 1:1007957/1‑59 (MQ=255) aTGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAAcc < 1:3346515/61‑1 (MQ=255) aTGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTAATTCACAGGTACGGCAACCAATACATTTa > 1:3282187/1‑71 (MQ=255) gCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTcaca > 1:2153449/1‑47 (MQ=255) gCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt > 1:591218/1‑71 (MQ=255) cacaGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCaa > 1:2279165/1‑60 (MQ=255) cacaGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTc > 1:1911597/1‑71 (MQ=255) acaGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTa > 1:92698/1‑38 (MQ=255) acaGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcg > 1:1977199/1‑71 (MQ=255) caatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACa > 1:2551913/4‑62 (MQ=255) aGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATAc < 1:3663530/60‑1 (MQ=255) tCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACgg > 1:1371467/1‑48 (MQ=255) tCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGt > 1:2465707/1‑70 (MQ=255) ccTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATc > 1:2025834/1‑70 (MQ=255) cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCa > 1:1776352/1‑52 (MQ=255) cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg > 1:3722088/1‑71 (MQ=255) cTGATTTTCCTGATGAGAAACCACTCACGCTACTTCACAGGTACgg > 1:196872/1‑46 (MQ=39) cTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGc > 1:3217621/1‑47 (MQ=255) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATccg > 1:418110/1‑69 (MQ=255) tGATTTTCCTGATGAGTAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCa < 1:3630492/69‑1 (MQ=255) tGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCaa > 1:2706893/1‑52 (MQ=255) tGATTTTCCTGATGAGAAACCGCGAACGCTACTTCACAGGTACGGCAACCAATACAttt < 1:178181/59‑1 (MQ=255) gATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACAtt > 1:2293343/1‑57 (MQ=255) gATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTa < 1:2537935/59‑1 (MQ=255) gATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc > 1:2662702/1‑70 (MQ=255) gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTa < 1:2375934/59‑1 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:4064495/69‑1 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:3131823/69‑1 (MQ=255) ttttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAat < 1:1333237/71‑1 (MQ=255) tttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAat > 1:2002547/1‑70 (MQ=255) tttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAat > 1:246115/1‑70 (MQ=255) ttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatg < 1:1890953/70‑1 (MQ=255) cTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAAtgatga < 1:3990270/71‑1 (MQ=255) tgatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATa < 1:585440/45‑1 (MQ=255) gatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATa > 1:3579189/1‑44 (MQ=16) agaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa > 1:1496109/1‑67 (MQ=255) agaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:251758/1‑71 (MQ=255) agaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:2057456/1‑71 (MQ=255) gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:811174/1‑71 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat < 1:1169741/61‑1 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:2894175/1‑70 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:1822717/1‑70 (MQ=255) aaaCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtc < 1:3534467/71‑1 (MQ=255) aCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat > 1:3215940/1‑71 (MQ=255) ctgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCg < 1:557322/62‑1 (MQ=255) ccGCGCACGCTACTTCACAGGTACGGCAACCAATACAtt < 1:4057081/39‑1 (MQ=255) cgcgCACGCTACTTCACAGGTACGGCAACCAATACAtt < 1:3038027/38‑1 (MQ=255) cacgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat > 1:1757785/3‑69 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatgat > 1:2577913/1‑68 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg < 1:1236999/48‑1 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:3502761/71‑1 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:801564/71‑1 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:1422001/71‑1 (MQ=255) cACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAt > 1:1935472/1‑70 (MQ=255) aCGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc < 1:3257930/71‑1 (MQ=255) aCGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc < 1:2618879/71‑1 (MQ=255) cGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:1075433/1‑60 (MQ=255) cTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtctc > 1:3251339/1‑70 (MQ=255) cTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtctc > 1:2036722/1‑70 (MQ=255) | TACGCGGTAAAAAAGTTTCCGGGGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTC > minE/1826194‑1826332 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |