| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,826,295 | C→G | 6.0% | S10T (AGT→ACT) ‡ | hydN ← | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,826,295 | 0 | C | G | 6.0% | 314.0 / 7.3 | 100 | S10T (AGT→ACT) ‡ | hydN | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base C (47/47); new base G (3/3); total (50/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATT > minE/1826226‑1826362 | gCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt > 1:591218/1‑71 (MQ=255) cacaGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTc > 1:1911597/1‑71 (MQ=255) acaGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTcg > 1:1977199/1‑71 (MQ=255) tCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGt > 1:2465707/1‑70 (MQ=255) ccTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATc > 1:2025834/1‑70 (MQ=255) cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg > 1:3722088/1‑71 (MQ=255) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATccg > 1:418110/1‑69 (MQ=255) tGATTTTCCTGATGAGTAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCa < 1:3630492/69‑1 (MQ=255) gATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc > 1:2662702/1‑70 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:3131823/69‑1 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGc < 1:4064495/69‑1 (MQ=255) ttttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAat < 1:1333237/71‑1 (MQ=255) tttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAat > 1:2002547/1‑70 (MQ=255) tttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAat > 1:246115/1‑70 (MQ=255) ttCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatg < 1:1890953/70‑1 (MQ=255) cTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAAtgatga < 1:3990270/71‑1 (MQ=255) agaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa > 1:1496109/1‑67 (MQ=255) agaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:2057456/1‑71 (MQ=255) agaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:251758/1‑71 (MQ=255) gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:811174/1‑71 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat < 1:1169741/61‑1 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:1822717/1‑70 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:2894175/1‑70 (MQ=255) aaaCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtc < 1:3534467/71‑1 (MQ=255) aCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat > 1:3215940/1‑71 (MQ=255) ctgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCg < 1:557322/62‑1 (MQ=255) cacgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcat > 1:1757785/3‑69 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatgat > 1:2577913/1‑68 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg < 1:1236999/48‑1 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:1422001/71‑1 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:801564/71‑1 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:3502761/71‑1 (MQ=255) cACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAt > 1:1935472/1‑70 (MQ=255) aCGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc < 1:2618879/71‑1 (MQ=255) aCGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc < 1:3257930/71‑1 (MQ=255) cGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:1075433/1‑60 (MQ=255) cTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtctc > 1:2036722/1‑70 (MQ=255) cTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtctc > 1:3251339/1‑70 (MQ=255) cTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaa < 1:4052453/49‑1 (MQ=255) cTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc < 1:2263923/71‑1 (MQ=255) cTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc < 1:3977468/71‑1 (MQ=255) ttCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtc > 1:2956082/1‑55 (MQ=255) ttCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:2319128/61‑1 (MQ=255) ttCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt > 1:590801/1‑63 (MQ=255) tCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGtt > 1:3552746/1‑53 (MQ=255) tCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCaa > 1:4039486/1‑71 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCaa > 1:480184/1‑39 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt > 1:1071979/1‑61 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt > 1:2890239/1‑71 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt > 1:3038849/1‑71 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt > 1:3603030/1‑71 (MQ=255) acaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt < 1:4127598/70‑1 (MQ=255) caGGTACGGCAACCAATACATTTACTCGCGTCAGCAat < 1:3373001/38‑1 (MQ=255) aGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc < 1:1331248/65‑1 (MQ=255) ggTACGGCAACCAATACATTTACTCGCGTCAGCAatga > 1:2684685/1‑38 (MQ=255) ggTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGa > 1:1778917/1‑69 (MQ=255) gTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGtt < 1:2059328/47‑1 (MQ=255) gTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatg < 1:1142972/51‑2 (MQ=255) gTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc < 1:2016493/63‑1 (MQ=255) aCGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCaa > 1:3891692/1‑63 (MQ=255) aCGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt > 1:134986/1‑70 (MQ=255) aCGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt > 1:3552096/1‑70 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt < 1:114183/70‑1 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt < 1:1360334/70‑1 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTa < 1:1928037/71‑1 (MQ=255) ggCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc < 1:1547761/59‑1 (MQ=255) ggCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt < 1:1364714/69‑1 (MQ=255) gCAACCAATACATTTACTCGCGTCATCAATGATGAAACGGTtc < 1:2828109/43‑1 (MQ=255) gCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt > 1:3132986/1‑67 (MQ=255) cAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATg > 1:3924388/1‑71 (MQ=255) aCCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtct < 1:1212917/50‑1 (MQ=255) aCCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtct < 1:401742/50‑1 (MQ=255) aCCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGa < 1:84619/60‑1 (MQ=255) aaTACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:586277/43‑1 (MQ=255) aaTACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGcc > 1:3762392/1‑70 (MQ=255) aTACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCg < 1:1934691/70‑1 (MQ=255) tACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:633361/41‑1 (MQ=255) tACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt < 1:3141865/53‑1 (MQ=255) tACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGcc < 1:2703121/68‑1 (MQ=255) tACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGcc < 1:2211907/68‑1 (MQ=255) tACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGa < 1:617924/70‑1 (MQ=255) cATTTACTCGCGTCAGCAATGATGAAACGGTtcat < 1:1615979/35‑1 (MQ=255) cATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa > 1:3534248/1‑70 (MQ=255) cATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa > 1:1297471/1‑70 (MQ=255) cATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa > 1:3979096/1‑70 (MQ=255) cATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa < 1:109080/70‑1 (MQ=255) cATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa > 1:1899272/1‑70 (MQ=255) cATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa > 1:3552818/1‑70 (MQ=255) tttACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:4124156/37‑1 (MQ=255) tttACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:592186/37‑1 (MQ=255) tttACTCGCGTCAGCAATGATGAAACGGTTCATCaaa < 1:1778666/37‑1 (MQ=255) tttACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc < 1:874887/40‑1 (MQ=255) tttACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc < 1:1641240/40‑1 (MQ=255) tttACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGa < 1:2147737/51‑1 (MQ=255) tttACTAGCGTCAGCAATGATGAAACGGTtcatca > 1:2207357/1‑35 (MQ=255) tACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt > 1:1341825/1‑54 (MQ=255) tACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATa > 1:2428755/1‑66 (MQ=255) tACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACAtt > 1:752452/1‑70 (MQ=255) tACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACAtt > 1:1098902/1‑70 (MQ=255) cTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc < 1:2878303/42‑1 (MQ=255) | GCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATT > minE/1826226‑1826362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |