| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,837,432 | G→A | 6.5% | N329N (AAC→AAT) | nlpD ← | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,837,432 | 0 | G | A | 6.5% | 300.2 / 7.7 | 92 | N329N (AAC→AAT) | nlpD | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (45/41); new base A (2/4); total (47/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
GGTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAG > minE/1837363‑1837500 | ggTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGt < 1:1984598/71‑1 (MQ=255)ggTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGt < 1:874745/71‑1 (MQ=255)ggTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGt < 1:351873/71‑1 (MQ=255)ggTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGt < 1:3164717/71‑1 (MQ=255) gTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGtt < 1:3957794/71‑1 (MQ=255) cTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTAt < 1:2097388/70‑1 (MQ=255) ccATGGTCGGTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCg < 1:3266650/71‑1 (MQ=255) ccATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCg > 1:3720986/1‑71 (MQ=255) cATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGt < 1:3460288/71‑1 (MQ=255) aTGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGtt < 1:3214290/62‑1 (MQ=255) tGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGtt < 1:2529393/61‑1 (MQ=255) gTCGATATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAgg > 1:1301271/1‑70 (MQ=255) tCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGt < 1:663958/57‑1 (MQ=255) tcTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGtt < 1:2504401/55‑1 (MQ=255) gCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:958372/1‑57 (MQ=255) gCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAgg > 1:1903038/1‑67 (MQ=255) gCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACt < 1:1794422/71‑1 (MQ=255) gCTATTTTTTGCCCCGCCTTAA‑‑‑CTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:3144419/1‑54 (MQ=255) cTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTc < 1:3227877/71‑1 (MQ=255) cTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTc < 1:212305/71‑1 (MQ=255) tATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa < 1:1241812/55‑1 (MQ=255) aTTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAg > 1:2241295/1‑71 (MQ=255) aTTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAg > 1:1856763/1‑71 (MQ=255) ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:3635703/1‑53 (MQ=255) tttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:887479/1‑52 (MQ=255) tttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAgg > 1:1710542/1‑70 (MQ=255) ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTAt > 1:3082750/1‑52 (MQ=255) gCCCCGCCTTAACTTCTAGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTaa < 1:3050616/68‑1 (MQ=255) ccccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatca < 1:640369/70‑1 (MQ=255) cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGt < 1:1384943/41‑1 (MQ=255) cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:1879163/1‑43 (MQ=255) gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:2630090/1‑70 (MQ=255) ccTTAACTTCTTGTTGTTCCCTGACCAGCATTGTGTCGt < 1:1507818/39‑1 (MQ=255) ccTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGt < 1:2753682/39‑1 (MQ=255) ttAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGt < 1:3431919/37‑1 (MQ=255) ttAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:974885/1‑39 (MQ=255) ttAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa > 1:2416015/1‑39 (MQ=255) ttAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGt < 1:3783806/46‑1 (MQ=255) ttAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTa < 1:302491/47‑1 (MQ=255) ttAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa > 1:977391/1‑69 (MQ=255) tAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGt < 1:2210528/45‑1 (MQ=255) tAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:1026135/1‑66 (MQ=255) tAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt > 1:1922686/1‑71 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:2198573/1‑71 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:1694774/1‑71 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:3200491/1‑71 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:2833653/1‑71 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:2712619/1‑71 (MQ=255) aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa < 1:1210591/36‑1 (MQ=255) cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACt < 1:258571/49‑1 (MQ=255) cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAg > 1:3804242/1‑52 (MQ=255) tgttgtTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtg > 1:2977308/1‑66 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCATGTAATCATCATTATGTTtgatga < 1:2130903/69‑1 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga < 1:2880654/69‑1 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat > 1:1436407/1‑70 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat > 1:4082354/1‑70 (MQ=255) ttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATaa < 1:1072264/71‑1 (MQ=255) ttgtt‑CCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATaa < 1:77653/70‑1 (MQ=255) gttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:2521679/1‑54 (MQ=255) gttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATa < 1:3941138/68‑1 (MQ=255) gttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt < 1:3212871/70‑1 (MQ=255) ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa < 1:3954538/71‑1 (MQ=255) tCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCa > 1:1515143/1‑36 (MQ=255) tCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCa > 1:3789843/1‑40 (MQ=255) cccGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTaa > 1:964700/1‑44 (MQ=255) cccGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa > 1:2299161/1‑43 (MQ=255) cGGACCAGCATTGTGTCGTTATGGGCGTAGGCACt < 1:2448293/35‑1 (MQ=255) ccAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa < 1:1624757/63‑1 (MQ=255) ccAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg > 1:1733518/1‑71 (MQ=255) ccAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg > 1:2241509/1‑71 (MQ=255) cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg > 1:2681483/1‑70 (MQ=255) cATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAcc > 1:2130067/1‑66 (MQ=255) cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc < 1:4123061/71‑1 (MQ=255) atcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc < 1:102012/67‑1 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatg < 1:2810053/50‑1 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg > 1:223673/1‑68 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg > 1:3613809/1‑71 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg > 1:690536/1‑71 (MQ=255) tcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAc < 1:4132440/61‑1 (MQ=255) gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt > 1:2922585/1‑44 (MQ=255) gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat > 1:1564132/1‑47 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga > 1:1107573/1‑57 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga > 1:618454/1‑57 (MQ=255) tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCacgc < 1:447301/71‑1 (MQ=255) gtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:2530778/1‑41 (MQ=255) gtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg > 1:1399836/1‑69 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATg < 1:3982061/38‑1 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt < 1:256968/39‑1 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg > 1:3758102/1‑71 (MQ=255) cGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt < 1:1470513/38‑1 (MQ=255) cGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt < 1:3245449/38‑1 (MQ=255) cGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAcc > 1:3222141/1‑58 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:1278284/1‑38 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:874666/1‑38 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAcc < 1:2710619/57‑1 (MQ=255) | GGTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAG > minE/1837363‑1837500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |