| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,837,452 | G→T | 12.3% | L323M (CTG→ATG) ‡ | nlpD ← | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,837,452 | 0 | G | T | 12.3% | 224.4 / 9.9 | 72 | L323M (CTG→ATG) ‡ | nlpD | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (38/25); new base T (2/7); total (40/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.82e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
ATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACG > minE/1837382‑1837522 | aTTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAg > 1:2241295/1‑71 (MQ=255)aTTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAg > 1:1856763/1‑71 (MQ=255) tttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAgg > 1:1710542/1‑70 (MQ=255) gCCCCGCCTTAACTTCTAGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTaa < 1:3050616/68‑1 (MQ=255) ccccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatca < 1:640369/70‑1 (MQ=255) gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:2630090/1‑70 (MQ=255) ttAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa > 1:977391/1‑69 (MQ=255) tAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:1026135/1‑66 (MQ=255) tAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt > 1:1922686/1‑71 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:2712619/1‑71 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:3200491/1‑71 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:2833653/1‑71 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:1694774/1‑71 (MQ=255) aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:2198573/1‑71 (MQ=255) cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAg > 1:3804242/1‑52 (MQ=255) tgttgtTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtg > 1:2977308/1‑66 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCATGTAATCATCATTATGTTtgatga < 1:2130903/69‑1 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga < 1:2880654/69‑1 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat > 1:4082354/1‑70 (MQ=255) gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat > 1:1436407/1‑70 (MQ=255) ttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATaa < 1:1072264/71‑1 (MQ=255) ttgtt‑CCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATaa < 1:77653/70‑1 (MQ=255) gttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat > 1:2521679/1‑54 (MQ=255) gttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATa < 1:3941138/68‑1 (MQ=255) gttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt < 1:3212871/70‑1 (MQ=255) ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa < 1:3954538/71‑1 (MQ=255) cccGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTaa > 1:964700/1‑44 (MQ=255) cccGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa > 1:2299161/1‑43 (MQ=255) ccAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa < 1:1624757/63‑1 (MQ=255) ccAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg > 1:2241509/1‑71 (MQ=255) ccAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg > 1:1733518/1‑71 (MQ=255) cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg > 1:2681483/1‑70 (MQ=255) cATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAcc > 1:2130067/1‑66 (MQ=255) cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc < 1:4123061/71‑1 (MQ=255) atcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc < 1:102012/67‑1 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatg < 1:2810053/50‑1 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg > 1:223673/1‑68 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg > 1:3613809/1‑71 (MQ=255) ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg > 1:690536/1‑71 (MQ=255) tcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAc < 1:4132440/61‑1 (MQ=255) gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt > 1:2922585/1‑44 (MQ=255) gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat > 1:1564132/1‑47 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga > 1:618454/1‑57 (MQ=255) gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga > 1:1107573/1‑57 (MQ=255) tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCacgc < 1:447301/71‑1 (MQ=255) gtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:2530778/1‑41 (MQ=255) gtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg > 1:1399836/1‑69 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATg < 1:3982061/38‑1 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt < 1:256968/39‑1 (MQ=255) tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg > 1:3758102/1‑71 (MQ=255) cGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt < 1:3245449/38‑1 (MQ=255) cGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt < 1:1470513/38‑1 (MQ=255) cGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAcc > 1:3222141/1‑58 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:874666/1‑38 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt > 1:1278284/1‑38 (MQ=255) gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAcc < 1:2710619/57‑1 (MQ=255) attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgct < 1:3853165/70‑2 (MQ=255) attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgct < 1:218434/70‑2 (MQ=255) ttATGGGCGTAGGCACTCAGGTAATCATCATTATGttt < 1:172997/38‑1 (MQ=255) ttATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcgc < 1:375140/71‑1 (MQ=255) aTGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt > 1:1332449/1‑45 (MQ=255) aTGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt > 1:3650222/1‑71 (MQ=255) aTGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt > 1:3501462/1‑71 (MQ=255) aTGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt > 1:1492891/1‑71 (MQ=255) tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg < 1:2810475/67‑1 (MQ=255) tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGtt < 1:971080/71‑1 (MQ=255) tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGtt < 1:2535124/71‑1 (MQ=255) ggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt < 1:973303/42‑1 (MQ=255) ggCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCa > 1:2378536/1‑70 (MQ=255) gCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa < 1:2449639/71‑1 (MQ=255) aCTCAGGTAATCATCATTATGTTTGATGATAATCAg < 1:3845218/36‑1 (MQ=255) tCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac > 1:2795219/1‑70 (MQ=255) cAGGTAATCATCATTATGTTTGATGATAATCAGAt > 1:3090907/1‑35 (MQ=255) cAGGTAATCATCATTATGTTTGATGATAATCAGAt > 1:2298775/1‑35 (MQ=255) aGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa < 1:2809451/70‑1 (MQ=255) aGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa < 1:3012238/70‑1 (MQ=255) ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAAcg < 1:1719454/71‑1 (MQ=255) | ATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACG > minE/1837382‑1837522 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |