| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,864,336 | G→A | 12.2% | intergenic (+83/+65) | ygcP → / ← ygcQ | predicted anti‑terminator regulatory protein/predicted flavoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,864,336 | 0 | G | A | 12.2% | 230.1 / 18.9 | 82 | intergenic (+83/+65) | ygcP/ygcQ | predicted anti‑terminator regulatory protein/predicted flavoprotein |
| Reads supporting (aligned to +/- strand): ref base G (45/27); new base A (6/4); total (51/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01 | |||||||||||
ACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTT > minE/1864267‑1864402 | aCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa > 1:3655392/1‑71 (MQ=255) cAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcg > 1:1812785/1‑71 (MQ=255) aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgc > 1:889229/1‑71 (MQ=255) aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgc < 1:3915297/71‑1 (MQ=255) aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgc > 1:183237/1‑71 (MQ=255) aTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgcg > 1:3412795/1‑71 (MQ=255) ttGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgc < 1:1264782/69‑1 (MQ=255) ttGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGt < 1:3197546/71‑1 (MQ=255) ttGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGt > 1:2380230/1‑71 (MQ=255) gtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGt > 1:675455‑M1/48‑71 (MQ=255) tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTc > 1:244389/1‑71 (MQ=255) gattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTc > 1:2399343‑M1/46‑70 (MQ=255) gattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTc < 1:3374261‑M1/25‑1 (MQ=255) gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTc < 1:2284437/70‑1 (MQ=255) gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCt > 1:3083328/1‑71 (MQ=255) attttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCt > 1:1847499‑M1/45‑70 (MQ=255) attttgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCtt > 1:2767252‑M1/45‑71 (MQ=255) aaTTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCtt < 1:1233038/71‑1 (MQ=255) aTTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgc > 1:736332/1‑65 (MQ=255) aTTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCtt < 1:3691480/70‑1 (MQ=255) aTTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTa < 1:3628077/71‑1 (MQ=255) tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGt > 1:3517401/1‑66 (MQ=255) tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTa > 1:2067448/1‑70 (MQ=255) tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt > 1:3749855/1‑71 (MQ=255) tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt > 1:2289927/1‑71 (MQ=255) tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt < 1:684465/71‑1 (MQ=255) ttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTAt < 1:3291702‑M1/29‑1 (MQ=255) ttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTc > 1:786558/1‑66 (MQ=255) ggTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa < 1:266688/58‑1 (MQ=255) ggTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt > 1:921810/1‑68 (MQ=255) taggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAg > 1:2123991‑M1/39‑70 (MQ=255) gTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg < 1:783991/70‑1 (MQ=255) tttGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt < 1:1006433/66‑1 (MQ=255) ttGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGcc > 1:3014361/1‑71 (MQ=255) ggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGcc < 1:3522756‑M1/35‑1 (MQ=255) tGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGcc > 1:3030767/1‑70 (MQ=255) cggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc < 1:1276829‑M1/38‑1 (MQ=255) gataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc > 1:3671455‑M1/32‑69 (MQ=255) ataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc < 1:1943384‑M1/40‑3 (MQ=255) gTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg > 1:1918647/1‑61 (MQ=255) tttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt > 1:2150327‑M1/23‑58 (MQ=255) tttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTacg > 1:654835‑M1/23‑60 (MQ=255) tacgccgcaaccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCt < 1:993478‑M1/26‑1 (MQ=255) aCGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa > 1:3223308/1‑39 (MQ=255) ccACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAgag > 1:4143059/1‑49 (MQ=255) aTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAAc > 1:998385/1‑69 (MQ=255) aTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg > 1:1853075/1‑71 (MQ=255) aTCCGACATTCAGCCCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTccc > 1:2534839/1‑58 (MQ=255) tCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCtt < 1:1604680/39‑1 (MQ=255) tCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg > 1:2816287/1‑70 (MQ=255) tCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg > 1:2074387/1‑70 (MQ=255) tccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTa > 1:1285918‑M1/12‑39 (MQ=255) tccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTa > 1:18329‑M1/12‑39 (MQ=255) ccGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt > 1:3093080/1‑40 (MQ=255) ccGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGa < 1:1149346/65‑1 (MQ=255) ccGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg > 1:2861536/1‑69 (MQ=255) ccGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg > 1:3902696/1‑69 (MQ=255) cGACATTCAGCGCCTGATGCGACGCTTGACGCGTCtt < 1:2069277/37‑1 (MQ=255) cGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAg > 1:2500803/1‑71 (MQ=255) gACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg > 1:3570257/1‑71 (MQ=255) aCATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg > 1:4106435/1‑70 (MQ=255) aTTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAg < 1:429319/71‑1 (MQ=255) ttCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAgg < 1:3127818/71‑1 (MQ=255) tCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGa > 1:647977/1‑71 (MQ=255) cAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGa < 1:2363599/70‑1 (MQ=255) gcgcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatca < 1:2094197/71‑1 (MQ=255) gcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCa > 1:1542726/1‑46 (MQ=40) tGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAg < 1:3818877/61‑1 (MQ=255) tGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAg < 1:1783208/61‑1 (MQ=255) aTGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGc > 1:958563/1‑70 (MQ=255) aTGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGc > 1:344903/1‑70 (MQ=255) tGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCGCAGGCCAGAACCGTAGGCAGGatca < 1:4072608/63‑1 (MQ=255) gCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCGGGATCATCCGGCaa > 1:1316916/1‑70 (MQ=255) gCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCaa > 1:541131/1‑70 (MQ=255) gCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCaa > 1:1825670/1‑70 (MQ=255) gACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAacac > 1:2590887/1‑71 (MQ=255) aCGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAAcc < 1:2212714/46‑1 (MQ=255) aCGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAacac < 1:1715271/70‑1 (MQ=255) cGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt < 1:2476790/71‑1 (MQ=255) cGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt > 1:281553/1‑71 (MQ=255) gCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGtt < 1:1837918/71‑1 (MQ=255) gCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt > 1:27855/1‑70 (MQ=255) | ACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTT > minE/1864267‑1864402 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |