BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,870,726	T→G	6.3%	L218W (TTG→TGG)	yqcE →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,870,726	0	T	G	6.3%	248.0 / 12.6	95	L218W (TTG→TGG)	yqcE	predicted transporter
Reads supporting (aligned to +/- strand): ref base T (52/37); new base G (2/4); total (54/41)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

Only 100 of 113 total aligned reads displayed.

AACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTGAG  >  minE/1870656‑1870795

aacaacaacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGtttt                                                                       >  1:1655312/1‑71 (MQ=255)

aacaacaacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGAAATCCTGGCCGtttt                                                                       >  1:1490791/1‑71 (MQ=255)

  caacaacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGtttt                                                                       <  1:2770620/69‑1 (MQ=255)

    acaacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGt                                                                   <  1:3877767/71‑1 (MQ=255)

      aacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTa                                                                  >  1:2906127/1‑70 (MQ=255)

      aacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTAt                                                                 >  1:1577520/1‑71 (MQ=255)

      aacCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTAt                                                                 >  1:1469507/1‑71 (MQ=255)

       acCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTAt                                                                 >  1:2122927/1‑70 (MQ=255)

       acCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTAt                                                                 >  1:3331862/1‑70 (MQ=255)

       acCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATc                                                                <  1:800679/71‑1 (MQ=255)

        ccTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTAt                                                                 >  1:1799557/1‑69 (MQ=255)

        ccTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATc                                                                >  1:2808525/1‑70 (MQ=255)

        ccTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTGGCGTa                                                                  >  1:3271514/1‑68 (MQ=255)

          tGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAg                                                              >  1:104128/1‑70 (MQ=255)

           gcgcAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGca                                                            >  1:3594379/1‑71 (MQ=255)

            cgcAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGc                                                             <  1:3128333/69‑1 (MQ=255)

             gcAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGcac                                                           >  1:2716564/1‑70 (MQ=255)

               aGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGcaccac                                                        <  1:4138842/71‑1 (MQ=255)

                 tGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCg                                                                    <  1:641316/57‑1 (MQ=255)

                  gCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTg                                                     >  1:615508/1‑71 (MQ=255)

                  gCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTg                                                     >  1:1403689/1‑71 (MQ=255)

                  gCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTg                                                     >  1:499051/1‑71 (MQ=255)

                   ccAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTgg                                                    <  1:3422984/71‑1 (MQ=255)

                   ccAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTgg                                                    >  1:1661784/1‑71 (MQ=255)

                   ccAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTg                                                     >  1:789003/1‑70 (MQ=255)

                      aTAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTa                                                                  <  1:1250044/54‑1 (MQ=255)

                      aTAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGt                                                   <  1:3262329/69‑1 (MQ=255)

                       tAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTa                                                                  <  1:650888/53‑1 (MQ=255)

                       tAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGcacc                                                          <  1:3560479/61‑1 (MQ=255)

                        aaCGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTAt                                                                 >  1:1330189/1‑53 (MQ=255)

                        aaCGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTAttt                                               >  1:1912155/1‑70 (MQ=255)

                        aaCGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTg                                               >  1:1941715/1‑71 (MQ=255)

                        aaCGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTg                                               >  1:2435838/1‑71 (MQ=255)

                         aCGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCg                                                                    >  1:858777/1‑49 (MQ=255)

                         aCGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATc                                                                >  1:2741664/1‑53 (MQ=255)

                         aCGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGcaccac                                                        >  1:2504843/1‑61 (MQ=255)

                         aCGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTGGCGTATCAGCACCACCTGGTATTgc                                              <  1:2602510/71‑1 (MQ=255)

                         aCGAAGAAAAACAGTCATTCCAGCTTAGCAACATCCTGGCCGTTTTGCGTATCAGc                                                             <  1:2069340/56‑1 (MQ=255)

                           gaagaaAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGcac                                                           >  1:3740290/1‑56 (MQ=255)

                           gaagaaAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGca                                                            >  1:1929265/1‑55 (MQ=255)

                             agaaAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGcaccac                                                        <  1:2832720/57‑1 (MQ=255)

                                aaaaCAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGcacca                                                         >  1:1307851/1‑53 (MQ=255)

                                 aaaCAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATc                                                                >  1:756853/1‑45 (MQ=255)

                                 aaaCAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGt                                      >  1:2882293/1‑71 (MQ=255)

                                  aaCAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGt                                      >  1:3348308/1‑70 (MQ=255)

                                    cAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTa                                                  >  1:799608/1‑56 (MQ=255)

                                     aGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGAtt                                  <  1:2043758/71‑1 (MQ=255)

                                     aGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGAtt                                  <  1:387791/71‑1 (MQ=255)

                                     aGTCATTCCAGCTTAGCGACATCCTAGCCGTTTTGCGTATCATCACCACCTGGTATTGCAGCATGGTGAtt                                  <  1:1403400/71‑1 (MQ=255)

                                       tCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTAt                                                                 <  1:3614346/38‑1 (MQ=255)

                                       tCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCt                                                      <  1:1099362/49‑1 (MQ=255)

                                       tCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCt                                                      <  1:3529766/49‑1 (MQ=255)

                                         aTTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTa                                                                  <  1:2795492/35‑1 (MQ=255)

                                         aTTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCa                                                               >  1:1027721/1‑38 (MQ=255)

                                         aTTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGAttttt                               <  1:1430234/70‑1 (MQ=255)

                                         aTTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTg                              <  1:514724/71‑1 (MQ=255)

                                         aTTCCAGCTTAGCGACATCCTGGCCGTTATGCGTATCAGCACCCCCTGGTATTGCAGCATGGTGATTTTTg                              >  1:3679760/1‑71 (MQ=255)

                                          ttCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTgcag                                            <  1:1831520/56‑1 (MQ=255)

                                          ttCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTCGTATTgcag                                            <  1:604498/56‑1 (MQ=255)

                                          ttCCAGCTTAGCGACATCCTGGCCGTTTGGCGTATCAGCACCACCTGGTATTgcagc                                           <  1:3933160/57‑1 (MQ=255)

                                          ttCCAGCTTAGCGACATCCTGGCCGTTTGGCGTATCAGCACCACCTGGTATTgcagc                                           <  1:1699367/57‑1 (MQ=255)

                                           tCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGc                            >  1:872450/1‑71 (MQ=255)

                                           tCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGc                            >  1:1814849/1‑71 (MQ=255)

                                            ccAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTa                                                  >  1:2552077/1‑48 (MQ=255)

                                            ccAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGAtt                                  >  1:3839869/1‑64 (MQ=255)

                                            ccAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCg                           >  1:2875435/1‑71 (MQ=255)

                                            ccAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCg                           >  1:3310363/1‑71 (MQ=255)

                                            ccAGCTTAGCGACATCCTGGCCGTTTTGAGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCg                           >  1:3390423/1‑71 (MQ=255)

                                               gCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCt                        <  1:1464622/71‑1 (MQ=255)

                                                      gACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCt                        <  1:3414979/64‑1 (MQ=255)

                                                         aTCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTg                                               >  1:2153850/1‑38 (MQ=255)

                                                         aTCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTg                                               >  1:768624/1‑38 (MQ=255)

                                                          tCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATc                <  1:548015/68‑1 (MQ=255)

                                                          tCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTAc             <  1:3764442/71‑1 (MQ=255)

                                                           ccTGGCCGTTTTGCGTATCAGCACCACCTGGTATTgcat                                            <  1:3191022/39‑2 (MQ=255)

                                                           ccTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACg            >  1:366509/1‑71 (MQ=255)

                                                            cTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGc           <  1:2194499/71‑1 (MQ=255)

                                                            cTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGc           >  1:2017517/1‑71 (MQ=255)

                                                            cTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGc           <  1:3287585/71‑1 (MQ=255)

                                                             tGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATc                <  1:1765622/65‑1 (MQ=255)

                                                              ggCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGa                                    <  1:527643/44‑1 (MQ=255)

                                                              ggCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGa                                    <  1:3940783/44‑1 (MQ=255)

                                                              ggCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGa                                    <  1:1681128/44‑1 (MQ=255)

                                                              ggCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCa         >  1:1932890/1‑71 (MQ=255)

                                                               gCCGTTTTGCGTATCAGCACCACCTGGTATTgcagc                                           >  1:2022017/1‑36 (MQ=255)

                                                               gCCGTTTGGCGTATCAGCACGACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCAt        >  1:4054056/1‑71 (MQ=255)

                                                                  gTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCt     <  1:2641306/71‑1 (MQ=255)

                                                                   ttttGCGTATCAGCACCACCTGGTATTGCAGCATg                                        <  1:383585/35‑1 (MQ=255)

                                                                   ttttGCGTATCAGCACCACCTGGTATTGCAGCATg                                        <  1:2000633/35‑1 (MQ=255)

                                                                   ttttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTg    <  1:1249908/71‑1 (MQ=255)

                                                                   ttttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTg    <  1:3182592/71‑1 (MQ=255)

                                                                   ttttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTg    <  1:3549802/71‑1 (MQ=255)

                                                                    tttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTg    <  1:2090997/70‑1 (MQ=255)

                                                                    tttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTg    <  1:3288440/70‑1 (MQ=255)

                                                                    tttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTg    <  1:235588/70‑1 (MQ=255)

                                                                     ttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCtt                       >  1:2497898/1‑50 (MQ=255)

                                                                     ttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTGAg  >  1:2745932/1‑71 (MQ=255)

                                                                     ttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTGAg  >  1:3691928/1‑71 (MQ=255)

                                                                     ttGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTGAg  >  1:1191674/1‑71 (MQ=255)

                                                                      tGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTGAg  <  1:1848738/70‑1 (MQ=255)

AACAACAACCTGCGCAGTGCCAATAACGAAGAAAAACAGTCATTCCAGCTTAGCGACATCCTGGCCGTTTTGCGTATCAGCACCACCTGGTATTGCAGCATGGTGATTTTTGGCGTCTTCACCATCTACGCCATTCTGAG  >  minE/1870656‑1870795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`