| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,889,046 | G→A | 14.0% | P395S (CCC→TCC) | gudX ← | predicted glucarate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,889,046 | 0 | G | A | 14.0% | 133.5 / 10.6 | 50 | P395S (CCC→TCC) | gudX | predicted glucarate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (19/24); new base A (5/2); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCT > minE/1888978‑1889114 | aGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGacg < 1:3427570/71‑1 (MQ=255) gCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCa < 1:195819/71‑1 (MQ=255) aCGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAg > 1:3563529/1‑71 (MQ=255) cGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAgg < 1:1191375/71‑1 (MQ=255) cGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAgg < 1:2526415/71‑1 (MQ=255) cGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAgg < 1:1418351/71‑1 (MQ=255) cGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAgg < 1:472233/71‑1 (MQ=255) cGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAg > 1:410641/1‑70 (MQ=255) gTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAgg > 1:937939/1‑70 (MQ=255) ttATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAAc > 1:2871190/1‑71 (MQ=255) ttATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAAc > 1:1930386/1‑71 (MQ=255) taGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCAt < 1:3700487/61‑1 (MQ=255) ccTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACTGCAAtt < 1:534432/71‑1 (MQ=255) ccTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAAtt < 1:2739004/71‑1 (MQ=255) aTGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAg > 1:2877497/1‑56 (MQ=255) aTGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTc > 1:3328885/1‑71 (MQ=255) aTGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTc > 1:2574980/1‑71 (MQ=255) aTGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTc > 1:25988/1‑71 (MQ=255) aTGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAAAGGCAATTTTTc > 1:3654054/1‑71 (MQ=255) tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCg < 1:1289480/70‑1 (MQ=255) tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCg < 1:2856511/70‑1 (MQ=255) tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGt > 1:941793/1‑71 (MQ=255) tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGt < 1:4051154/71‑1 (MQ=255) tgCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGt > 1:1583559/1‑71 (MQ=255) ccTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGtt < 1:3947755/70‑1 (MQ=255) cTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGtttt > 1:2042332/1‑71 (MQ=255) ttGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTaa > 1:1967358/1‑71 (MQ=255) ttGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTa < 1:3778408/70‑1 (MQ=255) tGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAAt > 1:1739858/1‑71 (MQ=255) aCCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAAtct > 1:2920279/1‑70 (MQ=255) gTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGaa > 1:631884/1‑42 (MQ=255) gTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGc > 1:3544442/1‑71 (MQ=255) gTTCCCAGTCCAGTTCCACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTATTTAATCTCCAGc > 1:3312382/1‑71 (MQ=255) ttCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAg < 1:2163302/69‑1 (MQ=255) ttCCCAGTCCAGTTCAACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAg < 1:782395/69‑1 (MQ=255) ccagtccagtTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGTAt < 1:2932434/71‑1 (MQ=255) gtccagtTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATGTCCAGCGGAttt < 1:1959056/70‑1 (MQ=255) gtccagtTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAttt < 1:733815/70‑1 (MQ=255) tccagtTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:818796/71‑1 (MQ=255) tccagtTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTg < 1:1175752/71‑1 (MQ=255) ttCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGAt < 1:1493524/62‑1 (MQ=255) ttCCACGCCCAGACCGGACGCATCAGGAACGG‑AATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAg > 1:1341022/1‑71 (MQ=255) tCCACGCCCAGCCCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAg > 1:3797263/1‑71 (MQ=255) ccACGCCCAGACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTaa > 1:3727489/1‑48 (MQ=255) gCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGAc < 1:3254577/71‑1 (MQ=255) cccAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACa < 1:498136/71‑1 (MQ=255) cccAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACa < 1:2126548/71‑1 (MQ=255) aGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAAt > 1:846646/1‑41 (MQ=255) gACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGa > 1:203940/1‑65 (MQ=255) gACCGGACGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCg < 1:4015333/71‑1 (MQ=255) aCCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGc < 1:3599083/71‑1 (MQ=255) gggCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGccc < 1:2151370/70‑1 (MQ=255) gggCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCcct < 1:1496114/71‑1 (MQ=255) | AGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTTAATCTCCAGCGGATTTTGGGTCAGGCGACAATCGCCCT > minE/1888978‑1889114 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |