| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,919,248 | G→A | 7.4% | Q205Q (CAG→CAA) | tas → | predicted oxidoreductase, NADP(H)‑dependent aldo‑keto reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,919,248 | 0 | G | A | 7.4% | 259.1 / 7.9 | 81 | Q205Q (CAG→CAA) | tas | predicted oxidoreductase, NADP(H)‑dependent aldo‑keto reductase |
| Reads supporting (aligned to +/- strand): ref base G (40/35); new base A (4/2); total (44/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
CTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGT > minE/1919178‑1919316 | cTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAg < 1:2803764/71‑1 (MQ=255) tGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAg > 1:1274206/1‑70 (MQ=255) tGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCt > 1:1192451/1‑70 (MQ=255) tGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCt > 1:348601/1‑70 (MQ=255) cGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACa < 1:1464185/71‑1 (MQ=255) aTGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCt > 1:2189466/1‑71 (MQ=255) aTGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCt > 1:2190759/1‑71 (MQ=255) aTGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCt > 1:1352023/1‑71 (MQ=255) aTGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCt > 1:2945940/1‑71 (MQ=255) gcgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACa > 1:1681486/1‑65 (MQ=255) gcgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACa > 1:672148/1‑65 (MQ=255) cgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGt > 1:2165623/1‑70 (MQ=255) cgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGt > 1:933319/1‑70 (MQ=255) cgcTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGt > 1:1075667/1‑70 (MQ=255) cTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTaa < 1:1148983/71‑1 (MQ=255) tGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCt > 1:2251939/1‑61 (MQ=255) tGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTaaa < 1:2313461/67‑1 (MQ=255) tGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAAc > 1:599156/1‑68 (MQ=255) gCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCg > 1:268449/1‑69 (MQ=255) gCATCTGGCGGACAAACACGATCTGACGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCg > 1:2361501/1‑69 (MQ=255) tCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTa < 1:505989/61‑1 (MQ=255) tGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGtt > 1:2790697/1‑69 (MQ=255) ggCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAg < 1:3054044/66‑1 (MQ=255) gCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAAc > 1:3422590/1‑60 (MQ=255) gCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTg < 1:2515201/70‑1 (MQ=255) gCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTg < 1:205389/70‑1 (MQ=255) cGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa < 1:774165/71‑1 (MQ=255) ggACAAACACGATCTGCCGCGTATTGTCACCATTCAGAAc < 1:960069/40‑1 (MQ=255) gACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTg < 1:2060279/67‑1 (MQ=255) gACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGt < 1:4093384/71‑1 (MQ=255) cAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGttt > 1:4116732/1‑63 (MQ=255) cAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGt < 1:273396/61‑1 (MQ=255) aaaCACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAgg < 1:3675146/71‑1 (MQ=255) aaCACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTAc < 1:1693907/41‑1 (MQ=255) aaCACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTAc < 1:856205/41‑1 (MQ=255) acacGATCTGCCGCGTATTGTCACCATTCAGAACCCtt < 1:1400396/38‑1 (MQ=255) cacGATCTGCCGCGTATTGTCACCATTCAGAACCCTTa > 1:1176094/1‑38 (MQ=255) cacGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGtt > 1:2519426/1‑47 (MQ=255) cacGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTaaa > 1:720925/1‑50 (MQ=255) cacGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTaaa > 1:159509/1‑50 (MQ=255) cacGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTaaa > 1:1667785/1‑50 (MQ=255) cacGATCTGCCGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCt > 1:1674363/1‑71 (MQ=255) aTCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGtttt < 1:231049/56‑1 (MQ=255) aTCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGt < 1:941752/53‑1 (MQ=255) cTGCCGCGTATTGTCACCATTCAGAACCCTTACAg > 1:3520672/1‑35 (MQ=255) ccGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAg < 1:1795153/67‑1 (MQ=255) ccGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAg < 1:2965657/67‑1 (MQ=255) ccGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAg < 1:3957901/70‑1 (MQ=255) ccGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGt > 1:2117798/1‑71 (MQ=255) ccGCGTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAg < 1:262363/67‑1 (MQ=255) cgcgTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAg > 1:3382998/1‑54 (MQ=255) gcgTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCa < 1:1456395/71‑1 (MQ=255) gcgTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTa > 1:1860371/1‑55 (MQ=255) gcgTATTGTCACCATTCAAAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTa > 1:3027881/1‑55 (MQ=255) gTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGt < 1:367577/52‑1 (MQ=255) gTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGc < 1:2843355/71‑1 (MQ=255) tATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGc > 1:2056869/1‑70 (MQ=255) ttGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGt > 1:2656004/1‑64 (MQ=255) ttGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCa > 1:1177571/1‑70 (MQ=255) ttGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAg < 1:2491931/71‑1 (MQ=255) tGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAg > 1:3586519/1‑70 (MQ=255) gTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAg > 1:2583454/1‑49 (MQ=255) gTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTa > 1:1603447/1‑71 (MQ=255) tCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGaa > 1:2226197/1‑44 (MQ=255) tCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGa < 1:651859/43‑1 (MQ=255) tCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTAt > 1:3093933/1‑71 (MQ=255) aCCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAg < 1:3568470/55‑1 (MQ=255) aCCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATg < 1:4114133/70‑1 (MQ=255) ccATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGt < 1:3429933/47‑1 (MQ=255) ccATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGt < 1:545102/47‑1 (MQ=255) ccATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATg > 1:544132/1‑69 (MQ=255) ccATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGaa < 1:1225654/71‑1 (MQ=255) ccATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGACAGCCAGTATGa < 1:3983659/70‑1 (MQ=255) ccATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTAGAAGTAGGTCTGGCAGAAGTCAGCCAGTATg > 1:589816/1‑69 (MQ=255) cATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCt < 1:1562552/48‑1 (MQ=255) aTTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAgg > 1:1984431/1‑71 (MQ=255) ttCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTg < 1:868389/36‑1 (MQ=255) ttCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAg > 1:756432/1‑39 (MQ=255) ttCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAg < 1:1031413/51‑1 (MQ=255) ttCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAggg < 1:3315114/71‑1 (MQ=255) tCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGc > 1:3911131/1‑58 (MQ=255) cAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATg > 1:3666834/1‑64 (MQ=255) cAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGt > 1:2369806/1‑71 (MQ=255) | CTGCATTTGGCGTAATGCGCTACCTGCATCTGGCGGACAAACACGATCTGCCGCGTATTGTCACCATTCAGAACCCTTACAGTCTGTTAAACCGCAGTTTTGAAGTAGGTCTGGCAGAAGTCAGCCAGTATGAAGGGGT > minE/1919178‑1919316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |