| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,924,650 | C→T | 30.0% | H339Y (CAT→TAT) | galR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,924,650 | 0 | C | T | 30.0% | 90.0 / 44.7 | 60 | H339Y (CAT→TAT) | galR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (26/16); new base T (6/12); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGC > minE/1924581‑1924714 | aCTAATGTCTTTAGTCCCACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcatca > 1:2645540/1‑71 (MQ=255) aaTGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcatca > 1:2317958/1‑68 (MQ=255) tCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAAc > 1:3501747/1‑70 (MQ=255) cTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAAc > 1:992267/1‑69 (MQ=255) tAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTcatcat > 1:92828/1‑61 (MQ=255) tAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGc < 1:2812026/70‑1 (MQ=255) tAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGc < 1:334661/70‑1 (MQ=255) cGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTaa > 1:583103/1‑71 (MQ=255) cGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTa < 1:3403271/70‑1 (MQ=255) aCGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAAcc > 1:1072089/1‑71 (MQ=255) gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCg > 1:1300586/1‑70 (MQ=255) gCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCg > 1:2598927/1‑70 (MQ=255) cTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCg > 1:3805644/1‑69 (MQ=255) ggTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGa < 1:2779318/60‑1 (MQ=255) ggTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGt > 1:3944099/1‑71 (MQ=255) ggTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGt > 1:2723467/1‑71 (MQ=255) gTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGt > 1:3740671/1‑70 (MQ=255) gTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGt > 1:265050/1‑70 (MQ=255) aCGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTa > 1:2382274/1‑70 (MQ=255) tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGc < 1:1521330/71‑1 (MQ=255) tcgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGc < 1:3104918/71‑1 (MQ=255) cgtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGc < 1:2123388/70‑1 (MQ=255) gtcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCaa > 1:703120/1‑71 (MQ=255) tcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGc < 1:1531850/49‑1 (MQ=255) tcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGc < 1:1536798/49‑1 (MQ=255) tcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCa > 1:3928687/1‑47 (MQ=255) tcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAAt < 1:1793073/71‑1 (MQ=255) tcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAAt < 1:860886/71‑1 (MQ=255) tcATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAAt < 1:2785948/71‑1 (MQ=255) cATTCAGTGTCAACTCCGTCGCTGGAGGCAAGtcatca > 1:411289/1‑38 (MQ=255) cATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTaa > 1:3624435/1‑54 (MQ=255) aTTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAAtt < 1:1536827/70‑1 (MQ=255) ttCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCa < 1:2057074/40‑1 (MQ=255) ttCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCa < 1:3173563/40‑1 (MQ=255) ttCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAAtt < 1:474253/69‑1 (MQ=255) tCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGc < 1:1988416/64‑1 (MQ=255) cAGTGTCAACTCCGTCGCTGGAGGCAAGTCATTATGCAAcc > 1:3485428/1‑41 (MQ=255) tgtCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAAt > 1:2796504/1‑63 (MQ=255) tCAACTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAgcgc > 1:1467553/1‑69 (MQ=255) aaCTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGt < 1:899326/71‑1 (MQ=255) aaCTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAg > 1:1608053/1‑70 (MQ=255) aaCTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAg > 1:2428639/1‑70 (MQ=255) cTCCGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTaa > 1:3880113/1‑71 (MQ=255) cTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTaa > 1:331217/1‑71 (MQ=255) ccGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAtt > 1:3160358/1‑71 (MQ=255) ccGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAt > 1:3541615/1‑70 (MQ=255) ccGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAt > 1:505473/1‑70 (MQ=255) cGTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttc > 1:2480538/1‑71 (MQ=255) cGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAtt < 1:1451094/70‑1 (MQ=255) gTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttct < 1:114077/71‑1 (MQ=255) gTCGCTGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttct < 1:1050289/71‑1 (MQ=255) tCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTaa > 1:3840658/1‑66 (MQ=255) gCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttcttc > 1:1466364/1‑70 (MQ=255) gCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttcttc < 1:604473/70‑1 (MQ=255) gCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttcttc < 1:3505627/70‑1 (MQ=255) tGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGc < 1:522733/45‑1 (MQ=255) tGGAGGCAAGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGc < 1:3709634/45‑1 (MQ=255) tGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGAt > 1:2472574/1‑71 (MQ=255) tGGAGGCAAGTAATTATGCAACCAGCGACTAACCGCAGTTAAAGc < 1:695887/45‑1 (MQ=255) gAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATgg > 1:2215776/1‑71 (MQ=255) aGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGt < 1:2967739/71‑1 (MQ=255) aaGTCATTATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTgg > 1:1164843/1‑71 (MQ=255) aGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGc < 1:909052/71‑1 (MQ=255) gTCATCATGAAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGc > 1:31445/1‑70 (MQ=255) tcatcaTGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAAttcttc < 1:1760563/58‑1 (MQ=255) catcatGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGa > 1:271852/1‑59 (MQ=255) catGCAACCAGCGACTAACCGCAGTTAAAGCAATTc > 1:2258137/1‑36 (MQ=255) | ACTAATGTCTTTAGTCCGACGCTGGTACGTCGTCATTCAGTGTCAACTCCGTCGCTGGAGGCAAGTCATCATGCAACCAGCGACTAACCGCAGTTAAAGCAATTCCAGCGCCAGTAATTCTTCGATGGTCTGGC > minE/1924581‑1924714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |