| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,932,763 | G→A | 23.3% | intergenic (‑134/+162) | yqeF ← / ← lysS | predicted acyltransferase/lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,932,763 | 0 | G | A | 23.3% | 143.4 / 41.3 | 73 | intergenic (‑134/+162) | yqeF/lysS | predicted acyltransferase/lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (36/20); new base A (9/8); total (45/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01 | |||||||||||
AAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTT > minE/1932696‑1932831 | aaaaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAagg < 1:3439567/70‑1 (MQ=255) aaaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGt < 1:1569027/70‑1 (MQ=255) aaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTg > 1:1491432/1‑70 (MQ=255) aaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGc > 1:1402998/1‑70 (MQ=255) aaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGc < 1:2507680/70‑1 (MQ=255) aaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGc > 1:4058779/1‑70 (MQ=255) aGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCaa > 1:2180925/1‑71 (MQ=255) aGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCaa > 1:3930384/1‑71 (MQ=255) gTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAAc < 1:1868208/71‑1 (MQ=255) gTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAAc < 1:3497069/71‑1 (MQ=255) tGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCTCGTAAGGTTTGc < 1:2502713/67‑1 (MQ=255) aTTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCAc > 1:1187111/1‑71 (MQ=255) aTTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCAc > 1:1247301/1‑71 (MQ=255) tttttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCAc > 1:3490667/1‑70 (MQ=255) tttttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACt < 1:3431402/71‑1 (MQ=255) ttttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACt > 1:701451/1‑70 (MQ=255) tttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTaa > 1:2346302/1‑71 (MQ=255) tttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGCTTGCAACCa > 1:1529625/1‑67 (MQ=255) tATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAAcc > 1:3492734/1‑71 (MQ=255) cacaAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACccac > 1:1076358/1‑71 (MQ=255) cacaAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACccac > 1:2478804/1‑71 (MQ=255) caAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACccacc > 1:1124206/1‑70 (MQ=255) ggAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa < 1:2039530/70‑1 (MQ=255) gAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCa < 1:2540283/51‑1 (MQ=255) aaaTATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATagag > 1:3496655/1‑71 (MQ=255) atGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATagag > 1:1218107/1‑67 (MQ=255) atGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATa > 1:664460/1‑64 (MQ=255) tGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAgggg > 1:2335473/1‑69 (MQ=255) tGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa > 1:621268/1‑63 (MQ=255) ccTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGa < 1:2120129/71‑1 (MQ=255) ccTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGa < 1:3130629/71‑1 (MQ=255) gAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAg > 1:3407217/1‑71 (MQ=255) agcagcAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATaga > 1:3286366/1‑59 (MQ=255) agcagcAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAgg > 1:1065714/1‑71 (MQ=255) gcagcaGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATa > 1:1612672/1‑56 (MQ=255) gcagTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGt > 1:2584162/1‑71 (MQ=255) agTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGtt > 1:453354/1‑70 (MQ=255) agTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGtt > 1:3455838/1‑70 (MQ=255) agTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTa < 1:2605248/71‑1 (MQ=255) agTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTa < 1:4141996/71‑1 (MQ=255) gTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTaa > 1:1256509/1‑71 (MQ=255) gTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTaa > 1:2251381/1‑71 (MQ=255) gTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTa < 1:571022/39‑1 (MQ=255) tCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACccacc < 1:3577262/45‑1 (MQ=255) tCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTa < 1:3957540/57‑1 (MQ=255) tCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTa < 1:2795991/57‑1 (MQ=255) cAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATaga > 1:588979/1‑50 (MQ=255) agagACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGa > 1:3366054/1‑57 (MQ=255) agagACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGa > 1:3375888/1‑57 (MQ=255) agagACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAAtct > 1:1787905/1‑71 (MQ=255) agagACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATTGAGGGGTAGATAGGGCGTTAAtct < 1:1151723/71‑1 (MQ=255) agagACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAAtct > 1:1094294/1‑71 (MQ=255) agaCATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTcc < 1:3003287/71‑1 (MQ=255) aCATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCaa < 1:1020090/41‑1 (MQ=255) aCATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGtt < 1:2639690/62‑1 (MQ=255) aCATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa > 1:1394042/1‑43 (MQ=255) cATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTa > 1:3934314/1‑50 (MQ=255) cATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCAt < 1:3430958/71‑1 (MQ=255) cATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCAt < 1:3799753/71‑1 (MQ=255) cATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCAt < 1:4142878/71‑1 (MQ=255) aTAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATa < 1:3441641/41‑1 (MQ=255) aCTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAggg > 1:3024881/1‑43 (MQ=255) aCTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACt > 1:3861796/1‑70 (MQ=255) aCTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACtt < 1:3692851/71‑1 (MQ=255) tGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTaa > 1:3978271/1‑71 (MQ=255) gCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAAc > 1:870890/1‑70 (MQ=255) cACGTAGGGTCTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCt > 1:2673599/1‑71 (MQ=255) gTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTa < 1:3724379/50‑1 (MQ=255) gTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATa < 1:3349752/60‑1 (MQ=255) gTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGt > 1:3473111/1‑71 (MQ=255) gTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGt > 1:3217499/1‑71 (MQ=255) tAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGtt < 1:3354384/71‑1 (MQ=255) aGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGt > 1:3631091/1‑46 (MQ=255) aGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTcc > 1:857645/1‑54 (MQ=255) | AAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTT > minE/1932696‑1932831 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |