Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,030 C→T 6.8% P471P (CCG→CCA lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,0300CT 6.8% 286.4 / 7.6 88P471P (CCG→CCAlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (43/39);  new base T (4/2);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACC  >  minE/1932963‑1933095
                                                                   |                                                                 
cgcgGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggc                                                                >  1:221498/1‑71 (MQ=255)
 gcgGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATGCCAATTCCCAGACCTGCTGTcggcg                                                               >  1:3436208/1‑71 (MQ=255)
 gcgGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcg                                                               >  1:3368033/1‑71 (MQ=255)
 gcgGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcg                                                               >  1:2142807/1‑71 (MQ=255)
   ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg                                                              <  1:4001123/70‑1 (MQ=255)
       ggTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc                                                         >  1:867524/1‑71 (MQ=255)
        gTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGcc                                                        <  1:3749108/71‑1 (MQ=255)
        gTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGcc                                                        <  1:241087/71‑1 (MQ=255)
        gTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc                                                         <  1:1585912/70‑1 (MQ=255)
          aTGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCa                                                       >  1:3369541/1‑70 (MQ=255)
           tGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt                                                      <  1:3818248/70‑1 (MQ=255)
           tGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt                                                      <  1:88763/70‑1 (MQ=255)
             gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg                                                                 >  1:697740/1‑57 (MQ=255)
             gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGtt                                                   >  1:3394044/1‑71 (MQ=255)
               tGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCa                                                       >  1:2192936/1‑65 (MQ=255)
               tGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc                                                  >  1:2915820/1‑70 (MQ=255)
                     cgAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg                                           <  1:4064093/70‑1 (MQ=255)
                      gAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg                                           >  1:2354297/1‑70 (MQ=255)
                       aaCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc                                                         <  1:1171582/55‑1 (MQ=255)
                       aaCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCa                                                       >  1:2306394/1‑57 (MQ=255)
                       aaCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt                                         >  1:2655096/1‑71 (MQ=255)
                       aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCATACCATATTCCAgcgcag                                          >  1:2150261/1‑68 (MQ=255)
                       aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGt                                         >  1:3427411/1‑71 (MQ=255)
                       aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGt                                         >  1:127449/1‑71 (MQ=255)
                         cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGa                                       >  1:3129245/1‑71 (MQ=255)
                         cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGa                                       >  1:3147988/1‑71 (MQ=255)
                          aGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGAc                                      >  1:1200501/1‑71 (MQ=255)
                            cATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACg                                     >  1:770972/1‑70 (MQ=255)
                              ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTcg                                                                  <  1:3235062/39‑1 (MQ=255)
                              ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGt                                    >  1:684961/1‑69 (MQ=255)
                              ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa                                  <  1:1201579/71‑1 (MQ=255)
                              ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa                                  <  1:2139306/71‑1 (MQ=255)
                              ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa                                  >  1:4100059/1‑71 (MQ=255)
                                aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc                                >  1:1759097/1‑71 (MQ=255)
                                aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc                                >  1:153411/1‑71 (MQ=255)
                                 ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATg                                                     >  1:2943435/1‑49 (MQ=255)
                                 ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATg                                                     >  1:1646375/1‑49 (MQ=255)
                                 ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCt                               >  1:174225/1‑71 (MQ=255)
                                  cATACGGTCGATACCAATTCCCAGACCTGCTGTcggcg                                                               >  1:1366851/1‑38 (MQ=255)
                                  cATACGGTCGATACCAATTCCCAGACCTGCTGTcggcg                                                               >  1:856634/1‑38 (MQ=255)
                                  cATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt                              <  1:96404/71‑1 (MQ=255)
                                   attcGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTc                             >  1:3705486/4‑71 (MQ=255)
                                   aTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc                                <  1:727059/68‑1 (MQ=255)
                                   aTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTc                             <  1:1332598/71‑1 (MQ=255)
                                    tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAg                                                          <  1:4060666/41‑1 (MQ=255)
                                    tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATg                                                     <  1:2368491/46‑1 (MQ=255)
                                     aCGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGc                                            <  1:811034/54‑1 (MQ=255)
                                      cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt                           <  1:3354042/70‑1 (MQ=255)
                                      cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATc                          >  1:2674786/1‑71 (MQ=255)
                                      cGGTCGATACCAATTCCCAGACCTGCTGTCGGCAGTAAGCCATGTTCCACTGCGGTGACGTAATCTTCATc                          >  1:997318/1‑71 (MQ=255)
                                           gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg                      <  1:3042518/70‑1 (MQ=255)
                                            aTAGCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGaa                    <  1:2580252/71‑1 (MQ=255)
                                            aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa                    <  1:2808897/71‑1 (MQ=255)
                                            aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa                    <  1:2234781/71‑1 (MQ=255)
                                              aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt                                         >  1:1462464/1‑48 (MQ=255)
                                              aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa                  >  1:1505129/1‑71 (MQ=255)
                                               ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa                  <  1:3797704/70‑1 (MQ=255)
                                               ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa                  >  1:546528/1‑70 (MQ=255)
                                               ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACAt                 <  1:4037924/71‑1 (MQ=255)
                                               ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACAt                 <  1:377206/71‑1 (MQ=255)
                                               ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACAt                 <  1:3956211/71‑1 (MQ=255)
                                                  aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa                  >  1:2628690/1‑67 (MQ=255)
                                                  aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa                  >  1:2290176/1‑67 (MQ=255)
                                                   ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg                         <  1:1210598/59‑1 (MQ=255)
                                                   ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc                <  1:436537/68‑1 (MQ=255)
                                                   ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt             >  1:358568/1‑71 (MQ=255)
                                                    tCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt                              >  1:2958831/1‑53 (MQ=255)
                                                    tCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCtt            >  1:1759730/1‑71 (MQ=255)
                                                     cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg                                           >  1:2142245/1‑39 (MQ=255)
                                                     cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGAc                                      >  1:2129808/1‑44 (MQ=255)
                                                     cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc           >  1:2614259/1‑71 (MQ=255)
                                                      ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc           <  1:3279410/70‑1 (MQ=255)
                                                      ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc           <  1:3062278/70‑1 (MQ=255)
                                                       cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa                                   <  1:3883741/45‑1 (MQ=255)
                                                       cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa                                   <  1:1052931/45‑1 (MQ=255)
                                                       cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa                                   <  1:2728139/45‑1 (MQ=255)
                                                       cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt         >  1:3439755/1‑71 (MQ=255)
                                                       cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt         >  1:2620179/1‑71 (MQ=255)
                                                        aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg                      <  1:1768908/57‑1 (MQ=255)
                                                        aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc        <  1:1029968/71‑1 (MQ=255)
                                                        aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc        <  1:3570969/71‑1 (MQ=255)
                                                          aCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGt                                    <  1:2571890/41‑1 (MQ=255)
                                                          aCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt      <  1:2796468/71‑1 (MQ=255)
                                                           ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtcgg      >  1:2149095/1‑69 (MQ=255)
                                                           ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt      >  1:1793098/1‑70 (MQ=255)
                                                           ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc     <  1:2487568/71‑1 (MQ=255)
                                                            ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc                                >  1:70646/1‑43 (MQ=255)
                                                            ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc     <  1:109452/70‑1 (MQ=255)
                                                              gctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAcc  >  1:2749653/1‑71 (MQ=255)
                                                              gctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAcc  >  1:3228973/1‑71 (MQ=255)
                                                               ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg                      >  1:487836/1‑50 (MQ=255)
                                                               ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGaa                    >  1:2553085/1‑52 (MQ=255)
                                                               ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc   >  1:2167631/1‑69 (MQ=255)
                                                                  tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa                  >  1:925193/1‑51 (MQ=255)
                                                                  tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt             <  1:224303/56‑1 (MQ=255)
                                                                  tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt             <  1:1998831/56‑1 (MQ=255)
                                                                  tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt             <  1:1387151/56‑1 (MQ=255)
                                                                   |                                                                 
CGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACC  >  minE/1932963‑1933095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: