| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 242,863 | G→A | 6.6% | Q454* (CAA→TAA) | sbcC ← | exonuclease, dsDNA, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 242,863 | 0 | G | A | 6.6% | 296.5 / 7.5 | 91 | Q454* (CAA→TAA) | sbcC | exonuclease, dsDNA, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (47/38); new base A (3/3); total (50/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 | |||||||||||
AGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGC > minE/242793‑242933 | aGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTg < 1:3140184/71‑1 (MQ=255) gTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGc > 1:224984/1‑71 (MQ=255) gctgcGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGt < 1:895784/70‑1 (MQ=255) gctgcGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGt < 1:3035096/70‑1 (MQ=255) ctgcGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTg < 1:2171390/70‑1 (MQ=255) cGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACa < 1:3832308/70‑1 (MQ=255) cGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACa < 1:793247/70‑1 (MQ=255) cGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACa < 1:3142750/70‑1 (MQ=255) cGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACAt < 1:3085542/71‑1 (MQ=255) gTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACAtt < 1:2729276/71‑1 (MQ=255) tcttttctttATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACAtt < 1:441588/70‑1 (MQ=255) cttttctttATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTACGTGa < 1:1901237/65‑1 (MQ=255) ttttctttATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTc > 1:2984996/1‑69 (MQ=255) ttttctttATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTg > 1:3568630/1‑71 (MQ=255) ttttctttATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTg > 1:3009743/1‑71 (MQ=255) tttctttATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGAc > 1:3161957/1‑64 (MQ=255) tttctttATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGAc > 1:3431698/1‑64 (MQ=255) ttctttATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACAtt > 1:636827/1‑66 (MQ=255) ttctttATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCt > 1:3253692/1‑68 (MQ=255) tataACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGc < 1:677254/70‑1 (MQ=255) tataACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTACGTGa > 1:2286238/1‑57 (MQ=255) taACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACa < 1:1511812/57‑1 (MQ=255) taACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCg < 1:3169097/69‑1 (MQ=255) taACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGAc < 1:2085506/71‑1 (MQ=255) aCGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGa > 1:3540013/1‑53 (MQ=255) gCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGAc > 1:645123/1‑67 (MQ=255) gCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGt < 1:781530/71‑1 (MQ=255) gCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGt < 1:1577584/71‑1 (MQ=255) cTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTg > 1:3068664/1‑45 (MQ=255) cTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTg > 1:1345122/1‑45 (MQ=255) ggCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTg < 1:2938473/55‑1 (MQ=255) gcgcATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAAc < 1:1687072/70‑1 (MQ=255) cgcATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTg > 1:4042846/1‑53 (MQ=255) cgcATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTg > 1:2777830/1‑53 (MQ=255) cATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAAc > 1:153548/1‑67 (MQ=255) cATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTgcg > 1:3718571/1‑71 (MQ=255) aTTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGa < 1:2134876/43‑1 (MQ=255) tttCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGt < 1:3527934/40‑1 (MQ=255) tttCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGt < 1:914090/40‑1 (MQ=255) tttCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACa < 1:2905507/44‑1 (MQ=255) tCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTg < 1:3659018/47‑1 (MQ=255) tCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGa < 1:1084939/55‑1 (MQ=255) tCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTgcgc > 1:2722128/1‑68 (MQ=255) tCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTgcgc > 1:3350938/1‑68 (MQ=255) tCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTgcgc > 1:2945153/1‑68 (MQ=255) cGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGa > 1:2335351/1‑48 (MQ=255) aaGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGAGATTCTGGa > 1:542564/1‑44 (MQ=255) aaGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACa > 1:376879/1‑37 (MQ=255) aaGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGa > 1:1350999/1‑44 (MQ=255) aaGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGtt > 1:2173527/1‑71 (MQ=255) tGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGa > 1:2586987/1‑41 (MQ=255) gcggcgTTACGTTGCGTCTGTTTTTGCGTGACATTCTGGATAGc > 1:3488705/1‑44 (MQ=255) gcggcgTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGAt > 1:679556/1‑41 (MQ=255) gcggcgTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGAc > 1:1483328/1‑47 (MQ=255) gcggcgTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTaa > 1:2355750/1‑53 (MQ=255) ggcgTTACGTTGCGTCTGTTCTTGCGTGACATTCTg < 1:1754292/36‑1 (MQ=255) ttACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGttttt < 1:1383856/64‑1 (MQ=255) ttACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTttgt < 1:780055/66‑1 (MQ=255) ttACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTggg > 1:1608224/1‑70 (MQ=255) aCGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGAc > 1:3641982/1‑39 (MQ=255) aCGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAAc > 1:1855310/1‑46 (MQ=255) aCGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTttgttg > 1:2924546/1‑66 (MQ=255) aCGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGaa > 1:3113624/1‑71 (MQ=255) aCGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGaa > 1:2515588/1‑71 (MQ=255) aCGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGaa > 1:1120377/1‑71 (MQ=255) aCGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGaa > 1:2346815/1‑71 (MQ=255) aCGTTGCGTCTGTTCTTACGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGaa > 1:855321/1‑71 (MQ=255) cGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGaa > 1:1527150/1‑70 (MQ=255) gTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTTCGCCAGACGTTTTTGTTGGGGAACa < 1:1642179/71‑1 (MQ=255) gTTGCGTCTGTTCTTACGTGACATTCTGGATAGCGACCTGt < 1:2603512/41‑1 (MQ=255) ttGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGt < 1:2897774/40‑1 (MQ=255) ttGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGtt < 1:1809274/56‑1 (MQ=255) ttGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGtt < 1:1457609/56‑1 (MQ=255) ttGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACaa > 1:3994234/1‑71 (MQ=255) ttGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACaa < 1:1056354/71‑1 (MQ=255) gCGTCTGTTCTTGCGTGACATTCTGTATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAAt < 1:2886894/70‑1 (MQ=255) gCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTttgt > 1:2850586/1‑59 (MQ=255) gCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAAt < 1:1653859/70‑1 (MQ=255) gTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTg > 1:2281243/1‑71 (MQ=255) tCTGTTCTTACGTGACATTCTGGATACCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGaa < 1:1664879/63‑1 (MQ=255) tGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTc > 1:2892480/1‑70 (MQ=255) tGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTc > 1:3525308/1‑70 (MQ=255) tGTTCTTACGTGACATTCTGGATAGCGACCTGTACCTGCGCCAGACGTTTTTGTTggg > 1:2729346/1‑58 (MQ=38) gTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTttgt > 1:1011985/1‑53 (MQ=255) ttCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACg < 1:89279/45‑1 (MQ=255) ttCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGttt > 1:2751219/1‑48 (MQ=255) ttCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAAc < 1:414230/60‑1 (MQ=255) ttCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAAc < 1:1621359/60‑1 (MQ=255) ttCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACa > 1:4074965/1‑61 (MQ=255) ttCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACa < 1:1143621/61‑1 (MQ=255) tCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGtttt > 1:1997789/1‑48 (MQ=255) tCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGtttt > 1:1662459/1‑48 (MQ=255) cTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATg > 1:2494708/1‑70 (MQ=255) cTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATg < 1:2268888/70‑1 (MQ=255) gCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGc > 1:618601/1‑68 (MQ=255) gCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCa < 1:282014/69‑1 (MQ=255) gCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCa < 1:3208877/69‑1 (MQ=255) gCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAgc > 1:1251075/1‑71 (MQ=255) | AGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTCTGGATAGCGACCTGTAACTGCGCCAGACGTTTTTGTTGGGGAACAATCTGTCCATGCAGC > minE/242793‑242933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |