| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,044 | G→A | 6.0% | H467Y (CAT→TAT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,044 | 0 | G | A | 6.0% | 331.9 / 7.3 | 100 | H467Y (CAT→TAT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (45/49); new base A (4/2); total (49/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
GCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAC > minE/1932976‑1933113 | gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGtt > 1:3394044/1‑71 (MQ=255) tGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc > 1:2915820/1‑70 (MQ=255) cgAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg < 1:4064093/70‑1 (MQ=255) gAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg > 1:2354297/1‑70 (MQ=255) aaCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt > 1:2655096/1‑71 (MQ=255) aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCATACCATATTCCAgcgcag > 1:2150261/1‑68 (MQ=255) aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGt > 1:127449/1‑71 (MQ=255) cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGa > 1:3129245/1‑71 (MQ=255) cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGa > 1:3147988/1‑71 (MQ=255) aGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGAc > 1:1200501/1‑71 (MQ=255) cATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACg > 1:770972/1‑70 (MQ=255) ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGt > 1:684961/1‑69 (MQ=255) ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa < 1:1201579/71‑1 (MQ=255) ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa < 1:2139306/71‑1 (MQ=255) ttACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa > 1:4100059/1‑71 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc > 1:153411/1‑71 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc > 1:1759097/1‑71 (MQ=255) ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATg > 1:2943435/1‑49 (MQ=255) ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATg > 1:1646375/1‑49 (MQ=255) ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCt > 1:174225/1‑71 (MQ=255) cATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt < 1:96404/71‑1 (MQ=255) attcGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTc > 1:3705486/4‑71 (MQ=255) aTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc < 1:727059/68‑1 (MQ=255) aTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTc < 1:1332598/71‑1 (MQ=255) tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATg < 1:2368491/46‑1 (MQ=255) aCGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGc < 1:811034/54‑1 (MQ=255) cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt < 1:3354042/70‑1 (MQ=255) cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATc > 1:2674786/1‑71 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg < 1:3042518/70‑1 (MQ=255) aTAGCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGaa < 1:2580252/71‑1 (MQ=255) aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa < 1:2808897/71‑1 (MQ=255) aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa < 1:2234781/71‑1 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt > 1:1462464/1‑48 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa > 1:1505129/1‑71 (MQ=255) ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa > 1:546528/1‑70 (MQ=255) ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa < 1:3797704/70‑1 (MQ=255) ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACAt < 1:3956211/71‑1 (MQ=255) ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACAt < 1:4037924/71‑1 (MQ=255) ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACAt < 1:377206/71‑1 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa > 1:2628690/1‑67 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa > 1:2290176/1‑67 (MQ=255) ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg < 1:1210598/59‑1 (MQ=255) ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc < 1:436537/68‑1 (MQ=255) ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt > 1:358568/1‑71 (MQ=255) tCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt > 1:2958831/1‑53 (MQ=255) tCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCtt > 1:1759730/1‑71 (MQ=255) cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg > 1:2142245/1‑39 (MQ=255) cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGAc > 1:2129808/1‑44 (MQ=255) cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc > 1:2614259/1‑71 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:3279410/70‑1 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:3062278/70‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa < 1:1052931/45‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa < 1:2728139/45‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa < 1:3883741/45‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:2620179/1‑71 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:3439755/1‑71 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg < 1:1768908/57‑1 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc < 1:1029968/71‑1 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc < 1:3570969/71‑1 (MQ=255) aCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGt < 1:2571890/41‑1 (MQ=255) aCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:2796468/71‑1 (MQ=255) ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtcgg > 1:2149095/1‑69 (MQ=255) ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt > 1:1793098/1‑70 (MQ=255) ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc < 1:2487568/71‑1 (MQ=255) ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc > 1:70646/1‑43 (MQ=255) ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc < 1:109452/70‑1 (MQ=255) gctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAcc > 1:2749653/1‑71 (MQ=255) gctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAcc > 1:3228973/1‑71 (MQ=255) ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:487836/1‑50 (MQ=255) ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGaa > 1:2553085/1‑52 (MQ=255) tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa > 1:925193/1‑51 (MQ=255) tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt < 1:1387151/56‑1 (MQ=255) tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt < 1:1998831/56‑1 (MQ=255) tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt < 1:224303/56‑1 (MQ=255) ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTc > 1:3454360/1‑71 (MQ=255) ggcggTAAGCCATGTTACAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTc > 1:3760169/1‑71 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg < 1:3008451/41‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc < 1:1312081/47‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCg < 1:3195856/65‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCg < 1:1282271/65‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc < 1:516229/46‑1 (MQ=255) aaGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACAt > 1:3995179/1‑44 (MQ=255) aaGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgc > 1:3583245/1‑71 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt > 1:2092251/1‑54 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt > 1:371267/1‑54 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg > 1:311101/1‑71 (MQ=255) ccATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc < 1:2123592/71‑1 (MQ=255) cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:996445/46‑1 (MQ=255) cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCa < 1:3625449/71‑1 (MQ=255) cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCa < 1:152277/71‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg < 1:1349057/64‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg < 1:2522804/64‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg < 1:994455/64‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCaa < 1:1067070/70‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCa < 1:1929935/69‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc < 1:363002/71‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc < 1:1771479/71‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc > 1:1174749/1‑71 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc < 1:3492382/71‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc < 1:3954621/71‑1 (MQ=255) | GCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAC > minE/1932976‑1933113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |