| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,081 | C→G | 8.2% | A454A (GCG→GCC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,081 | 0 | C | G | 8.2% | 289.6 / 11.2 | 97 | A454A (GCG→GCC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (48/41); new base G (3/5); total (51/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
TTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGT > minE/1933014‑1933148 | ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc < 1:436537/68‑1 (MQ=255)ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt > 1:358568/1‑71 (MQ=255) tCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCtt > 1:1759730/1‑71 (MQ=255) cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc > 1:2614259/1‑71 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:3062278/70‑1 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:3279410/70‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:3439755/1‑71 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:2620179/1‑71 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc < 1:1029968/71‑1 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc < 1:3570969/71‑1 (MQ=255) aCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:2796468/71‑1 (MQ=255) ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtcgg > 1:2149095/1‑69 (MQ=255) ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt > 1:1793098/1‑70 (MQ=255) ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc < 1:2487568/71‑1 (MQ=255) ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc < 1:109452/70‑1 (MQ=255) gctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAcc > 1:2749653/1‑71 (MQ=255) gctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAcc > 1:3228973/1‑71 (MQ=255) ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc > 1:2167631/1‑69 (MQ=255) tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt < 1:1387151/56‑1 (MQ=255) tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt < 1:224303/56‑1 (MQ=255) tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCt < 1:1998831/56‑1 (MQ=255) ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTc > 1:3454360/1‑71 (MQ=255) ggcggTAAGCCATGTTACAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTc > 1:3760169/1‑71 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc < 1:1312081/47‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCg < 1:3195856/65‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCg < 1:1282271/65‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc < 1:516229/46‑1 (MQ=255) aaGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgc > 1:3583245/1‑71 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt > 1:371267/1‑54 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt > 1:2092251/1‑54 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg > 1:311101/1‑71 (MQ=255) ccATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc < 1:2123592/71‑1 (MQ=255) cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:996445/46‑1 (MQ=255) cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCa < 1:3625449/71‑1 (MQ=255) cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCa < 1:152277/71‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg < 1:994455/64‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg < 1:1349057/64‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg < 1:2522804/64‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCaa < 1:1067070/70‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCa < 1:1929935/69‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc < 1:3954621/71‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc < 1:363002/71‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc < 1:1771479/71‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc > 1:1174749/1‑71 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc < 1:3492382/71‑1 (MQ=255) ttCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCa > 1:274670/1‑49 (MQ=255) ttCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc > 1:1157642/1‑69 (MQ=255) ttCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc > 1:1757711/1‑69 (MQ=255) ccAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCa < 1:1385538/47‑1 (MQ=255) ccAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTg < 1:598391/70‑1 (MQ=255) aGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc > 1:782468/1‑71 (MQ=255) gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTTGCGGCAACCTGATcc < 1:3507696/71‑1 (MQ=255) gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCa < 1:1720469/62‑1 (MQ=255) gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc < 1:1430577/71‑1 (MQ=255) gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc < 1:470044/71‑1 (MQ=255) gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc > 1:3010349/1‑70 (MQ=255) gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCa < 1:1574322/57‑1 (MQ=255) gACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc > 1:1316063/1‑63 (MQ=255) aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag > 1:4024266/1‑47 (MQ=255) aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCg < 1:515665/71‑1 (MQ=255) aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCg > 1:3104385/1‑71 (MQ=255) aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCg > 1:3797359/1‑71 (MQ=255) cGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt < 1:2941025/71‑1 (MQ=255) cGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt < 1:2302727/71‑1 (MQ=255) cGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt > 1:3679064/1‑71 (MQ=255) gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt < 1:381791/71‑1 (MQ=255) gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc < 1:2984727/54‑1 (MQ=255) gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGtt > 1:3037191/1‑71 (MQ=255) gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt < 1:3778205/70‑1 (MQ=255) aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGa > 1:699377/1‑56 (MQ=255) aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc > 1:3720045/1‑58 (MQ=255) aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc > 1:1490997/1‑58 (MQ=255) aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc > 1:1938148/1‑71 (MQ=255) aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc > 1:2004059/1‑71 (MQ=255) aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc > 1:3215066/1‑71 (MQ=255) aTCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGttcag < 1:2250979/71‑4 (MQ=255) aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCt > 1:2074084/1‑53 (MQ=255) aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt > 1:3357430/1‑67 (MQ=255) aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg > 1:2775405/1‑71 (MQ=255) aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg > 1:343493/1‑71 (MQ=255) aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg > 1:1399802/1‑71 (MQ=255) cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc > 1:1859216/1‑49 (MQ=255) ttCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCt > 1:1560276/1‑71 (MQ=255) ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAAc < 1:1825225/48‑1 (MQ=255) ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAg > 1:194897/1‑57 (MQ=255) tCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc > 1:127367/1‑44 (MQ=255) tCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt > 1:3326727/1‑70 (MQ=255) tCATCGTAGAACATCCCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTg > 1:1108282/1‑65 (MQ=255) tCATCGTAGAACATCCCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTg > 1:1112987/1‑65 (MQ=255) cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc < 1:1098252/68‑1 (MQ=255) aTCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt > 1:2998414/1‑71 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCt < 1:3612344/64‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc < 1:229421/50‑1 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc > 1:3085872/1‑63 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTg > 1:878957/1‑62 (MQ=255) tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATc < 1:2753595/71‑1 (MQ=255) cGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGAGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCt > 1:2037954/1‑71 (MQ=255) atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt < 1:1614705/70‑1 (MQ=255) gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGAt > 1:507916/1‑65 (MQ=255) cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGt < 1:1095778/71‑1 (MQ=255) | TTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGT > minE/1933014‑1933148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |