| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 244,476 | A→G | 14.0% | Y316H (TAT→CAT) | sbcD ← | exonuclease, dsDNA, ATP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 244,476 | 0 | A | G | 14.0% | 258.6 / 31.5 | 100 | Y316H (TAT→CAT) | sbcD | exonuclease, dsDNA, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (47/39); new base G (6/8); total (53/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
TACCAGCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATCGCGCCACTGTTCCAGC > minE/244408‑244546 | tACCAGCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACt < 1:1075896/71‑1 (MQ=255)tACCAGCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACt < 1:843047/71‑1 (MQ=255) ccAGCAATACTTCGACAGTCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACtca < 1:2859039/71‑1 (MQ=255) ccAGCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACtca > 1:876967/1‑71 (MQ=255) ccAGCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACtca > 1:2710620/1‑71 (MQ=255) ccAGCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACtc < 1:2052306/70‑1 (MQ=255) cAGCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACtcat > 1:3792589/1‑71 (MQ=255) cAGCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACtcat > 1:3374143/1‑71 (MQ=255) gCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATa > 1:2486053/1‑64 (MQ=255) gaaTACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCag < 1:1591959/70‑1 (MQ=255) cAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCag < 1:1149630/71‑1 (MQ=255) aaTACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCag > 1:2826043/1‑70 (MQ=255) tACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACtcat < 1:1549083/65‑1 (MQ=255) aCTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCagta > 1:1960767/1‑69 (MQ=255) cTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATa > 1:1134239/1‑58 (MQ=255) cTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATa > 1:1129435/1‑58 (MQ=255) cTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCagtagt > 1:3333949/1‑70 (MQ=255) cTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACtcat > 1:3234852/1‑63 (MQ=255) cTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCagtagt < 1:3178716/70‑1 (MQ=255) cTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCagtagt > 1:2598560/1‑70 (MQ=255) ttCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTAATCAGTAGTGa > 1:4146100/1‑71 (MQ=255) tCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGAt > 1:1779755/1‑71 (MQ=255) gACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGAtt < 1:3832427/70‑1 (MQ=255) aCAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTc < 1:2728953/71‑1 (MQ=255) aGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCagta > 1:48746/1‑61 (MQ=255) ggCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGAttt > 1:3024507/1‑67 (MQ=255) ggCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTg > 1:320866/1‑63 (MQ=255) aaTGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACtcatca > 1:3727745/1‑54 (MQ=255) aaTGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCagtagt < 1:2995156/59‑1 (MQ=255) aaTGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCagta < 1:2788718/57‑1 (MQ=255) aaTGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATc > 1:3870722/1‑71 (MQ=255) aaTGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATc > 1:888780/1‑71 (MQ=255) tGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGa > 1:2666647/1‑65 (MQ=255) tGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGa > 1:3482946/1‑65 (MQ=255) gATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATccag > 1:1841284/1‑71 (MQ=255) aTTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagc < 1:387882/71‑1 (MQ=255) ttCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCtcatca > 1:166240/1‑49 (MQ=255) ttCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATGCtcatc > 1:3896111/1‑48 (MQ=255) tCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATa > 1:46251/1‑41 (MQ=255) cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATccagcca < 1:1904187/70‑1 (MQ=255) cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATccagcca < 1:2965016/70‑1 (MQ=255) cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATccagcca < 1:904560/70‑1 (MQ=255) cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATccagc > 1:1052797/1‑68 (MQ=255) cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATccagc > 1:3882146/1‑68 (MQ=255) cGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATccagc > 1:2199723/1‑68 (MQ=255) ggTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACtca > 1:2053598/1‑43 (MQ=255) ggTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGa < 1:2175034/71‑1 (MQ=255) gTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATa > 1:1029405/1‑38 (MQ=255) gTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCagtagt < 1:2513375/50‑1 (MQ=255) ttAATGCCTGGATTTTGCGCTGAATATCATGCAGATACtc < 1:1389640/40‑1 (MQ=255) ttAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCagt < 1:3746459/46‑1 (MQ=255) ttAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCagt < 1:1597337/46‑1 (MQ=255) ttAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGAtt < 1:771266/53‑1 (MQ=255) ttAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGAtt < 1:162491/53‑1 (MQ=255) ttAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCTGACa < 1:880702/71‑1 (MQ=255) ttAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACa < 1:1491003/71‑1 (MQ=255) tAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCATCCAGACAg > 1:3530232/1‑71 (MQ=255) tAATGCCTGGATTTTGCGCTGAATATCATGAAGATACTCATCAGTAGTGa > 1:919456/1‑50 (MQ=255) aTGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGa > 1:3428054/1‑48 (MQ=255) aTGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGAt < 1:2133884/49‑1 (MQ=255) tGCCTGGATTTTGCGCTGAGTATCATGCAGATACtcat < 1:1128998/38‑1 (MQ=255) tGCCTGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTc < 1:1761861/51‑1 (MQ=255) tGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGa > 1:927479/1‑65 (MQ=255) tGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGa > 1:2405699/1‑65 (MQ=255) tGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTg > 1:1399627/1‑71 (MQ=255) ccTGGATTTTGCGCTGAATATCATGCAGATACTCATCagta > 1:188849/1‑41 (MQ=255) ccTGGATTTTGCGCTGAATATCATGCAGATACTCATCagta > 1:35335/1‑41 (MQ=255) cTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCt > 1:3560318/1‑71 (MQ=255) cTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCt < 1:2664654/71‑1 (MQ=255) tGGATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGAc < 1:2480836/62‑1 (MQ=255) tGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATcca > 1:3082909/1‑55 (MQ=255) ggATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGa > 1:3462340/1‑42 (MQ=255) gATTTTGCGCTGAATATCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGc > 1:1650136/1‑67 (MQ=255) gATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCt > 1:3771451/1‑71 (MQ=255) aTTTTGCGCTGAATATCATGCAGATACTCATCagta < 1:1418905/36‑1 (MQ=255) aTTTTGCGCTGAATATCATGCAGATACTCATCagta < 1:1549274/36‑1 (MQ=255) aTTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCt < 1:1754765/70‑1 (MQ=255) aTTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTg > 1:952623/1‑71 (MQ=255) ttttGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGc < 1:287876/71‑1 (MQ=255) tGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGa > 1:1270186/1‑70 (MQ=255) gcgcTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGa < 1:3326516/69‑1 (MQ=255) gcgcTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGAt < 1:631749/70‑1 (MQ=255) cgcTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATAc < 1:1066053/71‑1 (MQ=255) cTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACAt < 1:2449510/71‑1 (MQ=255) tGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGa < 1:2040610/65‑1 (MQ=255) tGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACAt > 1:2816433/1‑70 (MQ=255) tGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATc > 1:1993783/1‑71 (MQ=255) gAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATc > 1:1670402/1‑70 (MQ=255) aaTATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagcca < 1:3015022/45‑1 (MQ=255) aaTATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagcca < 1:1537936/45‑1 (MQ=255) aaTATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagcca < 1:1542828/45‑1 (MQ=255) aaTATCATGCAGATGCTCATCAGTAGTGATTTCGATATccagcca < 1:2746914/45‑1 (MQ=255) tatCATGCAGATGCTCATCAGTAGTGATTTCGATATCCAGCCAGAc < 1:210444/46‑1 (MQ=255) cATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAgg > 1:912566/1‑46 (MQ=255) aTGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATCGCGCCACt > 1:639869/1‑71 (MQ=255) tGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGAc > 1:531244/1‑41 (MQ=255) gATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATCGCGCCACTGTTcc < 1:3009518/71‑1 (MQ=255) tACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATCGCGCCACTGTTCCAg > 1:3529391/1‑71 (MQ=255) aCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGa > 1:3065529/1‑49 (MQ=255) aCTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATCGCGCCACTGTTCCAGc > 1:2463646/1‑71 (MQ=255) | TACCAGCAATACTTCGACAGGCAATGATTCGGTTAATGCCTGGATTTTGCGCTGAATATCATGCAGATACTCATCAGTAGTGATTTCGATATCCAGCCAGACAGGTGGCTCCTGCGATACATCGCGCCACTGTTCCAGC > minE/244408‑244546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |