| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,204 | G→A | 12.1% | R413R (CGC→CGT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,204 | 0 | G | A | 12.1% | 185.3 / 14.1 | 66 | R413R (CGC→CGT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (26/32); new base A (5/3); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01 | |||||||||||
CCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAG > minE/1933134‑1933271 | ccTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAg < 1:2905683/71‑1 (MQ=255) tGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACg > 1:3551218/1‑71 (MQ=255) aTCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCgg > 1:778883/1‑70 (MQ=255) cTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt > 1:3302330/1‑71 (MQ=255) tCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt > 1:1475227/1‑71 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCTATGAAGAACTCAAAGCGGTCtg > 1:315032/1‑69 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt > 1:3032118/1‑70 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt > 1:3386400/1‑70 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtg > 1:3916144/1‑69 (MQ=255) aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGa > 1:241460/1‑65 (MQ=255) aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGa > 1:559190/1‑65 (MQ=255) aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCg > 1:3869722/1‑71 (MQ=255) cTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCgg < 1:1341404/52‑1 (MQ=255) cTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCgg < 1:3787812/52‑1 (MQ=255) tCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt < 1:3596602/56‑1 (MQ=255) tCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAc > 1:210115/1‑71 (MQ=255) gCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAc < 1:3019216/69‑1 (MQ=255) cTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGtt < 1:2369899/65‑1 (MQ=255) cTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatc < 1:2614188/71‑1 (MQ=255) aaaGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCg > 1:2196216/1‑45 (MQ=255) aaGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtta > 1:386701/1‑50 (MQ=255) aaGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGa > 1:1012146/1‑52 (MQ=255) aaGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcgtcgt < 1:382135/70‑1 (MQ=255) aaGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcgtcgt < 1:310737/70‑1 (MQ=255) ccGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacg < 1:4146368/71‑1 (MQ=255) cGTTACCGATATCACGACCACCAATGAAGAACTCAAAg < 1:1265064/38‑1 (MQ=255) ttACCGATTTCACGACCACCAATGAAGAACTCAAAg < 1:2501875/36‑1 (MQ=255) ttACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg < 1:2148487/45‑1 (MQ=255) aCCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTa > 1:393359/1‑64 (MQ=255) aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg < 1:2853619/43‑1 (MQ=255) aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgcg > 1:1446853/1‑71 (MQ=255) aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTT‑ACGTCGTTACGACgcgc > 1:2849895/1‑71 (MQ=255) cGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg < 1:3422081/41‑1 (MQ=255) gATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc < 1:1462510/67‑1 (MQ=255) gATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCa < 1:1090304/71‑1 (MQ=255) aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg > 1:835765/1‑71 (MQ=255) ttCACGACCACCAATGAAGACCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg > 1:277583/1‑71 (MQ=255) ttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGtt < 1:663721/48‑1 (MQ=255) tCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:2875756/70‑1 (MQ=255) tCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:1292465/70‑1 (MQ=255) cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga < 1:2515502/71‑1 (MQ=255) gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg > 1:3538469/1‑66 (MQ=255) accaccGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGgggaca > 1:3168460/1‑69 (MQ=255) ccaccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:2176779/71‑1 (MQ=255) caccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACt < 1:3398231/71‑1 (MQ=255) caccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACt < 1:188691/71‑1 (MQ=255) accaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt > 1:1417476/1‑71 (MQ=255) ccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTaa > 1:3805603/1‑40 (MQ=255) caATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTaa > 1:749294/1‑39 (MQ=255) aaTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTg > 1:3490247/1‑71 (MQ=255) aTGAAGAATTCAAAACGGTCGGTGATTTCCGGGtt < 1:3956865/35‑1 (MQ=25) aTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAcgtcgt < 1:1295768/43‑1 (MQ=255) tGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACt < 1:517299/65‑1 (MQ=255) aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTc > 1:821439/1‑65 (MQ=255) aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCg < 1:3500476/70‑1 (MQ=255) agaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGa < 1:900427/71‑1 (MQ=255) agaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGtt < 1:791869/40‑1 (MQ=255) aaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgcg < 1:478069/46‑1 (MQ=255) aaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:2243539/52‑1 (MQ=255) attCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAgggg > 1:1521751/3‑52 (MQ=255) attCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCgg > 1:1986668/3‑52 (MQ=255) cTCAAAGCGGTCTTTGATTTCCGGGTTAACGTCGTTac < 1:366091/38‑1 (MQ=255) cTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac < 1:1159705/38‑1 (MQ=255) cTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGa < 1:3607278/67‑1 (MQ=255) tCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac < 1:3785117/37‑1 (MQ=255) tCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac < 1:768298/37‑1 (MQ=255) aaaaCGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCg > 1:1037387/1‑47 (MQ=19) aaaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGata < 1:2608841/66‑1 (MQ=255) aaaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAg > 1:592188/1‑71 (MQ=255) aaaGCGGTCTGTGATTTCCGGGATAACGTCGTTacga > 1:3417208/1‑37 (MQ=255) | CCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAG > minE/1933134‑1933271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |