| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,237 | A→G | 18.3% | R402R (CGT→CGC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,237 | 0 | A | G | 18.3% | 138.4 / 22.7 | 60 | R402R (CGT→CGC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (21/28); new base G (7/4); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTG > minE/1933171‑1933304 | aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgcg > 1:1446853/1‑71 (MQ=255)aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTT‑ACGTCGTTACGACgcgc > 1:2849895/1‑71 (MQ=255) gATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgc < 1:1462510/67‑1 (MQ=255) gATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCa < 1:1090304/71‑1 (MQ=255) aTTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAg > 1:835765/1‑71 (MQ=255) ttCACGACCACCAATGAAGACCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg > 1:277583/1‑71 (MQ=255) tCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:1292465/70‑1 (MQ=255) tCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:2875756/70‑1 (MQ=255) cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga < 1:2515502/71‑1 (MQ=255) gACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg > 1:3538469/1‑66 (MQ=255) accaccGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGgggaca > 1:3168460/1‑69 (MQ=255) ccaccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:2176779/71‑1 (MQ=255) caccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACt < 1:3398231/71‑1 (MQ=255) caccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACt < 1:188691/71‑1 (MQ=255) accaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt > 1:1417476/1‑71 (MQ=255) aaTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTg > 1:3490247/1‑71 (MQ=255) tGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACt < 1:517299/65‑1 (MQ=255) aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTc > 1:821439/1‑65 (MQ=255) aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCg < 1:3500476/70‑1 (MQ=255) agaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGa < 1:900427/71‑1 (MQ=255) aaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgcg < 1:478069/46‑1 (MQ=255) aaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:2243539/52‑1 (MQ=255) attCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAgggg > 1:1521751/3‑52 (MQ=255) attCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCgg > 1:1986668/3‑52 (MQ=255) cTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGa < 1:3607278/67‑1 (MQ=255) aaaaCGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCg > 1:1037387/1‑47 (MQ=19) aaaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGata < 1:2608841/66‑1 (MQ=255) aaaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAg > 1:592188/1‑71 (MQ=255) aaaGCGGTCTGTGATTTCCGGGATAACGTCGTTacga > 1:3417208/1‑37 (MQ=255) aaacggtcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGt < 1:2930847/68‑1 (MQ=255) ggTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATg < 1:3276287/71‑1 (MQ=255) gTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGa < 1:804056/71‑1 (MQ=255) tgtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga < 1:4118789/42‑1 (MQ=255) tgtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGa < 1:1018364/55‑1 (MQ=255) tgtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAgg > 1:946646/1‑71 (MQ=255) tgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAg < 1:1782671/68‑1 (MQ=255) tgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTc > 1:597976/1‑71 (MQ=255) tgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTc > 1:3032730/1‑71 (MQ=255) tgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGacac < 1:1363723/43‑1 (MQ=16) tgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGt < 1:1036006/70‑1 (MQ=255) gATTTCCGGTTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCg > 1:2912796/1‑71 (MQ=255) aTTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGt > 1:441733/1‑68 (MQ=255) aTTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCgg < 1:694078/71‑1 (MQ=255) aTTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACtt > 1:1314136/1‑43 (MQ=16) tttCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATAtt < 1:2840527/54‑1 (MQ=255) tCCGGGTTAACGTCGTTACGACGCGCCAGCGgaga < 1:2955099/35‑1 (MQ=255) tCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCt > 1:812172/1‑70 (MQ=255) gggTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt < 1:548156/71‑1 (MQ=255) tAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt > 1:2835017/1‑67 (MQ=255) tAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGat > 1:3854578/1‑42 (MQ=16) aaCGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCa > 1:2515140/1‑46 (MQ=255) aaCGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt > 1:1902277/1‑71 (MQ=255) cggcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAtgt < 1:595333/68‑1 (MQ=255) tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGa < 1:1600906/36‑1 (MQ=255) tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGt < 1:1739025/44‑1 (MQ=255) tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGt > 1:2519386/1‑53 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGaa > 1:2960271/1‑48 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCa < 1:114563/62‑1 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt > 1:3825365/1‑71 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt < 1:3072780/71‑1 (MQ=255) attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTcc > 1:3453221/2‑39 (MQ=19) acgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCa > 1:194979/1‑37 (MQ=255) acgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAtgtg < 1:960515/65‑1 (MQ=255) acgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTg > 1:4096231/1‑71 (MQ=255) cgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTc > 1:3843282/1‑68 (MQ=255) gacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAg < 1:626717/44‑1 (MQ=255) gacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAg < 1:2994512/44‑1 (MQ=255) acgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGt < 1:1586248/45‑1 (MQ=255) | ACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTG > minE/1933171‑1933304 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |