| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,276 | G→A | 9.7% | F389F (TTC→TTT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,276 | 0 | G | A | 9.7% | 216.5 / 10.8 | 72 | F389F (TTC→TTT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (35/30); new base A (2/5); total (37/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
GGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCC > minE/1933206‑1933345 | ggTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATg < 1:3276287/71‑1 (MQ=255) gTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGa < 1:804056/71‑1 (MQ=255) tgtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAgg > 1:946646/1‑71 (MQ=255) tgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAg < 1:1782671/68‑1 (MQ=255) tgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTc > 1:3032730/1‑71 (MQ=255) tgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTc > 1:597976/1‑71 (MQ=255) tgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGt < 1:1036006/70‑1 (MQ=255) gATTTCCGGTTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCg > 1:2912796/1‑71 (MQ=255) aTTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGt > 1:441733/1‑68 (MQ=255) aTTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCgg < 1:694078/71‑1 (MQ=255) tCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCt > 1:812172/1‑70 (MQ=255) gggTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt < 1:548156/71‑1 (MQ=255) tAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt > 1:2835017/1‑67 (MQ=255) aaCGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt > 1:1902277/1‑71 (MQ=255) cggcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAtgt < 1:595333/68‑1 (MQ=255) tcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGt > 1:2519386/1‑53 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGaa > 1:2960271/1‑48 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCa < 1:114563/62‑1 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt > 1:3825365/1‑71 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt < 1:3072780/71‑1 (MQ=255) acgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAtgtg < 1:960515/65‑1 (MQ=255) acgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTg > 1:4096231/1‑71 (MQ=255) cgacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTc > 1:3843282/1‑68 (MQ=255) gacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAg < 1:626717/44‑1 (MQ=255) gacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAg < 1:2994512/44‑1 (MQ=255) acgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGt < 1:1586248/45‑1 (MQ=255) cgcgCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc > 1:1345709/1‑71 (MQ=255) cgcgCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc > 1:2553201/1‑71 (MQ=255) cAGCGGAGAAACTTCTGCCGGATATTCAGTAATGa > 1:229937/1‑35 (MQ=255) gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAtg < 1:4143974/52‑1 (MQ=255) gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg < 1:3014788/71‑1 (MQ=255) ggAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGa < 1:1030644/48‑1 (MQ=255) gagaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt > 1:265143/1‑54 (MQ=255) gagaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt > 1:1658654/1‑54 (MQ=255) gagaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCGTCGAAg < 1:536310/71‑1 (MQ=255) gaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg > 1:441372/1‑66 (MQ=255) gaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg > 1:3434060/1‑66 (MQ=255) aaCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtc < 1:407845/70‑1 (MQ=255) aCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt > 1:1310008/1‑43 (MQ=255) aCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt > 1:1487304/1‑43 (MQ=255) aCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtctc > 1:3128126/1‑71 (MQ=255) cTTCTGCCGGATATTCAGTAATGTAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg < 1:780667/62‑1 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCt < 1:3255240/49‑1 (MQ=38) tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTc > 1:817622/1‑47 (MQ=255) tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTg > 1:105183/1‑49 (MQ=255) cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc > 1:159308/1‑52 (MQ=255) cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt > 1:2090698/1‑70 (MQ=255) cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCAAAGATCTCGGt > 1:746323/1‑70 (MQ=255) ccGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAt > 1:3155007/1‑61 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAATAtc < 1:371207/62‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:860749/70‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:858309/70‑1 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCt < 1:680015/40‑1 (MQ=255) cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:1175725/71‑1 (MQ=255) cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:3512688/71‑1 (MQ=255) cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:2631428/71‑1 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:2254113/1‑70 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:2501391/1‑70 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt < 1:358778/71‑1 (MQ=255) atatTCCGTAATAAAGGTAGGCTGAATCAGATGTGCTTCTGCCTCTTCATCAAAGATCTCTGTGACAATAc < 1:795829/71‑1 (MQ=255) ttCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt < 1:3335926/35‑1 (MQ=255) ttCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGc > 1:2923334/1‑71 (MQ=255) ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc > 1:144352/3‑70 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCa > 1:3785069/1‑39 (MQ=255) cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCa < 1:1707106/70‑1 (MQ=255) aGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:2093972/62‑1 (MQ=255) aGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:2544326/62‑1 (MQ=255) aGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa < 1:3744619/71‑1 (MQ=255) tAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGa > 1:2526580/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg > 1:1578023/1‑51 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt < 1:2412059/53‑1 (MQ=255) aTGAAGGTAGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGc > 1:1472087/1‑64 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATAc < 1:2278648/60‑1 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGaa > 1:2384905/1‑42 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGaa > 1:4097567/1‑42 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg > 1:764337/1‑49 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:849269/1‑58 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:2230538/1‑58 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:3981425/1‑70 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:3735313/1‑70 (MQ=255) | GGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCC > minE/1933206‑1933345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |