| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 271,886 | G→A | 8.9% | G75G (GGC→GGT) | yajL ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 271,886 | 0 | G | A | 8.9% | 204.1 / 8.3 | 67 | G75G (GGC→GGT) | yajL | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (29/32); new base A (3/3); total (32/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
GAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGC > minE/271818‑271952 | gAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGc < 1:600335/70‑1 (MQ=255) aCGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGccac < 1:3902898/71‑1 (MQ=255) gTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAg > 1:3037755/1‑71 (MQ=255) ttgAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAgg < 1:390485/69‑1 (MQ=255) tGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAgg > 1:131163/1‑71 (MQ=255) gAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGgc < 1:3086702/70‑1 (MQ=255) gAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGgc < 1:620393/70‑1 (MQ=255) aCTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGgcatc < 1:2266098/71‑3 (MQ=255) gTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCAc > 1:1134553/1‑70 (MQ=255) tttAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAgg < 1:2597087/63‑1 (MQ=255) tttAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACg < 1:2250045/70‑1 (MQ=255) ttAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGAt > 1:3708739/1‑71 (MQ=255) tAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGgc > 1:1930969/1‑62 (MQ=255) tAACGGTTTCAACCAGCAGAGTGCTATAGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGAt > 1:1725988/1‑70 (MQ=255) aCGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCa < 1:817290/70‑1 (MQ=255) tttCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCa < 1:883385/71‑1 (MQ=255) tttCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCa < 1:878518/71‑1 (MQ=255) ttCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCa > 1:707070/1‑70 (MQ=255) cAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCata < 1:503714/70‑1 (MQ=255) cagcagAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGc < 1:1539474/71‑1 (MQ=255) cagcagAGTGCTATCGCGAAAACACTCCGCGCCTTTAATACCACCAGGCAGCACGATCACGTCATATTCgt < 1:3397755/71‑2 (MQ=255) cagcagAGTGCTATCGCGAAAACACTCCGCGCCTTTAATACCACCAGGCAGCACGATCACGTCATATTCGc < 1:3379451/71‑1 (MQ=255) agTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACg < 1:1769473/49‑1 (MQ=255) gTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGc < 1:2586372/71‑1 (MQ=255) tGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCAc < 1:1560935/46‑1 (MQ=255) tATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACg < 1:758778/44‑1 (MQ=255) tATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGc < 1:3207489/67‑1 (MQ=255) aTCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACtt > 1:2910790/1‑71 (MQ=255) tCGCGAAAACACTCCTCGCCTTTAATACCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACt < 1:1110393/69‑1 (MQ=255) tCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACg < 1:4055763/42‑1 (MQ=255) tCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCatat > 1:2825761/1‑54 (MQ=255) tCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCat < 1:2800330/52‑1 (MQ=255) tCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGc < 1:1590400/65‑1 (MQ=255) tCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACt < 1:2344924/69‑1 (MQ=255) tCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTc > 1:2654362/1‑71 (MQ=255) tCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTc > 1:2250838/1‑71 (MQ=255) tCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTc > 1:3949781/1‑71 (MQ=255) cgcgAAAACACTCCGCGCCTTTAATGCCACCAGgca > 1:1389917/1‑36 (MQ=255) cgcgAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGcc < 1:681823/65‑1 (MQ=255) tcgAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCAc > 1:4077837/2‑39 (MQ=255) gcgAAGACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCg > 1:1642114/1‑55 (MQ=255) gcgAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCa > 1:3276434/1‑65 (MQ=255) cgAAATCACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGAc > 1:1931160/1‑71 (MQ=255) cgAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGAc > 1:1653794/1‑71 (MQ=255) cgAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGAc > 1:1122304/1‑71 (MQ=255) gAAAACACTCCGCGCCTTTAATGCCATCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGAc > 1:598172/1‑70 (MQ=255) gAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGAt > 1:2226377/1‑40 (MQ=255) gAAAACACTCCGCGCCTTTAATACCACCAGGCAGCACGATCACGTCATATTCGCCATc > 1:1848033/1‑58 (MQ=255) gAAAACACTCCGCGCCTTTAATACCACCAGGCAGCACGATCACGTCATATTCGCCATc > 1:3771193/1‑58 (MQ=255) aaCACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCat > 1:3914271/1‑45 (MQ=255) aaCACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCat > 1:416473/1‑45 (MQ=255) acacTCCGCGCCTTTAATGCCACCAGGCAGCACGATCa < 1:2534328/38‑1 (MQ=255) acacTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAgcg > 1:176022/1‑71 (MQ=255) acacTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAgcg > 1:2582990/1‑71 (MQ=255) acTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTc < 1:1545540/46‑1 (MQ=255) acTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTc < 1:644333/46‑1 (MQ=255) cTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGc < 1:1929902/47‑1 (MQ=255) tCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGc < 1:1509109/53‑1 (MQ=255) tCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCg < 1:4131885/60‑1 (MQ=255) ccGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATAtt > 1:3768068/1‑42 (MQ=255) ccGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATAtt > 1:146848/1‑42 (MQ=255) cgcgCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCAc > 1:4047318/1‑54 (MQ=255) ccTTTAATACCACCAGGCAGCACGATCACGTCATATTCGCCAt > 1:3996843/1‑43 (MQ=255) cTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGc > 1:2374528/1‑70 (MQ=255) tAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATAAGCCACTTCGACCAGCGGCGCATCCGCcag < 1:4089664/70‑1 (MQ=255) aaTGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCcagc < 1:1731384/70‑1 (MQ=255) aaTGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCcagc < 1:1053606/70‑1 (MQ=255) | GAACGGTGGAACTGTTTAACGGTTTCAACCAGCAGAGTGCTATCGCGAAAACACTCCGCGCCTTTAATGCCACCAGGCAGCACGATCACGTCATATTCGCCATCAGCCACTTCGACCAGCGGCGCATCCGCCAGC > minE/271818‑271952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |