| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,324 | G→T | 5.8% | T373T (ACC→ACA) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,324 | 0 | G | T | 5.8% | 349.5 / 3.9 | 103 | T373T (ACC→ACA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (54/43); new base T (2/4); total (56/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
CTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCG > minE/1933257‑1933391 | cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt > 1:2090698/1‑70 (MQ=255)cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCAAAGATCTCGGt > 1:746323/1‑70 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:858309/70‑1 (MQ=255) cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:1175725/71‑1 (MQ=255) cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:2631428/71‑1 (MQ=255) cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:3512688/71‑1 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:2254113/1‑70 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:2501391/1‑70 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt < 1:358778/71‑1 (MQ=255) atatTCCGTAATAAAGGTAGGCTGAATCAGATGTGCTTCTGCCTCTTCATCAAAGATCTCTGTGACAATAc < 1:795829/71‑1 (MQ=255) ttCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGc > 1:2923334/1‑71 (MQ=255) cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCa < 1:1707106/70‑1 (MQ=255) aGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:2544326/62‑1 (MQ=255) aGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa < 1:3744619/71‑1 (MQ=255) tAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGa > 1:2526580/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg > 1:1578023/1‑51 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt < 1:2412059/53‑1 (MQ=255) aTGAAGGTAGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGc > 1:1472087/1‑64 (MQ=255) taaaGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAc < 1:4032444/53‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATAc < 1:2278648/60‑1 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg > 1:764337/1‑49 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:849269/1‑58 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:2230538/1‑58 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:3735313/1‑70 (MQ=255) gAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:3981425/1‑70 (MQ=255) aGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGt < 1:223396/49‑1 (MQ=37) gTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATAc > 1:2142712/1‑55 (MQ=255) gTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc > 1:3866125/1‑59 (MQ=255) gTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGc > 1:3615909/1‑70 (MQ=255) gCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCtcttc < 1:2266095/71‑1 (MQ=255) gCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCtctt > 1:408421/1‑70 (MQ=255) tGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCtcttct > 1:146083/1‑70 (MQ=255) gAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAg < 1:1381408/62‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCaa < 1:2300994/71‑1 (MQ=255) aTCAGATGTGCTTCTGCCACTTCTTCGAAGCTCTCGGTAACGATACGGCCCAGACCCCAGct > 1:809513/1‑62 (MQ=255) aTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATAc > 1:2175364/1‑46 (MQ=255) aTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAAc > 1:845314/1‑71 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:2878206/42‑1 (MQ=255) ccAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:2488100/41‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc > 1:1353315/1‑38 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc < 1:1832525/53‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGt < 1:2469012/70‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg < 1:3343223/71‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg < 1:1541852/71‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg < 1:3942020/71‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg < 1:3982203/71‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg < 1:4064049/71‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg > 1:1269669/1‑71 (MQ=255) gATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:3235575/1‑39 (MQ=255) gATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTgg < 1:4045724/71‑1 (MQ=255) aTGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:1139404/38‑1 (MQ=255) aTGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc > 1:3665814/1‑53 (MQ=255) aTGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGctct < 1:3610464/59‑1 (MQ=255) aTGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc > 1:1155314/1‑63 (MQ=255) tgtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:1852934/1‑37 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGa > 1:337641/1‑69 (MQ=255) tgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg < 1:4144918/70‑1 (MQ=255) gCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCa > 1:3144897/1‑50 (MQ=255) gCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:1222754/1‑71 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc < 1:2185833/40‑1 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCaa > 1:2050678/1‑60 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc < 1:1991402/70‑1 (MQ=255) cTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCa > 1:747605/1‑42 (MQ=16) ttCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa > 1:1831073/1‑71 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCaa > 1:1304271/1‑57 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCaa > 1:1214733/1‑57 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGc > 1:2456128/1‑66 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa < 1:72499/69‑1 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa < 1:11766/69‑1 (MQ=255) tGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa > 1:1364799/1‑68 (MQ=255) gCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:2856586/1‑42 (MQ=255) ccACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAg < 1:2902741/69‑1 (MQ=255) cACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATa < 1:826830/67‑1 (MQ=255) aCTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGa > 1:84642/1‑58 (MQ=255) aCTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt > 1:374953/1‑70 (MQ=255) aCTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt > 1:2993763/1‑70 (MQ=255) tcttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg > 1:2160279/1‑70 (MQ=255) cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc < 1:3599303/70‑1 (MQ=255) cttcGAAGATCTCGGTAACGATACGGCCCAGACACCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc > 1:2667430/1‑70 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg < 1:4034701/51‑1 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg < 1:7968/51‑1 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGc > 1:1548436/1‑56 (MQ=255) ttcGAAGATCTCGGTAACGATACGCCCCAGACCCCAGCTCTTCTCAACGt < 1:1103487/50‑1 (MQ=255) ttcGAACATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCaa > 1:4338/1‑71 (MQ=255) cGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCaa > 1:44563/1‑45 (MQ=255) cGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCa < 1:2186850/68‑1 (MQ=255) cGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCa < 1:4113266/68‑1 (MQ=255) gAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTg < 1:1437023/71‑1 (MQ=255) aGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCaa > 1:1160441/1‑42 (MQ=255) gATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg < 1:12779/45‑1 (MQ=255) gATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCtt > 1:3687825/1‑71 (MQ=255) gATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCtt > 1:3092007/1‑71 (MQ=255) aTCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg < 1:200227/44‑1 (MQ=255) tctcGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTg > 1:704231/1‑66 (MQ=255) tctcGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTg > 1:71917/1‑66 (MQ=255) ctcGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTgg > 1:1969509/1‑43 (MQ=255) ctcGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGa > 1:3132611/1‑44 (MQ=255) tcGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc < 1:3741170/59‑1 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc < 1:3822186/67‑1 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTATCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg > 1:3090198/1‑68 (MQ=255) | CTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCG > minE/1933257‑1933391 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |