| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,581 | T→G | 6.1% | K288Q (AAA→CAA) ‡ | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,581 | 0 | T | G | 6.1% | 267.2 / 12.2 | 99 | K288Q (AAA→CAA) ‡ | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (54/39); new base G (4/2); total (58/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
TCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAC > minE/1933514‑1933646 | tCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTg > 1:959493/1‑69 (MQ=255) ttACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGc > 1:2941054/1‑71 (MQ=255) ttACCGAGAATATCCTGTGCCAGAGTACGGAACAGAGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGc > 1:1184279/1‑71 (MQ=255) aCCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGc < 1:436360/69‑1 (MQ=255) aCCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCg > 1:1048979/1‑70 (MQ=255) aCCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCg > 1:3987714/1‑70 (MQ=255) ccGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTa > 1:3244948/1‑62 (MQ=255) ccGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGt > 1:3504792/1‑70 (MQ=255) cGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTa < 1:3081496/61‑1 (MQ=255) gagaATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAg > 1:3251239/1‑71 (MQ=255) gagaATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAg > 1:787454/1‑71 (MQ=255) aaTATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa > 1:3586161/1‑71 (MQ=255) tatCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:493825/1‑71 (MQ=255) atCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGc > 1:803082/1‑66 (MQ=255) atCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:1540856/1‑70 (MQ=255) atCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:710732/1‑70 (MQ=255) tgCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTa < 1:2126579/48‑1 (MQ=255) tgCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGtt > 1:3752500/1‑71 (MQ=255) tgCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGtt > 1:2024923/1‑71 (MQ=255) tgCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGtt > 1:3873091/1‑71 (MQ=255) gCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGc > 1:311818/1‑59 (MQ=255) gCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa < 1:743968/65‑1 (MQ=255) gCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTc > 1:808657/1‑71 (MQ=255) ccAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTc > 1:3769239/1‑70 (MQ=255) cAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTaga > 1:2596937/1‑65 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:2654301/71‑1 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:405187/71‑1 (MQ=255) gagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTc > 1:2552437/1‑67 (MQ=255) gtgcGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCcatcat < 1:4062946/68‑1 (MQ=255) gTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGtt < 1:4030477/64‑1 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTa < 1:3224893/40‑1 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGc < 1:2990111/52‑1 (MQ=255) gcGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCcatcattg > 1:805300/2‑69 (MQ=255) aCGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATgg > 1:777398/1‑71 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATc > 1:1096470/1‑41 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCt > 1:533369/1‑42 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGcc > 1:2928884/1‑51 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:582045/1‑71 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt < 1:3369666/71‑1 (MQ=255) gAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatc > 1:4052373/1‑64 (MQ=255) aCAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCcatc > 1:1070820/1‑62 (MQ=255) aCAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAc > 1:1482309/1‑71 (MQ=255) cAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatc < 1:272688/61‑1 (MQ=255) cAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAct > 1:3825783/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTagag > 1:2408506/1‑52 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:1261435/71‑1 (MQ=255) gATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTgg > 1:3818034/1‑71 (MQ=255) aTTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa < 1:1948008/46‑1 (MQ=255) ttCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGt > 1:2920777/1‑71 (MQ=255) tCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTg > 1:3181064/1‑60 (MQ=255) tCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGt > 1:831731/1‑70 (MQ=255) cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGtt > 1:2669447/1‑48 (MQ=255) cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTgg > 1:909813/1‑67 (MQ=255) gTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATg < 1:2209943/71‑1 (MQ=255) gTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATg < 1:852328/71‑1 (MQ=255) aGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTgg < 1:2262911/62‑1 (MQ=255) gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg < 1:3572921/70‑1 (MQ=255) gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGt < 1:89657/71‑1 (MQ=255) gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGt < 1:1667434/71‑1 (MQ=255) cTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTagag > 1:400692/1‑39 (MQ=255) cTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGtt > 1:406979/1‑41 (MQ=255) cTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTAt < 1:1580681/65‑1 (MQ=255) cTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTa > 1:18510/1‑71 (MQ=255) tCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTg < 1:1292786/51‑1 (MQ=255) tCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTAc > 1:3990410/1‑71 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGG‑TATGACGTACGGa < 1:1870443/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACTTACGGa < 1:533436/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:968724/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:1979392/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:3216173/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:3899779/71‑1 (MQ=255) aTCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAAcag > 1:3822874/1‑68 (MQ=255) aTCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa < 1:2550477/71‑1 (MQ=255) tCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTc > 1:1651916/1‑37 (MQ=12) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:590360/1‑70 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:3124829/68‑1 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAAt < 1:2070404/71‑1 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAAt < 1:310532/71‑1 (MQ=255) aGATCTTTGTAAACTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:2915336/1‑44 (MQ=255) gATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:2337203/67‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatcat < 1:2705904/39‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:1104750/36‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:2762994/36‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTg < 1:2020246/43‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:2238646/66‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:3417673/1‑71 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:1151704/1‑71 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:870379/1‑71 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:3634999/1‑71 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:3863377/1‑71 (MQ=255) aTCTTTGTAATATGCGTAAGCCATGTAGAGTTCCATAATGGTGAACTCTGGGTTATg < 1:1832478/57‑1 (MQ=255) aTCATTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:1360294/36‑1 (MQ=255) cTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGAc > 1:617691/1‑57 (MQ=255) cTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:356757/1‑66 (MQ=255) ttGTAATCTGCGTAAGCCATGTAGAGTTCcatcat > 1:2554648/1‑35 (MQ=255) ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg > 1:3621195/1‑36 (MQ=255) ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg < 1:1406816/36‑1 (MQ=255) ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg > 1:2032029/1‑36 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:2881580/44‑1 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc < 1:1380633/66‑1 (MQ=255) | TCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAC > minE/1933514‑1933646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |